Cell No. : Cell Name RCB0334 : E1A-3Y1-3  update : 2022/12/20
Comment			A subline of 3Y1 cell line. Transformed by adenovirus type 12 E1A gene.
Comment from the depositor
Terms and conditions			No restriction, but required to cite ref. (Cancer Res 1988;48:578-583, Biomedical Research 1988;9:181-197) and to send a reprint to originator.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Kimura, Genki
		Originator	Kimura, Genki
		Year of deposit	1989
		Animal	_rat < Mammals
		Strain name	Fischer
		Gender	Female
		Age at sampling	embryo
		Tissue	embryo/fetus, whole
		Classification	transformed
		Recombinant	recombinant
		Exogene	pSV2-gpt, E1A
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_H232
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (low glucose) + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP, AST
Reference information		Reference	2
		User's Publication	0

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Reference

1062  Zaitsu, H., Tanaka, H., Mitsudomi, T., Matsuzaki, A., Ohtsu, M. and Kimura, G.  Differences in proliferation PropertiesAmong Sublines of Rat 3Y1 Fibroblasts Transformed by Various Agents in Vitro  Biomedical Research  1988  9:181-197    DOI: 10.2220/biomedres.9.181

1055  Shimura H, Ohtsu M, Matsuzaki A, Mitsudomi T, Onodera K, Kimura G.  Selective cytotoxicity of phospholipids and diacylglycerols to rat 3Y1 fibroblasts transformed by adenovirus type 12 or its E1A gene.  Cancer Res  1988  48(3):578-83  PubMed ID: 2961438

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