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Cell No. : Cell Name
RCB0324 : 3Y1-SB-16  update : 2022/03/11
CommentA subline of 3Y1 cell line. Tetraploid clone.
Comment from the depositor
Terms and conditionsNo restriction, but required to cite ref. (In Vitro Cell Dev Biol 1985;21:428-432) and to send a reprint to originator.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kimura, Genki
Originator Kimura, Genki
Year of deposit 1989
Animal rat < Mammals
Strain name Fischer
Gender Female
Age at sampling embryo
Tissue embryo/fetus, whole
Classification semi-normal
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_K106
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST
Reference information Reference 1
User's Publication 0


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Reference
1060  Yamada K, Ohtsu M, Kimura G  Isolation of tetraploid clones with high efficiency from diploid 3Y1 rat fibroblasts treated with sodium butyrate.  In Vitro Cell Dev Biol  1985  21:428-32  PubMed ID: 4030625  

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