Cell No. : Cell Name
RCB0313 : 3Y1tsD123
update : 2024/10/23
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| Comment | A subline of 3Y1 cell line. Temperature-sensitive mutant. |
| Comment from the depositor | |
| Terms and conditions | No restriction, but required to cite ref. (Somat Cell Mol Genet 1984;10:17-28) and to send a reprint to originator. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Kimura, Genki
|
| Originator |
Kimura, Genki
|
| Year of deposit |
1989
|
| Animal |
_rat
< Mammals
|
| Strain name |
Fischer
|
| Gender |
Female
|
| Age at sampling |
embryo
|
| Tissue |
embryo/fetus, whole
|
| Classification |
mutant
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_L735
|
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| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
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1 : 8 split
|
| SC frequency |
|
Subculture : once/week, Medium Renewal : 2 times/week
|
| Temperature |
|
33
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP, MPI
|
| Reference information |
Reference |
4
|
| User's Publication |
0
|
| Reference |
|
2015
Okuda A, Kimura G
An amino acid change in novel protein D123 is responsible for temperature-sensitive G1-phase arrest in a mutant of rat fibroblast line 3Y1.
Exp Cell Res
1996
223:242-9
PubMed ID: 8601400
DOI: 10.1006/excr.1996.0078
|
|
2044
Zaitsu H, Tanaka H, Kimura G
Elongation and shortening of time required for entry into S phase after release from G1 and G0 arrests in temperature-sensitive mutants of rat 3Y1 cells.
Exp Cell Res
1987
170:310-21
PubMed ID: 2439359
DOI: 10.1016/0014-4827(87)90309-0
|
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1061
Zaitsu H, Kimura G
Arrest states in a set of mutants of rat 3Y1 cells temperature-sensitive for entering S phase.
J Cell Physiol
1984
119:82-8
PubMed ID: 6707106
DOI: 10.1002/jcp.1041190114
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1050
Ohno K, Okuda A, Ohtsu M, Kimura G.
Genetic analysis of control of proliferation in fibroblastic cells in culture. I. Isolation and characterization of mutants temperature-sensitive for proliferation or survival of untransformed diploid rat cell line 3Y1.
Somat Cell Mol Genet
1984
10(1):17-28
PubMed ID: 6583851
DOI: 10.1007/bf01534469
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