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Cell No. : Cell Name
RCB0285 : CHO-K1  update : 2020/09/24
CommentWidely used cell line, especially for biotechnol
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, a prior written permission of the DEPOSITOR or the RIKEN BRC is necessary.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Seto, Toshio
Originator Puck, T. T.
Year of deposit 1989
Animal hamster < Mammals
Strain name Chinese
Genus Cricetulus
Species griseus
Gender Female
Age at sampling adult
Tissue ovary
Classification other
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HamF12 + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Chromosome mode 17-58(50) : 17(3),18(3),19(10),20(21),21(6),26(1)/
deposit info
lot info
Reference information Reference 2
User's Publication 63

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1068  Kao, F T, Puck, T T  Genetics of somatic mammalian cells, VII. Induction and isolation of nutritional mutants in Chinese hamster cells.  Proc Natl Acad Sci U S A  1968  60:1275-81  PubMed ID: 5244736
2586  Puck, T. T. et al  Genetics of somatic mammalian cells. III. Long-term cultivation of euploid cells from human and animal subjects.  J Exp Med  1958  108:945  PubMed ID: 13598821

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User's Publication
6934  Baba K, Kuwada S, Nakao A, Li X, Okuda N, Nishida A, Mitsuda S, Fukuoka N, Kakeya H, Kataoka T.  Different localization of lysosomal-associated membrane protein 1 (LAMP1) in mammalian cultured cell lines.  Histochem Cell Biol  2020    PubMed ID: 31907597
11515  Fukuda N, Senga Y, Honda S.  Anxa2- and Ctsd-knockout CHO cell lines to diminish the risk of contamination with host cell proteins.  Biotechnol. Prog.  2019    PubMed ID: 30972970
11573  Wu A, Grela E, Wójtowicz K, Filipczak N, Hamon Y, Luchowski R, Grudziński W, Raducka-Jaszul O, Gagoś M, Szczepaniak A, Chimini G, Gruszecki WI, Trombik T.  ABCA1 transporter reduces amphotericin B cytotoxicity in mammalian cells.  Cell. Mol. Life Sci.  2019    PubMed ID: 31134303
11673  Hanako Tauchi, Chikahiro Imashiro, Taiki Kuribara, Genichiro Fujii, Yuta Kurashina, Kiichiro Totani, Kenjiro Takemura  Effective and Intact Cell Detachment from a Clinically Ubiquitous Culture Flask by Combining Ultrasonic Wave Exposure and Diluted Trypsin  Biotechnology and Bioprocess Engineering  2019   
6941  Yamamoto S, Iwamaru Y, Shimizu Y, Ueda Y, Sato M, Yamaguchi K, Nakanishi J.  Epidermal growth factor-nanoparticle conjugates change the activity from anti-apoptotic to pro-apoptotic at membrane rafts.  Acta Biomater  2019  88:383-391  PubMed ID: 30794990
11919  Yachi Y, Yoshii Y, Matsuya Y, Mori R, Oikawa J, Date H.  Track Structure Study for Energy Dependency of Electrons and X-rays on DNA Double-Strand Break Induction.  Sci Rep  2019    PubMed ID: 31776470
11172  Nakade S, Mochida K, Kunii A, Nakamae K, Aida T, Tanaka K, Sakamoto N, Sakuma T, Yamamoto T.  Biased genome editing using the local accumulation of DSB repair molecules system.  Nat Commun  2018  9(1):3270  PubMed ID: 30115916
11085  Matsuya Y, McMahon SJ, Tsutsumi K, Sasaki K, Okuyama G, Yoshii Y, Mori R, Oikawa J, Prise KM, Date H.  Investigation of dose-rate effects and cell-cycle distribution under protracted exposure to ionizing radiation for various dose-rates.  Sci Rep  2018  8(1):8287  PubMed ID: 29844494
