Cell No. : Cell Name
RCB0279 : NIAS-MaBr-92
update : 2024/01/17
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| Comment | Susceptible to nuclear polyhedrosis virus |
| Comment from the depositor | |
| Terms and conditions | No restriction, but the user needs depositor's approval to use for any commercial purpose. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
Mitsuhashi, Jun
|
| Originator |
Mitsuhashi,Jun & Shozawa,Akiko
|
| Year of deposit |
1989
|
| Animal |
_armyworm
< Insect
|
| Strain name |
cabbage armyworm
|
| Genus |
Mamestra
|
| Species |
brassicae
|
| Tissue |
blood, larval
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| Classification |
other
|
| Year of origin |
1982
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|
Lifespan |
infinite
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| Morphology |
other
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| Cellosaurus(Expasy) |
CVCL_Z124
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Suspension cells
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| Culture method |
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浮遊細胞の培養に関する一般的な注意(Japanese)
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| Culture medium |
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MM (or Schneider's) + 3% FBS
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| Antibiotics |
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Free
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|
Passage method |
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dilution
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Culture information
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Passage ratio |
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1 : 8-10 split
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| SC frequency |
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Subculture : 1-2 times/week, Medium Renewal : 2 times/week
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| Temperature |
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23-25
℃
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| CO2 concentration |
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0
%
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| Freeze medium |
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Medium + 10% FBS + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Reference information |
Reference |
5
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| User's Publication |
0
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| Reference |
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1919
Yanagimoto Y, Mitsuhashi J.
Production of rotenone-inactivating substance(s) by rotenone-resistant insect cell line
In Vitro Cell Dev Biol Anim
1996
32(7):399-402
PubMed ID: 8856339
DOI: 10.1007/BF02723001
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1045
Jun MITSUHASHI, Hajime INOUE
Obtainment of a Continuous Cell Line from the Larval Fat Bodies of the Mulberry Tiger Moth, Spilosoma imparilis (Lepidoptera:Arctiidae)
Applied Entomology and Zoology
1988
23:488-490
DOI: 10.1303/aez.23.488
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1092
MITSUHASHI J.
Simplification of media and utilization of sugars by insect cells in cultures.
Invertebrate and Fish Tissue Culture
1988
15-18
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1040
Hajime INOUE, Jun MITSUHASHI
Further Establishment of Continuous Cell Lines from Larval Fat Bodies of the Cabbage Armyworm.
Applied Entomology and Zoology
1985
20:496-498
DOI: 10.1303/aez.20.496
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1043
Mitsuhashi J, Shozawa A.
Continuous Cell Lines from Larval Hemocytes of the Cabbage Armyworm, Mamestra brassiae: (continuous cell line/hemocytes/Mamestra brassicae)
Dev Growth Differ
1985
27(5):599-60
PubMed ID: 37280827
DOI: 10.1111/j.1440-169X.1985.00599.x
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