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Cell No. : Cell Name
RCB0357 : HR-3Y1-2  update : 2022/03/14
CommentA subline of 3Y1 cell line. Transformed by v-Ha-ras oncogene.
Comment from the depositor
Terms and conditionsNo restriction, but required to cite ref. (Cell Struct Funct 1990;15:211-219) and to send a reprint to originator.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kimura, Genki
Originator Kimura, Genki
Year of deposit 1989
Animal rat < Mammals
Strain name Fischer
Gender Female
Age at sampling embryo
Tissue embryo/fetus, whole
Classification transformed
Recombinant recombinant
Exogene v-Ha-ras, pBR322
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_8097
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 1


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Reference
1057  Shimura H, Mitsudomi T, Matsuzaki A, Kabemura M, Okuda A, Kimura G  Transformation by v-H-ras does not restore proliferation of a set of temperature-sensitive cell-cycle mutants of rat 3Y1 fibroblasts.  Cell Struct Funct  1990  15:211-9  PubMed ID: 2257639  

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User's Publication
2911  Itano, Naoki, Sawai, Takahiro, Atsumi, Fukiko, Miyaishi, Osamu, Taniguchi, Shun'ichiro, Kannagi, Reiji, Hamaguchi, Michinari, Kimata, Koji  Selective expression and functional characteristics of three mammalian hyaluronan synthases in oncogenic malignant transformation.  J Biol Chem  2004  279:18679-87  PubMed ID: 14724275   DOI: 10.1074/jbc.M313178200



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