11155  Yasui M, Hiroshima M, Kozuka J, Sako Y, Ueda M.  Automated single-molecule imaging in living cells.  Nat Commun  2018  9(1):3061  PubMed ID: 30076305
11346  Hishinuma S, Tamura Y, Kobayashi C, Akatsu C, Shoji M.  Differential Regulation of Thermodynamic Binding Forces of Levocetirizine and (S)-Cetirizine by Lys191 in Human Histamine H₁ Receptors.  Int J Mol Sci  2018    PubMed ID: 30558340
4064  Tomoki Ohkubo, Haruyuki Kinoshita, View ORCID ProfileToshiro Maekawa, Katsuyuki Kunida, Hiroshi Kimura, Shinya Kuroda, Teruo Fujii  Development of a microfluidic cell culture and monitoring system for intracellular signaling studies  bioRxiv  2018   
4177  Hishinuma S, Komazaki H, Tsukamoto H, Hatahara H, Fukui H, Shoji M.  Ca2+ -dependent down-regulation of human histamine H1 receptors in Chinese hamster ovary cells.  J. Neurochem.  2018    PubMed ID: 29063596
4474  Hiroshima M, Pack CG, Kaizu K, Takahashi K, Ueda M, Sako Y.  Transient Acceleration of Epidermal Growth Factor Receptor Dynamics Produces Higher-Order Signaling Clusters.  J. Mol. Biol.  2018  430(9):1386-1401  PubMed ID: 29505756
7011  Saito F, Hirayasu K, Satoh T, Wang CW, Lusingu J, Arimori T, Shida K, Palacpac NMQ, Itagaki S, Iwanaga S, Takashima E, Tsuboi T, Kohyama M, Suenaga T, Colonna M, Takagi J, Lavstsen T, Horii T, Arase H.  Immune evasion of Plasmodium falciparum by RIFIN via inhibitory receptors.  Nature  2017  552:101-105  PubMed ID: 29186116
7033  Wakisaka N, Miyasaka N, Koide T, Masuda M, Hiraki-Kajiyama T, Yoshihara Y.  An Adenosine Receptor for Olfaction in Fish.  Curr. Biol.  2017  27:1437-1447.e4  PubMed ID: 28502661
9798  Takagi Y, Yamazaki T, Masuda K, Nishii S, Kawakami B, Omasa T.  Identification of regulatory motifs in the CHO genome for stable monoclonal antibody production.  Cytotechnology  2017  69:451-460  PubMed ID: 27544513
10000  Matsuya Y, Tsutsumi K, Sasaki K, Yoshii Y, Kimura T, Date H.  Modeling cell survival and change in amount of DNA during protracted irradiation.  J. Radiat. Res.  2017  58:302-312  PubMed ID: 27974510
10238  Kinashi Y, Yokomizo N, Takahashi S.  DNA Double-strand Breaks Induced byFractionated Neutron Beam Irradiation for Boron Neutron Capture Therapy.  Anticancer Res.  2017  37:1681-1685  PubMed ID: 28373429
4528  Quazi T.H.Shubhra, Ayako Oyane, Maki Nakamura, Sandra Puentes, Aiki Marushima, HideoTsurushima  Rapid one-pot fabrication of magnetic calcium phosphate nanoparticles immobilizing DNA and iron oxide nanocrystals using injection solutions for magnetofection and magnetic targeting  Materials Today Chemistry  2017  6, 51-61 
9518  Ismail T, Shafi S, Srinivas J, Sarkar D, Qurishi Y, Khazir J, Alam MS, Kumar HM.  Synthesis and tyrosinase inhibition activity of trans-stilbene derivatives.  Bioorg. Chem.  2016  64:97-102  PubMed ID: 26773755
9476  Miki Eto, Hideo Shindou, Takao Shimizu  A Novel Lysophosphatidic Acid Acyltransferase Enzyme (LPAAT4) With a Possible Role for Incorporating Docosahexaenoic Acid Into Brain Glycerophospholipids  Biochem Biophys Res Commun . 2014 Jan 10;443(2):718-24  2014  443(2):718-24  PubMed ID: 24333445
7657  Cao Y, Kimura S, Itoi T, Honda K, Ohtake H, Omasa T.  Construction of BAC-based physical map and analysis of chromosome rearrangement in Chinese hamster ovary cell lines.  Biotechnol. Bioeng.  2012  109:1357-67  PubMed ID: 22012683
5851  Hiragun T, Yanase Y, Kose K, Kawaguchi T, Uchida K, Tanaka S, Hide M.  Surface plasmon resonance-biosensor detects the diversity of responses against epidermal growth factor in various carcinoma cell lines.  Biosens Bioelectron  2012  32(1):202-7  PubMed ID: 22204782
