Cell No. : Cell Name
RCB0402 : HeLa·P3
update : 2022/03/14
|
| Comment | Protein- free, serm- free, lipid-free medium growing |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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|
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Basic information
|
Depositor |
Takaoka, Toshiko
|
| Originator |
Katsuta, Hajimu
|
| Year of deposit |
1989
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
cervix
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
| Cellosaurus(Expasy) |
CVCL_2937
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DM-160AU
|
| Antibiotics |
|
Free
|
|
Passage method |
|
dispase (1000U/ml)
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : once/3-4 weeks, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
DM-160AU : 2 x FM1 = 1 : 1
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
55-63(31) : 55(3),56(5),57(4),58(6),59(5),60(5)/
|
| STR(human) |
|
OK
|
| Reference information |
Reference |
6
|
| User's Publication |
3
|
| Reference |
|
1002
Katsuta,H. and Takaoka,S.
Protein-and Lipid-Free Synthetic Media for The Cultivation of Mammalian Cells in Tissue Culture.
Nutritional Requirements of Cultured Cells.
1978
257-275
|
|
1009
Takaoka T, Katsuta H
Long-term cultivation of mammalian cell strains in protein- and lipid-free chemically defined synthetic media.
Exp Cell Res
1971
67:295-304
PubMed ID: 4328993
DOI: 10.1016/0014-4827(71)90412-5
|
|
1011
Kagawa Y, Takaoka T, Katsuta H
Absence of Essential Fatty Acids in Mammalian Cell Strains Cultured in Lipid- and Protein-Free Chemically Definde Synthetic Media.
J Biochem (Tokyo)
1970
68:133-136
PubMed ID: 5452761
|
|
998
Katsuta,H., Takaoka,T., Furukawa,T., and Kawane,M.
Cultivation of Strain HeLa Cells (Cervical of Human Uterus) in A Protein-Free Medium.
Jpn J Exp Med
1960
30:147-157
PubMed ID: 14404775
|
|
997
Katsuta,H., Takaoka,T., Kuwabara,H., and Kuwabara,S.
Cultivation of Strain HeLa Cells (Human Cervical Carcinoma) in The Simplified Replicate Tissue Culture.
Jpn J Exp Med
1959
29:493-505
PubMed ID: 14404771
|
|
150
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res
1952
12:264-265
|
| User's Publication |
|
6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
DOI: 10.1111/j.1749-0774.2009.00071.x
|
|
2531
Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG.
Short tandem repeat profiling provides an international reference standard for human cell lines.
Proc Natl Acad Sci U S A.
2001
98(14):8012-7
PubMed ID: 11416159
DOI: 10.1073/pnas.121616198
|
|
1567
Ohno, T., Kurita, K., Abe, S., Eimori, N., and Ikawa, Y.
A simple Freezing Medium for Serum-Free Cultured Cells
Cytotechnology
1988
1:257-260
PubMed ID: 22359122
DOI: 10.1007/BF00145029
|
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