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Cell No. : Cell Name
RCB0288 : 3Y1-B clone 1-6-2  update : 2022/03/09
CommentSubclone of 3Y1-B clone 1.
Comment from the depositor
Terms and conditionsNo restriction, but required to cite ref. (Int J Cancer 1975;15:694-706) and to send a reprint to originator.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Kimura, Genki
Originator Kimura, Genki
Year of deposit 1989
Original cell 3Y1-B clone 1-6
Animal rat < Mammals
Strain name Fischer
Gender Female
Age at sampling embryo
Tissue embryo/fetus, whole
Classification semi-normal
Year of origin 1970
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_H230
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP, AST
Reference information Reference 3
User's Publication 0


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Reference
1062  Zaitsu, H., Tanaka, H., Mitsudomi, T., Matsuzaki, A., Ohtsu, M. and Kimura, G.  Differences in proliferation PropertiesAmong Sublines of Rat 3Y1 Fibroblasts Transformed by Various Agents in Vitro  Biomedical Research  1988  9:181-197    DOI: 10.2220/biomedres.9.181
1048  Kimura G, Itagaki A, Summers J  Rat cell line 3y1 and its virogenic polyoma- and sv40- transformed derivatives.  Int J Cancer  1975  15:694-706  PubMed ID: 166944   DOI: 10.1002/ijc.2910150419
482  Kimura G, Itagaki A.  Initiation and maintenance of cell transformation by simian virus 40: a viral genetic property.  Proc Natl Acad Sci U S A  1975  72(2):673-7  PubMed ID: 164665   DOI: 10.1073/pnas.72.2.673

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