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Cell No. : Cell Name
RCB0403 : CHO-K1 (SC)  update : 2022/03/14
CommentA subline of CHO-K1 (RCB0285). Suspension culture is possible.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao
Originator Ohno, T. & Kurita, K.
Year of deposit 1989
Animal _hamster < Mammals
Strain name Chinese
Tissue ovary
Classification transformed
History Maintained in suspension cultu
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_U864
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium MEM (suspension, 2mM L-Glutamine) + 5% HamF12 + 5% FBS
Antibiotics Free
Passage method dilution
Designated culture dish FALCON 1029→ spinner bottle
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
deposit info
lot info
Reference information Reference 0
User's Publication 6

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User's Publication
14072  Fujii G, Kurashina Y, Terao Y, Azuma T, Morikawa A, Kodeki K, Takahara O, Takemura K.  Suspension culture in a T-flask with acoustic flow induced by ultrasonic irradiation  Ultrason Sonochem  2021  73:105488  PubMed ID: 33607592   DOI: 10.1016/j.ultsonch.2021.105488
20305  Oyama T, Imashiro C, Kuriyama T, Usui H, Ando K, Azuma T, Morikawa A, Kodeki K, Takahara O, Takemura K.  Acoustic streaming induced by MHz-frequency ultrasound extends the volume limit of cell suspension culture.  J Acoust Soc Am  2021  149(6):4180  PubMed ID: 34241472   DOI: 10.1121/10.0005197
20342  Wójtowicz K, Czogalla A, Trombik T, Łukaszewicz M.  Surfactin cyclic lipopeptides change the plasma membrane composition and lateral organization in mammalian cells.  Biochim Biophys Acta Biomembr  2021  1863(12):183730  PubMed ID: 34419486   DOI: 10.1016/j.bbamem.2021.183730
11708  Yuta Kurashina, Chikahiro Imashiro, Makoto Hirano, Taiki Kuribara, Kiichiro Totani, Kiyoshi Ohnuma, James Friend, Kenjiro Takemura   Enzyme-free release of adhered cells from standard culture dishes using intermittent ultrasonic traveling waves  Communications Biology  2019  2(1):393  PubMed ID: 31701022   DOI: 10.1038/s42003-019-0638-5
6960  Hiromi Minbu, Haruki Mizuno, Yuki Shibuya, Akihito Ochiai, Masayuki Taniguchi, Takaaki Tanaka  Poly (l-lactic acid) depth filter membrane prepared by nonsolvent-induced phase separation with the aid of a nonionic surfactant  JOURNAL OF CHEMICAL ENGINEERING OF JAPAN  2019  52:75-82    DOI: 10.1252/jcej.18we084
17910  Yuka Tawara, Masa-aki Kitajima, Masatoshi Togami  Serum-Free Media for Large-Scale Suspension Cultures  Animal Cell Technology: Basic & Applied Aspects  1993  397-401    DOI: 10.1007/978-94-011-2044-9_54

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