7620  Obayashi H, Kawabe Y, Makitsubo H, Watanabe R, Kameyama Y, Huang S, Takenouchi Y, Ito A, Kamihira M.  Accumulative gene integration into a pre-determined site using Cre/loxP.  J. Biosci. Bioeng.  2011  113:381-8  PubMed ID: 22136795
7629  Cao Y, Kimura S, Itoi T, Honda K, Ohtake H, Omasa T.  Fluorescence in situ hybridization using bacterial artificial chromosome (BAC) clones for the analysis of chromosome rearrangement in Chinese hamster ovary cells.  Methods  2011  56:418-23  PubMed ID: 22100493
7648  Takegawa Y, Araki K, Fujitani N, Furukawa J, Sugiyama H, Sakai H, Shinohara Y.  Simultaneous analysis of heparan sulfate, chondroitin/dermatan sulfates, and hyaluronan disaccharides by glycoblotting-assisted sample preparation followed by single-step zwitter-ionic-hydrophilic interaction chromatography.  Anal. Chem.  2011  83:9443-9  PubMed ID: 22044073
7652  Akasaka Y, Sakai A, Takasu K, Tsukahara M, Hatta A, Suzuki H, Inoue H.  Suppressive effects of glycyrrhetinic acid derivatives on tachykinin receptor activation and hyperalgesia.  J. Pharmacol. Sci.  2011  117:180-8  PubMed ID: 22032861
7734  Hattori K, Sugiura S, Kanamori T.  Scaffold fabrication in a perfusion culture microchamber array chip by O(2) plasma bonding of poly(dimethylsiloxane) protected by a physical mask.  Biomicrofluidics  2011  5:22204  PubMed ID: 21799711
7739  Koike M, Yutoku Y, Koike A.  Accumulation of p21 proteins at DNA damage sites independent of p53 and core NHEJ factors following irradiation.  Biochem. Biophys. Res. Commun.  2011  412:39-43  PubMed ID: 21787748
7852  Yasunaga M, Manabe S, Matsumura Y.  New concept of cytotoxic immunoconjugate therapy targeting cancer-induced fibrin clots.  Cancer Sci.  2011  102:1396-402  PubMed ID: 21481097
7860  Yazaki Y, Oyane A, Sogo Y, Ito A, Yamazaki A, Tsurushima H.  Control of gene transfer on a DNA-fibronectin-apatite composite layer by the incorporation of carbonate and fluoride ions.  Biomaterials  2011  32:4896-902  PubMed ID: 21458066
8217  Matsuzaki K, Harada A, Takeiri A, Tanaka K, Mishima M.  Whole cell-ELISA to measure the gammaH2AX response of six aneugens and eight DNA-damaging chemicals.  Mutat. Res.  2010  700:71-9  PubMed ID: 20580854
8312  Lee BH, Oyane A, Tsurushima H, Shimizu Y, Sasaki T, Koshizaki N.  A new approach for hydroxyapatite coating on polymeric materials using laser-induced precursor formation and subsequent aging.  ACS Appl Mater Interfaces  2010  1:1520-4  PubMed ID: 20355955
8375  Shikano N, Ogura M, Sagara J, Nakajima S, Kobayashi M, Baba T, Yamaguchi N, Iwamura Y, Kubota N, Kawai K.  Stimulation of 125I-3-iodo-alpha-methyl-L-tyrosine uptake in Chinese hamster ovary (CHO-K1) cells by tyrosine esters.  Nucl. Med. Biol.  2010  37:189-96  PubMed ID: 20152718
4679  Takigawa Y, Hata K, Muramatsu S, Amano K, Ono K, Wakabayashi M, Matsuda A, Takada K, Nishimura R, Yoneda T.  The transcription factor Znf219 regulates chondrocyte differentiation by assembling a transcription factory with Sox9.  J Cell Sci  2010  123(Pt 21):3780-8  PubMed ID: 20940257
8467  Nakahara Y, Imanishi S, Mitsumasu K, Kanamori Y, Iwata K, Watanabe M, Kikawada T, Okuda T.  Cells from an anhydrobiotic chironomid survive almost complete desiccation.  Cryobiology  2009  60:138-46  PubMed ID: 19850023
