Cell No. : Cell Name RCB0353 : SR-3Y1-1  update : 2022/03/14
Comment			A subline of 3Y1 cell line. Transformed by Rous sarcoma virus.
Comment from the depositor
Terms and conditions			No restriction, but required to cite ref. (Biomedical Research 1988;9:181-197) and to send a reprint to originator.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Kimura, Genki
		Originator	Kimura, Genki
		Year of deposit	1989
		Animal	_rat < Mammals
		Strain name	Fischer
		Gender	Female
		Age at sampling	embryo
		Tissue	embryo/fetus, whole
		Classification	transformed
		Recombinant	recombinant
		Exogene	Rous sarcoma virus
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_8115
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (low glucose) + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP
Reference information		Reference	2
		User's Publication	2

Top

Reference

1062  Zaitsu, H., Tanaka, H., Mitsudomi, T., Matsuzaki, A., Ohtsu, M. and Kimura, G.  Differences in proliferation PropertiesAmong Sublines of Rat 3Y1 Fibroblasts Transformed by Various Agents in Vitro  Biomedical Research  1988  9:181-197    DOI: 10.2220/biomedres.9.181

1059  Tanaka H1, Zaitsu H, Onodera K, Kimura G.  Influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3Y1 fibroblasts and their derivatives transformed by a wide variety of agents.  J Cell Physical  1988  136(3):421-30  PubMed ID: 2844831   DOI: 10.1002/jcp.1041360305

Top

User's Publication

17963  Sasaki H, Nagura K, Ishino M, Tobioka H, Kotani K, Sasaki T.  Cloning and characterization of cell adhesion kinase beta, a novel protein-tyrosine kinase of the focal adhesion kinase subfamily  J Biol Chem  1995  270(36):21206-19  PubMed ID: 7673154   DOI: 10.1074/jbc.270.36.21206

17215  M Ishino, H Sasaki, T Sasaki  Multiple Proline-rich Regions of GAP-associated Phosphoprotein p62 Bind with Different Affinities to the Src Homology 3 Domains of Fyn and Src  Tumor Research  1994  29:59-75