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Cell No. : Cell Name
RCB0405 : NIAS-LeSe-11  update : 2024/01/17
CommentSusceptible to insect viruses.
Comment from the depositor
Terms and conditionsNo restriction, but the user needs depositor's approval to use for any commercial purpose.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Mitsuhashi, Jun
Originator Mitsuhashi, Jun
Year of deposit 1989
Animal _armyworm < Insect
Strain name common armyworm
Genus Pseudaletia
Species separata(Walker)
Tissue fat body
Classification other
Year of origin 1978
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_Z368
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium MM (or Schneider's) + 3% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Isozyme LD, NP
Reference information Reference 3
User's Publication 0


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Reference
1092  MITSUHASHI J.  Simplification of media and utilization of sugars by insect cells in cultures.  Invertebrate and Fish Tissue Culture  1988  15-18   
1182  Mituhashi, Jun  Isolation of a Continuous Cell Line from Larval Fat Bodies of an Arctiid moth, Spilarctia seriatopunctata(Insecta,Lepidoptera,Arctiidae).  Zoological Science  1984  1:415-419   
1084  Mitsuhashi, Jun  A Continuous Cell Line Derived from Fat Bodies of the Common Armyworm, Leucania Separata (Lepidoptera:Noctuidae).  Appl Ent Zool  1983  18:533-539    DOI: 10.1303/aez.18.533

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