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Cell No. : Cell Name
RCB0278 : NIAS-MB-25  update : 2024/01/17
CommentShow finely branched cytoplasmic processes
Comment from the depositor
Terms and conditionsNo restriction, but the user needs depositor's approval to use for any commercial purpose.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Mitsuhashi, Jun
Originator Mitsuhashi, Jun
Year of deposit 1989
Animal _armyworm < Insect
Strain name cabbage armyworm
Genus Mamestra
Species brassicae
Tissue pupal ovary
Classification other
Year of origin 1974
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_Z122
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium MM (or Schneider's) + 3% FBS
Culture method
Antibiotics Free
Passage method pipetting
Culture information Passage cell No 1 x 10 5 cells/ml
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 25 ℃
CO2 concentration 0 %
Freeze medium Medium + 10% FBS + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Reference information Reference 2
User's Publication 0


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Reference
1092  MITSUHASHI J.  Simplification of media and utilization of sugars by insect cells in cultures.  Invertebrate and Fish Tissue Culture  1988  15-18   
1042  Mitsuhashi J.  ESTABLISHMENT AND CHARACTERIZATION OF CONTINUOUS CELL LINES FROM PUPAL OVARIES OF THE CABBAGE ARMYWORM, MAMESTRA BRASSICAE (LEPIDOPTERA, NOCTUIDAE).  Dev Growth Differ  1977  19(4):337-344  PubMed ID: 37281049  

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