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Cell No. : Cell Name
RCB0282 : NFSa Y83  update : 2022/08/12
Comment A subline of NFSa which is a mouse cell line derived from fibrosarcoma. Transplantable.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ando, Koichi
Year of deposit 1989
Animal _mouse < Mammals
Strain name C3H
Tissue fibrosarcoma
Classification cancer
History transferred to in vitro by Yok
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_H735
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP, MPI
Chromosome mode 66-70(50) : /67(4),68(7),69(10),70(12),71(6),72(9)/
Reference information Reference 1
User's Publication 5

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4668  Milas L, Hersh EM, Hunter N.  Therapy of artificial and spontaneous metastases of murine tumors with maleic anhydride-divinyl ether-2.  Cancer Res  1981  41(6):2378-85  PubMed ID: 7237436  

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User's Publication
14340  Fujiwara T, Yakoub MA, Chandler A, Christ AB, Yang G, Ouerfelli O, Rajasekhar VK, Yoshida A, Kondo H, Hata T, Tazawa H, Dogan Y, Moore MAS, Fujiwara T, Ozaki T, Purdue E, Healey JH.  CSF-1/CSF-1R Signaling Inhibitor Pexidartinib (PLX3397) Reprograms Tumor-Associated Macrophages and Stimulates T-Cell Infiltration in the Sarcoma Microenvironment  Mol Cancer Ther  2021    PubMed ID: 34088832   DOI: 10.1158/1535-7163.MCT-20-0591
9095  N Meshii, G Takahashi, S Okunaga, M Hamada, S Iwai, A Takasu, Y Ogawa, Y Yura  Enhancement of Systemic Tumor Immunity for Squamous Cell Carcinoma Cells by an Oncolytic Herpes Simplex Virus  Cancer Gene Ther  2013  20 (9), 493-8  PubMed ID: 23887644   DOI: 10.1038/cgt.2013.45
5371  Fujita M, Hayashi I, Yamashina S, Itoman M, Majima M.  Blockade of angiotensin AT1a receptor signaling reduces tumor growth, angiogenesis, and metastasis.  Biochem Biophys Res Commun  2002  294(2):441-7  PubMed ID: 12051731   DOI: 10.1016/S0006-291X(02)00496-5
17923  Matsumoto K, Saga Y, Ikemura T, Sakakura T, Chiquet-Ehrismann R.  The distribution of tenascin-X is distinct and often reciprocal to that of tenascin-C  J Cell Biol  1994  125(2):483-93  PubMed ID: 7512972   DOI: 10.1083/jcb.125.2.483
17890  Saga Y, Tsukamoto T, Jing N, Kusakabe M, Sakakura T.  Murine tenascin: cDNA cloning, structure and temporal expression of isoforms  Gene  1991  104(2):177-85  PubMed ID: 1717349   DOI: 10.1016/0378-1119(91)90248-a

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