Cell No. : Cell Name
RCB0315 : 3Y1tsG125
update : 2022/03/14
|
| Comment | A subline of 3Y1 cell line. Temperature-sensitive mutant. |
| Comment from the depositor | |
| Terms and conditions | No restriction, but required to cite ref. (Somat Cell Mol Genet 1984;10:17-28) and to send a reprint to originator. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Kimura, Genki
|
| Originator |
Kimura, Genki
|
| Year of deposit |
1989
|
| Animal |
_rat
< Mammals
|
| Strain name |
Fischer
|
| Gender |
Female
|
| Age at sampling |
embryo
|
| Tissue |
embryo/fetus, whole
|
| Classification |
mutant
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_L741
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
33
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
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