Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB0331 : Ad12-3Y1-W5  update : 2022/03/11
CommentA subline of 3Y1 cell line. Transformed by adenovirus type 12.
Comment from the depositor
Terms and conditionsNo restriction, but required to cite ref. (In Vitro 1983;19:376-384) and to send a reprint to originator.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kimura, Genki
Originator Kimura, Genki
Year of deposit 1989
Animal rat < Mammals
Strain name Fischer
Gender Female
Age at sampling embryo
Tissue embryo/fetus, whole
Classification transformed
Recombinant recombinant
Exogene adenovirus type 12
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_H233
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin or pipetting
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 6
User's Publication 0


To topTop
Reference
1187  Matsuzaki A, Okuda A, Tamura H, Ohtsu M, Kimura G.  Frequency of cell transformation by the small DNA tumor viruses: infection of proliferating cells and quiescent cells.  Microbiol Immunol  1989  33(8):657-67  PubMed ID: 2550745
1055  Shimura H, Ohtsu M, Matsuzaki A, Mitsudomi T, Onodera K, Kimura G.  Selective cytotoxicity of phospholipids and diacylglycerols to rat 3Y1 fibroblasts transformed by adenovirus type 12 or its E1A gene.  Cancer Res  1988  48(3):578-83  PubMed ID: 2961438
1046  Ikezaki K, Akiyama S, Miyazaki C, Kimura G, Kuwano M  Imidazole-resistant phenotype and virus transformation in cultured rat cells.  Cancer Res  1984  44:1791-5  PubMed ID: 6713382
1053  Okuda A, Kajiwara Y, Kimura G  Difference in growth factor requirements of rat 3Y1 cells among growth in mass culture, clonal growth in low density culture, and stimulation to enter S phase in resting culture.  In Vitro  1983  19:376-84  PubMed ID: 6190731
1801  Hama S, Kimura G.  Phenotypic characterization of adenovirus type 12 temperature-sensitive mutants in productive infection and transformation.  Microbiol Immunol  1980  24(7):629-47  PubMed ID: 7412597
1803  Yamashita T, Yamamoto K, Shiroki K, Kimura G, Matsui K, Osato T, Shimojo H, Fujinaga K.  Chromosomal alterations of rat cell lines transformed by human adenovirus type-12 virion, whole DNA and left-end DNA fragments.  Int J Cancer  1980  26(4):435-42  PubMed ID: 7251222

To topTop
User's Publication



Back Back Return Top Page