Cell No. : Cell Name
RCB0318 : 3Y1ts-202
update : 2020/12/08
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Comment | A subline of 3Y1 cell line. Temperature-sensitive mutant. |
Comment from the depositor | |
Terms and conditions | No restriction, but required to cite ref. (Somat Cell Mol Genet 1984;10:17-28, J Cell Physiol 1984;119:82-88) and to send a reprint to originator. |
Remarks | |
Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
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Depositor |
Kimura, Genki
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Originator |
Kimura, Genki
|
Year of deposit |
1989
|
Animal |
rat
< Mammals
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Strain name |
Fischer
|
Gender |
Female
|
Age at sampling |
embryo
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Tissue |
embryo/fetus, whole
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Classification |
mutant
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|
Lifespan |
infinite
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Morphology |
fibroblast-like
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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Medium and additives |
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DMEM (low glucose) + 10% FBS
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Antibiotics |
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Free
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Passage method |
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0.25% trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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SC frequency |
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Subculture : once/week, Medium Renewal : 2 times/week
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Temperature |
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33
℃
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CO2 concentration |
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5
%
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Freeze medium |
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Medium + 10% DMSO
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Freezing method |
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Slow freezing
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Mycoplasma |
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(-)
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Animal PCR |
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OK
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Isozyme |
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LD, NP, AST
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Images |
deposit info | lot info |
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Reference information |
Reference |
2
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User's Publication |
0
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