Cell No. : Cell Name
RCB0331 : Ad12-3Y1-W5
update : 2022/03/11
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| Comment | A subline of 3Y1 cell line. Transformed by adenovirus type 12. |
| Comment from the depositor | |
| Terms and conditions | No restriction, but required to cite ref. (In Vitro 1983;19:376-384) and to send a reprint to originator. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Kimura, Genki
|
| Originator |
Kimura, Genki
|
| Year of deposit |
1989
|
| Animal |
_rat
< Mammals
|
| Strain name |
Fischer
|
| Gender |
Female
|
| Age at sampling |
embryo
|
| Tissue |
embryo/fetus, whole
|
| Classification |
transformed
|
| Recombinant |
recombinant
|
| Exogene |
adenovirus type 12
|
| Memo_1 |
Mycoplasma was eliminated with MC-210.
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_H233
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin or pipetting
|
Culture information
|
Passage ratio |
|
1 : 10 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
6
|
| User's Publication |
0
|
| Reference |
|
1187
Matsuzaki A, Okuda A, Tamura H, Ohtsu M, Kimura G.
Frequency of cell transformation by the small DNA tumor viruses: infection of proliferating cells and quiescent cells.
Microbiol Immunol
1989
33(8):657-67
PubMed ID: 2550745
DOI: 10.1111/j.1348-0421.1989.tb02016.x
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1055
Shimura H, Ohtsu M, Matsuzaki A, Mitsudomi T, Onodera K, Kimura G.
Selective cytotoxicity of phospholipids and diacylglycerols to rat 3Y1 fibroblasts transformed by adenovirus type 12 or its E1A gene.
Cancer Res
1988
48(3):578-83
PubMed ID: 2961438
|
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1046
Ikezaki K, Akiyama S, Miyazaki C, Kimura G, Kuwano M
Imidazole-resistant phenotype and virus transformation in cultured rat cells.
Cancer Res
1984
44:1791-5
PubMed ID: 6713382
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1053
Okuda A, Kajiwara Y, Kimura G
Difference in growth factor requirements of rat 3Y1 cells among growth in mass culture, clonal growth in low density culture, and stimulation to enter S phase in resting culture.
In Vitro
1983
19:376-84
PubMed ID: 6190731
DOI: 10.1007/bf02619554
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1801
Hama S, Kimura G.
Phenotypic characterization of adenovirus type 12 temperature-sensitive mutants in productive infection and transformation.
Microbiol Immunol
1980
24(7):629-47
PubMed ID: 7412597
DOI: 10.1111/j.1348-0421.1980.tb02865.x
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1803
Yamashita T, Yamamoto K, Shiroki K, Kimura G, Matsui K, Osato T, Shimojo H, Fujinaga K.
Chromosomal alterations of rat cell lines transformed by human adenovirus type-12 virion, whole DNA and left-end DNA fragments.
Int J Cancer
1980
26(4):435-42
PubMed ID: 7251222
DOI: 10.1002/ijc.2910260408
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