Back Back
Cell No. : Cell Name
RCB0343 : SV-3Y1-C66  update : 2022/03/11
CommentA subline of 3Y1 cell line. Transformed by SV40 virion.
Comment from the depositor
Terms and conditionsNo restriction, but required to cite ref. (Biomedical Research 1988;9:181-197) and to send a reprint to originator.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kimura, Genki
Originator Kimura, Genki
Year of deposit 1989
Animal _rat < Mammals
Strain name Fischer
Gender Female
Age at sampling embryo
Tissue embryo/fetus, whole
Classification transformed
Recombinant recombinant
Exogene SV40 (strain SV68C)
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_F822
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 3
User's Publication 2

To topTop
1187  Matsuzaki A, Okuda A, Tamura H, Ohtsu M, Kimura G.  Frequency of cell transformation by the small DNA tumor viruses: infection of proliferating cells and quiescent cells.  Microbiol Immunol  1989  33(8):657-67  PubMed ID: 2550745   DOI: 10.1111/j.1348-0421.1989.tb02016.x
1062  Zaitsu, H., Tanaka, H., Mitsudomi, T., Matsuzaki, A., Ohtsu, M. and Kimura, G.  Differences in proliferation PropertiesAmong Sublines of Rat 3Y1 Fibroblasts Transformed by Various Agents in Vitro  Biomedical Research  1988  9:181-197    DOI: 10.2220/biomedres.9.181
1059  Tanaka H1, Zaitsu H, Onodera K, Kimura G.  Influence of the deprivation of a single amino acid on cellular proliferation and survival in rat 3Y1 fibroblasts and their derivatives transformed by a wide variety of agents.  J Cell Physical  1988  136(3):421-30  PubMed ID: 2844831   DOI: 10.1002/jcp.1041360305

To topTop
User's Publication
2911  Itano, Naoki, Sawai, Takahiro, Atsumi, Fukiko, Miyaishi, Osamu, Taniguchi, Shun'ichiro, Kannagi, Reiji, Hamaguchi, Michinari, Kimata, Koji  Selective expression and functional characteristics of three mammalian hyaluronan synthases in oncogenic malignant transformation.  J Biol Chem  2004  279:18679-87  PubMed ID: 14724275   DOI: 10.1074/jbc.M313178200
17958  Miyagi Y, Sugiyama A, Asai A, Okazaki T, Kuchino Y, Kerr SJ.  Elevated levels of eukaryotic translation initiation factor eIF-4E, mRNA in a broad spectrum of transformed cell lines  Cancer Lett   1995  91(2):247-52  PubMed ID: 7767916   DOI: 10.1016/0304-3835(95)03737-h

Back Back Return Top Page