Cell No. : Cell Name
RCB0286 : tk^(-)ts13
update : 2025/06/13
|
| Comment | Thymidine kinase-defective mutant of ts13 |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
|
Basic information
|
Depositor |
Seto, Toshio
|
| Originator |
Baserga, R.
|
| Year of deposit |
1989
|
| Animal |
_hamster
< Mammals
|
| Strain name |
Syrian
|
| Tissue |
kidney
|
| Classification |
mutant
|
| Recombinant |
recombinant
|
| Exogene |
simian virus 40 A gene
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_4325
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
DMEM (low glucose) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| DNA barcoding |
|
OK
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
50-151(50) : /70(6),71(4),73(5),74(2),75(6),76(5)/
|
| Reference information |
Reference |
3
|
| User's Publication |
0
|
| Reference |
|
1077
Shen YM, Hirschhorn RR, Mercer WE, Surmacz E, Tsutsui Y, Soprano KJ, Baserga R.
Gene transfer: DNA microinjection compared with DNA transfection with a very high efficiency.
Mol Cell Biol
1982
2(9):1145-54
PubMed ID: 6294505
DOI: 10.1128/mcb.2.9.1145
|
|
4670
Talavera A, Basilico C.
Temperature sensitive mutants of BHK cells affected in cell cycle progression.
J Cell Physiol
1977
92(3):425-36
PubMed ID: 903382
DOI: 10.1002/jcp.1040920310
|
|
18
Macpherson,I. and Stoker,M.
Polyoma transformation of hamster cell clones----an investigation of genetic factors affecting cell competence.
Virology
1962
16:147-151
PubMed ID: 14468055
DOI: 10.1016/0042-6822(62)90290-8
|
Back
Top
CELL BANK Website