4400  Hoshino T, Namba T, Takehara M, Nakaya T, Sugimoto Y, Araki W, Narumiya S, Suzuki T, Mizushima T.  Prostaglandin E2 stimulates the production of amyloid-beta peptides through internalization of the EP4 receptor.  J Biol Chem.  2009  284(27):18493-502  PubMed ID: 19407341
8792  Shikano N, Nakajima S, Kotani T, Ogura M, Sagara J, Iwamura Y, Yoshimoto M, Kubota N, Ishikawa N, Kawai K.  Transport of D-[1-14C]-amino acids into Chinese hamster ovary (CHO-K1) cells: implications for use of labeled d-amino acids as molecular imaging agents.  Nucl. Med. Biol.  2007  34:659-65  PubMed ID: 17707806
8798  Kikawada T, Saito A, Kanamori Y, Nakahara Y, Iwata K, Tanaka D, Watanabe M, Okuda T.  Trehalose transporter 1, a facilitated and high-capacity trehalose transporter, allows exogenous trehalose uptake into cells.  Proc. Natl. Acad. Sci. U.S.A.  2007  104:11585-90  PubMed ID: 17606922
6288  Yamagata M, Kawano T, Shiba K, Mori T, Katayama Y, Niidome T.  Structural advantage of dendritic poly(L-lysine) for gene delivery into cells.  Bioorg Med Chem  2007  15(1):526-32  PubMed ID: 17035030
3100  Mehnati, Parinaz, Morimoto, Shigeko, Yatagai, Fumio, Furusawa, Yoshiya, Kobayashi, Yasuhiko, Wada, Seiichi, Kanai, Tatsuaki, Hanaoka, Fumio, Sasaki, Hiroshi  Exploration of "over kill effect" of high-LET Ar- and Fe-ions by evaluating the fraction of non-hit cell and interphase death.  J Radiat Res  2005  46:343-350  PubMed ID: 16210791
9019  Omasa T, Kim K, Hiramatsu S, Katakura Y, Kishimoto M, Enosawa S, Ohtake H.  Construction and evaluation of drug-metabolizing cell line for bioartificial liver support system.  Biotechnol. Prog.  2005  21:161-7  PubMed ID: 15903254
3060  Hirukawa, K. Miyazawa, K. Maeda, H. Kameyama, Y. Goto, S. and Togari, A.  Osteogenesis accelerated by CHO-K1 cells transfected with the IGF-I gene  Dentistry in Japan  2004  40:31-34 
3204  Wada, Wataru, Maeshima, Akito, Zhang, You-Qing, Hasegawa, Yoshihisa, Kuwano, Hiroyuki, Kojima, Itaru  Assessment of the function of the betaC-subunit of activin in cultured hepatocytes.  Am J Physiol Endocrinol Metab  2004  287:E247-54  PubMed ID: 15039147
2893  Ichida, Fumitaka, Nishimura, Riko, Hata, Kenji, Matsubara, Takuma, Ikeda, Fumiyo, Hisada, Kunihiro, Yatani, Hirofumi, Cao, Xu, Komori, Toshihisa, Yamaguchi, Akira, Yoneda, Toshiyuki  Reciprocal roles of MSX2 in regulation of osteoblast and adipocyte differentiation.  J Biol Chem  2004  279:34015-22  PubMed ID: 15175325
2701  Tamura M, Natori K, Kobayashi M, Miyamura T, Takeda N  Inhibition of attachment of virions of Norwalk virus to mammalian cells by soluble histone molecules.  Arch Virol  2003  148:1659-70  PubMed ID: 14505080
3032  Wada, Seiichi, Kurahayashi, Hidemitsu, Kobayashi, Yasuhiko, Funayama, Tomoo, Yamamoto, Kazuo, Natsuhori, Masahiro, Ito, Nobuhiko  The relationship between cellular radiosensitivity and radiation-induced DNA damage measured by the comet assay.  J Vet Med Sci  2003  65:471-7  PubMed ID: 12736429
3042  Shimizu, Takeshi, Hamabata, Takashi, Yoshiki, Akemi, Hori, Takeya, Ito, Satoru, Takeda, Yoshifumi, Hayashi, Hideo  An association of 27- and 40-kDa molecules with glycolipids that bind A-B bacterial enterotoxins to cultured cells.  Biochim Biophys Acta  2003  1612:186-94  PubMed ID: 12787937
3031  Wada, Seiichi, Khoa, Tran Van, Kobayashi, Yasuhiko, Funayama, Tomoo, Yamamoto, Kazuo, Natsuhori, Masahiro, Ito, Nobuhiko  Detection of radiation-induced apoptosis using the comet assay.  J Vet Med Sci  2003  65:1161-6  PubMed ID: 14665742
