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Cell No. : Cell Name
RCB0407 : WS2TKB  update : 2023/01/04
CommentWerner's syndrome
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Bannai, Shiro
Originator Bannai, S. & Yamashita, K.
Year of deposit 1989
Another name WS2
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Female
Age at sampling 25 years
Tissue skin
Metastatic ability No
Genetics Werner's syndrome
Classification normal
Lifespan finite
Crisis PDL 12-15 PDL
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_J712
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM ALPHA + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Achoreplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 43-47(50) : 43(1),44(4),45(9),46(34),47(2)
STR(human) OK
Reference information Reference 0
User's Publication 0


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