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Cell No. : Cell Name
RCB0318 : 3Y1ts-202  update : 2018/07/06
CommentTemperature-sensitive mutant of 3Y1 (see library).
Comment from the depositor
Terms and conditionsNo restriction, but required to cite ref. (Somat Cell Mol Genet 1984;10:17-28, J Cell Physiol 1984;119:82-88) and to send a reprint to originator.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kimura, Genki
Originator Kimura, Genki
Year of deposit 1989
Animal rat < Mammals
Strain name Fischer
Gender Female
Age at sampling embryo
Tissue embryo/fetus, whole
Classification mutant
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 33 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
1061  Zaitsu H, Kimura G  Arrest states in a set of mutants of rat 3Y1 cells temperature-sensitive for entering S phase.  J Cell Physiol  1984  119:82-8  PubMed ID: 6707106
1050  Ohno K, Okuda A, Ohtsu M, Kimura G.  Genetic analysis of control of proliferation in fibroblastic cells in culture. I. Isolation and characterization of mutants temperature-sensitive for proliferation or survival of untransformed diploid rat cell line 3Y1.  Somat Cell Mol Genet  1984  10(1):17-28  PubMed ID: 6583851

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