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Cell No. : Cell Name
RCB0332 : E1A-3Y1-1  update : 2022/03/11
CommentA subline of 3Y1 cell line. Transformed by adenovirus type 12 E1A gene.
Comment from the depositor
Terms and conditionsNo restriction, but required to cite ref. (Cancer Res 1988;48:578-583) and to send a reprint to originator.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kimura, Genki
Originator Kimura, Genki
Year of deposit 1989
Animal _rat < Mammals
Strain name Fischer
Gender Female
Age at sampling embryo
Tissue embryo/fetus, whole
Classification transformed
Recombinant recombinant
Exogene pSV2-gpt, E1A
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_H226
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 1
User's Publication 3

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1055  Shimura H, Ohtsu M, Matsuzaki A, Mitsudomi T, Onodera K, Kimura G.  Selective cytotoxicity of phospholipids and diacylglycerols to rat 3Y1 fibroblasts transformed by adenovirus type 12 or its E1A gene.  Cancer Res  1988  48(3):578-83  PubMed ID: 2961438  

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User's Publication
14876  Takase S, Kurokawa R, Kondoh Y, Honda K, Suzuki T, Kawahara T, Ikeda H, Dohmae N, Osada H, Shin-Ya K, Kushiro T, Yoshida M, Matsumoto K.  Mechanism of Action of Prethioviridamide, an Anticancer Ribosomally Synthesized and Post-Translationally Modified Peptide with a Polythioamide Structure  ACS Chem Biol   2019  14(8):1819-1828  PubMed ID: 31365229   DOI: 10.1021/acschembio.9b00410
2911  Itano, Naoki, Sawai, Takahiro, Atsumi, Fukiko, Miyaishi, Osamu, Taniguchi, Shun'ichiro, Kannagi, Reiji, Hamaguchi, Michinari, Kimata, Koji  Selective expression and functional characteristics of three mammalian hyaluronan synthases in oncogenic malignant transformation.  J Biol Chem  2004  279:18679-87  PubMed ID: 14724275   DOI: 10.1074/jbc.M313178200
17958  Miyagi Y, Sugiyama A, Asai A, Okazaki T, Kuchino Y, Kerr SJ.  Elevated levels of eukaryotic translation initiation factor eIF-4E, mRNA in a broad spectrum of transformed cell lines  Cancer Lett   1995  91(2):247-52  PubMed ID: 7767916   DOI: 10.1016/0304-3835(95)03737-h

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