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Cell No. : Cell Name
RCB0279 : NIAS-MaBr-92  update : 2022/03/09
CommentSusceptible to nuclear polyhedrosis virus
Comment from the depositor
Terms and conditionsNo restriction, but the user needs depositor's approval to use for any commercial purpose.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Mitsuhashi, Jun
Originator Mitsuhashi,Jun & Shozawa,Akiko
Year of deposit 1989
Animal armyworm < Insect
Strain name cabbage armyworm
Genus Mamestra
Species brassicae
Tissue blood, larval
Classification other
Year of origin 1982
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_Z124
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives MM (or Schneider's) + 3% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 8-10 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 23-25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Reference information Reference 2
User's Publication 0


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Reference
1919  Yanagimoto Y, Mitsuhashi J.  Production of rotenone-inactivating substance(s) by rotenone-resistant insect cell line  In Vitro Cell Dev Biol Anim  1996  32(7):399-402  PubMed ID: 8856339
1040  Hajime INOUE, Jun MITSUHASHI  Further Establishment of Continuous Cell Lines from Larval Fat Bodies of the Cabbage Armyworm.  Applied Entomology and Zoology  1985  20:496-498 

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