Cell No. : Cell Name
RCB0403 : CHO-K1 (SC)
update : 2024/09/19
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| Comment | A subline of CHO-K1 (RCB0285). Suspension culture is possible. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ohno, Tadao
|
| Originator |
Ohno, T. & Kurita, K.
|
| Year of deposit |
1989
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Chinese
|
| Tissue |
ovary
|
| Classification |
transformed
|
| History |
Maintained in suspension cultu
|
|
Lifespan |
infinite
|
| Morphology |
other
|
| Cellosaurus(Expasy) |
CVCL_U864
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent/Suspension cells
|
| Culture medium |
|
MEM (suspension, 2mM L-Glutamine) + 5% HamF12 + 5% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dilution
|
| Designated culture dish |
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FALCON 1029→ spinner bottle
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Culture information
|
Passage ratio |
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1 : 8 split
|
| SC frequency |
|
Subculture : 1-2 times/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
0
|
| User's Publication |
6
|
| User's Publication |
|
14072
Fujii G, Kurashina Y, Terao Y, Azuma T, Morikawa A, Kodeki K, Takahara O, Takemura K.
Suspension culture in a T-flask with acoustic flow induced by ultrasonic irradiation
Ultrason Sonochem
2021
73:105488
PubMed ID: 33607592
DOI: 10.1016/j.ultsonch.2021.105488
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20305
Oyama T, Imashiro C, Kuriyama T, Usui H, Ando K, Azuma T, Morikawa A, Kodeki K, Takahara O, Takemura K.
Acoustic streaming induced by MHz-frequency ultrasound extends the volume limit of cell suspension culture.
J Acoust Soc Am
2021
149(6):4180
PubMed ID: 34241472
DOI: 10.1121/10.0005197
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20342
Wójtowicz K, Czogalla A, Trombik T, Łukaszewicz M.
Surfactin cyclic lipopeptides change the plasma membrane composition and lateral organization in mammalian cells.
Biochim Biophys Acta Biomembr
2021
1863(12):183730
PubMed ID: 34419486
DOI: 10.1016/j.bbamem.2021.183730
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11708
Yuta Kurashina, Chikahiro Imashiro, Makoto Hirano, Taiki Kuribara, Kiichiro Totani, Kiyoshi Ohnuma, James Friend, Kenjiro Takemura
Enzyme-free release of adhered cells from standard culture dishes using intermittent ultrasonic traveling waves
Communications Biology
2019
2(1):393
PubMed ID: 31701022
DOI: 10.1038/s42003-019-0638-5
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6960
Hiromi Minbu, Haruki Mizuno, Yuki Shibuya, Akihito Ochiai, Masayuki Taniguchi, Takaaki Tanaka
Poly (l-lactic acid) depth filter membrane prepared by nonsolvent-induced phase separation with the aid of a nonionic surfactant
JOURNAL OF CHEMICAL ENGINEERING OF JAPAN
2019
52:75-82
DOI: 10.1252/jcej.18we084
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17910
Yuka Tawara, Masa-aki Kitajima, Masatoshi Togami
Serum-Free Media for Large-Scale Suspension Cultures
Animal Cell Technology: Basic & Applied Aspects
1993
397-401
DOI: 10.1007/978-94-011-2044-9_54
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