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Cell No. : Cell Name
RCB0357 : HR-3Y1-2 update : 2022/03/14 |
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| Comment | A subline of 3Y1 cell line. Transformed by v-Ha-ras oncogene. | |||
| Comment from the depositor | ||||
| Terms and conditions | No restriction, but required to cite ref. (Cell Struct Funct 1990;15:211-219) and to send a reprint to originator. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | Kimura, Genki | ||
| Originator | Kimura, Genki | |||
| Year of deposit | 1989 | |||
| Animal | _rat < Mammals | |||
| Strain name | Fischer | |||
| Gender | Female | |||
| Age at sampling | embryo | |||
| Tissue | embryo/fetus, whole | |||
| Classification | transformed | |||
| Recombinant | recombinant | |||
| Exogene | v-Ha-ras, pBR322 | Lifespan | infinite | |
| Morphology | fibroblast-like | |||
| Cellosaurus(Expasy) | CVCL_8097 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | DMEM (low glucose) + 10% FBS | |||
| Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 8 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma/Acholeplasma | (-) | |||
| Animal PCR | OK | |||
| Isozyme | LD, NP | |||
| Reference information | Reference | 1 | ||
| User's Publication | 1 | |||
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