Cell No. : Cell Name
RCB0318 : 3Y1ts-202
update : 2020/12/08
|
| Comment | A subline of 3Y1 cell line. Temperature-sensitive mutant. |
| Comment from the depositor | |
| Terms and conditions | No restriction, but required to cite ref. (Somat Cell Mol Genet 1984;10:17-28, J Cell Physiol 1984;119:82-88) and to send a reprint to originator. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
|
Depositor |
Kimura, Genki
|
| Originator |
Kimura, Genki
|
| Year of deposit |
1989
|
| Animal |
rat
< Mammals
|
| Strain name |
Fischer
|
| Gender |
Female
|
| Age at sampling |
embryo
|
| Tissue |
embryo/fetus, whole
|
| Classification |
mutant
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Medium and additives |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% trypsin
|
Culture information
|
Passage ratio |
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1 : 8 split
|
| SC frequency |
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Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
33
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
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| Mycoplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP, AST
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
0
|
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