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Cell No. : Cell Name
RCB0290 : 3Y1-B clone 1  update : 2018/07/06
CommentMother clone of 3Y1 series
Comment from the depositor
Terms and conditionsNo restriction, but required to cite ref. (Int J Cancer 1975;15:694-706) and to send a reprint to originator.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Kimura, Genki
Originator Kimura, Genki
Year of deposit 1989
Original cell 3Y1-B
Animal rat < Mammals
Strain name Fischer
Gender Female
Age at sampling embryo
Tissue embryo/fetus, whole
Metastastatic ability No
Classification semi-normal
Year of origin 1989
Lifespan infinite
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Images
deposit info
lot info
Reference information Reference 2
User's Publication 0


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Reference
1048  Kimura G, Itagaki A, Summers J  Rat cell line 3y1 and its virogenic polyoma- and sv40- transformed derivatives.  Int J Cancer  1975  15:694-706  PubMed ID: 166944
482  Kimura G, Itagaki A.  Initiation and maintenance of cell transformation by simian virus 40: a viral genetic property.  Proc Natl Acad Sci U S A  1975  72(2):673-7  PubMed ID: 164665

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