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Cell No. : Cell Name
RCB0279 : NIAS-MaBr-92  update : 2023/01/04
CommentSusceptible to nuclear polyhedrosis virus
Comment from the depositor
Terms and conditionsNo restriction, but the user needs depositor's approval to use for any commercial purpose.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Mitsuhashi, Jun
Originator Mitsuhashi,Jun & Shozawa,Akiko
Year of deposit 1989
Animal _armyworm < Insect
Strain name cabbage armyworm
Genus Mamestra
Species brassicae
Tissue blood, larval
Classification other
Year of origin 1982
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_Z124
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium MM (or Schneider's) + 3% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8-10 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 23-25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Reference information Reference 5
User's Publication 0

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1919  Yanagimoto Y, Mitsuhashi J.  Production of rotenone-inactivating substance(s) by rotenone-resistant insect cell line  In Vitro Cell Dev Biol Anim  1996  32(7):399-402  PubMed ID: 8856339   DOI: 10.1007/BF02723001
1045  Jun MITSUHASHI, Hajime INOUE  Obtainment of a Continuous Cell Line from the Larval Fat Bodies of the Mulberry Tiger Moth, Spilosoma imparilis (Lepidoptera:Arctiidae)  Applied Entomology and Zoology  1988  23:488-490    DOI: 10.1303/aez.23.488
1092  MITSUHASHI J.  Simplification of media and utilization of sugars by insect cells in cultures.  Invertebrate and Fish Tissue Culture  1988  15-18   
1040  Hajime INOUE, Jun MITSUHASHI  Further Establishment of Continuous Cell Lines from Larval Fat Bodies of the Cabbage Armyworm.  Applied Entomology and Zoology  1985  20:496-498    DOI: 10.1303/aez.20.496
1043  Mitsuhashi J, Shozawa A.  Continuous Cell Lines from Larval Hemocytes of the Cabbage Armyworm, Mamestra brassiae: (continuous cell line/hemocytes/Mamestra brassicae)  Dev Growth Differ  1985  27(5):599-60  PubMed ID: 37280827   DOI: 10.1111/j.1440-169X.1985.00599.x

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