6277  Tatsuya Okuda, Satoru Kidoaki,b Mio Ohsaki, Yoshiyuki Koyama, Kenichi Yoshikawa, Takuro Niidome and Haruhiko Aoyagia   Time-dependent complex formation of dendritic poly(L-lysine) with plasmid DNA and correlation with in vitro transfection efficiencies  Org Biomol Chem  2003  1:1270-1273   PubMed ID: 12929654
9038  Masahiro Nagahama, Shinya Hayashi, Shinsuke Morimitsu, Jun Sakurai  Biological Activities and Pore Formation of Clostridium Perfringens Beta Toxin in HL 60 Cells  J Biol Chem  2003  278 (38), 36934-41  PubMed ID: 12851396
2651  Uchino T, Tokunaga H, Ando M, Utsumi H  Quantitative determination of OH radical generation and its cytotoxicity induced by TiO(2)-UVA treatment.  Toxicol In Vitro  2002  16:629-35  PubMed ID: 12206830
3226  Yamauchi, Yoshio, Abe-Dohmae, Sumiko, Yokoyama, Shinji  Differential regulation of apolipoprotein A-I/ATP binding cassette transporter A1-mediated cholesterol and phospholipid release.  Biochim Biophys Acta  2002  1585:43110  PubMed ID: 12457709
3788  Ohsaki M, Okuda T, Wada A, Hirayama T, Niidome T, Aoyagi H.  In vitro gene transfection using dendritic poly(L-lysine).  Bioconjug Chem  2002  13(3):510-7  PubMed ID: 12009940
2498  Terada M, Shimizu A, Sato N, Miyakaze, S I, Katayama H, Kurokawa-Seo M  Fibroblast growth factor receptor 3 lacking the Ig IIIb and transmembrane domains secreted from humnan aruamous cell carcinoma DJM-1 binds to FGFs.  Mol Cell Biol Res Commun  2001  4(6):365-73  PubMed ID: 11703096
5342  Emoto Y, Kawahara E, Koga Y, Ito T, Tawada K.  Small molecular mass inhibitor of growth of MDCK cells derived from pig spinal cord.  J Cell Physiol  2001  186(3):350-6  PubMed ID: 11169973
6117  Niidome T, Aoyagi H.  Cationic α-Helical Peptides for Gene Delivery into Cells.  Methods Mol Med  2001  65:11-21  PubMed ID: 21318742
2382  Niidome T, Takaji K, Urakawa M, Ohmori N, Wada A, Hirayama T, Aoyagi H  Chain length of cationic alpha-helical peptide sufficient for gene delivery into cells.  Bioconjug Chem  1999  10:773-80  PubMed ID: 10502342
5324  Kiso T, Namikawa T, Tokunaga T, Sawada K, Kakita T, Shogaki T, Ohtsubo Y.  Anti-obesity and anti-diabetic activities of a new beta3 adrenergic receptor agonist, (S)-(Z)-[4-[[1-[2-[(2-hydroxy-3-phenoxypropyl)]amino]ethyl]-1-propenyl] phenoxy] acetic acid ethanedioic acid (SWR-0342SA), in KK-Ay mice.  Biol Pharm Bull  1999  22(10):1073-8  PubMed ID: 10549858
3526  Murakami M, Shimbara S, Kambe T, Kuwata H, Winstead MV, Tischfield JA, Kudo I.  The functions of five distinct mammalian phospholipase A2S in regulating arachidonic acid release. Type IIa and type V secretory phospholipase A2S are functionally redundant and act in concert with cytosolic phospholipase A2.  J Biol Chem  1998  273(23):14411-23  PubMed ID: 9603953
3099  Sasaki H, Yatagai F, Kanai T, Furusawa Y, Hanaoka F, Zhu, W G, Mehnati P  Dependence of induction of interphase death of Chinese hamster ovary cells exposed to accelerated heavy ions on linear energy transfer.  Radiat Res  1997  148:449-54  PubMed ID: 9355870
6248  K. KonomotoB. FitzsimonsJ. ShirokazeK. Sagara  High Speed Stirring Culture Using Macroporous Microcarrier  Animal Cell Technology: Basic & Applied Aspects  1994  447-45 
6250  Junichi Shirokaze et al  Perfusion Culture using a Macroporous Microcarrier  Animal Cell Technology: Basic & Applied Aspects  1994  441-445 

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