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Cell No. : Cell Name
RCB0286 : tk^(-)ts13  update : 2022/03/09
CommentThymidine kinase-defective mutant of ts13
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Seto, Toshio
Originator Baserga, R.
Year of deposit 1989
Animal hamster < Mammals
Strain name Syrian
Tissue kidney
Classification mutant
Recombinant recombinant
Exogene simian virus 40 A gene
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_4325
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Chromosome mode 50-151(50) : /70(6),71(4),73(5),74(2),75(6),76(5)/
Reference information Reference 2
User's Publication 0


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Reference
1077  Shen YM, Hirschhorn RR, Mercer WE, Surmacz E, Tsutsui Y, Soprano KJ, Baserga R.  Gene transfer: DNA microinjection compared with DNA transfection with a very high efficiency.  Mol Cell Biol  1982  2(9):1145-54  PubMed ID: 6294505   DOI: 10.1128/mcb.2.9.1145
4670  Talavera A, Basilico C.  Temperature sensitive mutants of BHK cells affected in cell cycle progression.  J Cell Physiol  1977  92(3):425-36  PubMed ID: 903382   DOI: 10.1002/jcp.1040920310

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