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Cell No. : Cell Name
RCB0353 : SR-3Y1-1  update : 2022/03/14
CommentA subline of 3Y1 cell line. Transformed by Rous sarcoma virus.
Comment from the depositor
Terms and conditionsNo restriction, but required to cite ref. (Biomedical Research 1988;9:181-197) and to send a reprint to originator.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kimura, Genki
Originator Kimura, Genki
Year of deposit 1989
Animal _rat < Mammals
Strain name Fischer
Gender Female
Age at sampling embryo
Tissue embryo/fetus, whole
Classification transformed
Recombinant recombinant
Exogene Rous sarcoma virus
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_8115
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 2
User's Publication 2

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1062  Zaitsu, H., Tanaka, H., Mitsudomi, T., Matsuzaki, A., Ohtsu, M. and Kimura, G.  Differences in proliferation PropertiesAmong Sublines of Rat 3Y1 Fibroblasts Transformed by Various Agents in Vitro  Biomedical Research  1988  9:181-197    DOI: 10.2220/biomedres.9.181
1059  Tanaka H1, Zaitsu H, Onodera K, Kimura G.  Influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3Y1 fibroblasts and their derivatives transformed by a wide variety of agents.  J Cell Physical  1988  136(3):421-30  PubMed ID: 2844831   DOI: 10.1002/jcp.1041360305

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User's Publication
17963  Sasaki H, Nagura K, Ishino M, Tobioka H, Kotani K, Sasaki T.  Cloning and characterization of cell adhesion kinase beta, a novel protein-tyrosine kinase of the focal adhesion kinase subfamily  J Biol Chem  1995  270(36):21206-19  PubMed ID: 7673154   DOI: 10.1074/jbc.270.36.21206
17215  M Ishino, H Sasaki, T Sasaki  Multiple Proline-rich Regions of GAP-associated Phosphoprotein p62 Bind with Different Affinities to the Src Homology 3 Domains of Fyn and Src  Tumor Research  1994  29:59-75   

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