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Cell No. : Cell Name
RCB0328 : Ad12-3Y1-Z11  update : 2022/03/11
CommentA subline of 3Y1 cell line. Transformed by adenovirus type 12.
Comment from the depositor
Terms and conditionsNo restriction, but required to cite ref. (Biomedical Research 1988;9:181-197) and to send a reprint to originator.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Kimura, Genki
Originator Kimura, Genki
Year of deposit 1989
Animal _rat < Mammals
Strain name Fischer
Gender Female
Age at sampling embryo
Tissue embryo/fetus, whole
Classification transformed
Recombinant recombinant
Exogene adenovirus type 12
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_H234
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 5
User's Publication 0

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1187  Matsuzaki A, Okuda A, Tamura H, Ohtsu M, Kimura G.  Frequency of cell transformation by the small DNA tumor viruses: infection of proliferating cells and quiescent cells.  Microbiol Immunol  1989  33(8):657-67  PubMed ID: 2550745   DOI: 10.1111/j.1348-0421.1989.tb02016.x
1062  Zaitsu, H., Tanaka, H., Mitsudomi, T., Matsuzaki, A., Ohtsu, M. and Kimura, G.  Differences in proliferation PropertiesAmong Sublines of Rat 3Y1 Fibroblasts Transformed by Various Agents in Vitro  Biomedical Research  1988  9:181-197    DOI: 10.2220/biomedres.9.181
1055  Shimura H, Ohtsu M, Matsuzaki A, Mitsudomi T, Onodera K, Kimura G.  Selective cytotoxicity of phospholipids and diacylglycerols to rat 3Y1 fibroblasts transformed by adenovirus type 12 or its E1A gene.  Cancer Res  1988  48(3):578-83  PubMed ID: 2961438  
1801  Hama S, Kimura G.  Phenotypic characterization of adenovirus type 12 temperature-sensitive mutants in productive infection and transformation.  Microbiol Immunol  1980  24(7):629-47  PubMed ID: 7412597   DOI: 10.1111/j.1348-0421.1980.tb02865.x
1803  Yamashita T, Yamamoto K, Shiroki K, Kimura G, Matsui K, Osato T, Shimojo H, Fujinaga K.  Chromosomal alterations of rat cell lines transformed by human adenovirus type-12 virion, whole DNA and left-end DNA fragments.  Int J Cancer  1980  26(4):435-42  PubMed ID: 7251222   DOI: 10.1002/ijc.2910260408

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