細胞番号 : 細胞名
RCB0273 : BRL
update : 2024/02/19
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細胞特性(Comment:英) | ES cell differentiation inhibitory activity (DIA) |
細胞特性(日) | 胎児性未分化幹細胞の分化抑制因子(DIA)を産生するバッファローラット肝細胞。 |
細胞特性(寄託者記述:英) | |
細胞特性(寄託者記述:日) | |
使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
備考(英) | |
備考(日) | |
提供申込書類(英) |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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提供申込書類(日) |
依頼書C-0001.pdf
 
同意書(非営利学術目的)C-0003.pdf
 
同意書(営利目的)C-0003p.pdf
 
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
細胞基本情報
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寄託者 |
Aizawa, Shinichi
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寄託日 |
1989
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動物種 |
_rat
< Mammals
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動物系統名 |
Buffalo
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採取組織 |
liver
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細胞分類 |
other
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入手歴 |
Hooper, M. - Aizawa, S.
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細胞寿命 |
infinite
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細胞形態 |
epithelial-like
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Cellosaurus(Expasy) |
CVCL_4565
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細胞培養・検査情報
| |
寄託時情報 |
ロット情報 |
培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent cells
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培地 |
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DMEM (low glucose) + 10% FBS
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抗生物質 |
|
Free
|
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継代方法 |
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0.25% Trypsin
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継代密度 |
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1 : 8 split
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継代・培地交換頻度 |
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Subculture : 2 times/week
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培養最適温度 |
|
37
℃
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二酸化炭素濃度 |
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5
%
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凍結培地 |
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Medium + 10% DMSO
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凍結方法 |
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Slow freezing
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マイコプラズマ/アコレプラズマ |
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(-)
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動物種PCR |
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OK
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アイソザイム検査 |
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LD, NP
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染色体検査 |
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39-81(50) : 39(4),40(8),41(25),42(12),81(1)
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文献情報 |
Reference(英) |
3件
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Reference(日) |
0件
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利用者成果(英) |
7件
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利用者成果(日) |
0件
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Reference(英) |
1096
Thompson S, Clarke, A R, Pow, A M, Hooper, M L, Melton, D W
Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells.
Cell
1989
56:313-21
PubMed ID: 2912572
DOI: 10.1016/0092-8674(89)90905-7
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1095
Smith, A G, Heath, J K, Donaldson, D D, Wong, G G, Moreau J, Stahl M, Rogers D
Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides.
Nature
1988
336:688-90
PubMed ID: 3143917
DOI: 10.1038/336688a0
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4667
Smith AG, Hooper ML.
Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells.
Dev Biol
1987
121(1):1-9
PubMed ID: 3569655
DOI: 10.1016/0012-1606(87)90132-1
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利用者成果(英) |
8660
Kobayashi M, Terawaki Y, Saito K, Kasuga K, Kojima I.
Effect of medium conditioned with rat hepatoma BRL cells on '2-cell block' of random-bred mouse embryos cultured in vitro.
Zygote
2009
17:169-74
PubMed ID: 19187567
DOI: 10.1017/S0967199408005121
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9049
Hironori Okada, Masao Ito, Yoshihiro Hirose, Akihiko Uda, Keiji Terao, Takashi Yoshida, Tadashi Sankai
Buffalo Rat Liver Cells Produce Factors That Support Preimplantation Development of Mouse Embryos Cultured in Vitro
Comp Med
2005
55 (1), 61-6
PubMed ID: 15766211
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19028
Osano K, Otsuka A, Ono M.
State of chromatin sensitivity to DNase I in the rat Ig-beta/growth hormone locus determined by real-time PCR
Biol Pharm Bull
2004
27(2):222-5
PubMed ID: 14758038
DOI: 10.1248/bpb.27.222
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18034
Kazahari K, Sano E, Matsuura N, Ono M.
Chromatin structure of the rat somatotropin gene locus and physical linkage of the rat somatotropin gene and skeletal-muscle sodium-channel gene
Eur J Biochem
1997
244(2):494-500
PubMed ID: 9119017
DOI: 10.1111/j.1432-1033.1997.00494.x
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17949
Kageyama Y, Yamazaki Y, Afify AS, Ogawa Y, Okada T, Okuno H.
Stereoselective hydrolysis of xenobiotic esters by different cell lines from rat liver and hepatoma and its application to chiral prodrugs for designated growth suppression of cancer cells
Chirality
1995
7(4):297-304
PubMed ID: 7640174
DOI: 10.1002/chir.530070418
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1072
Ya-Li Hsu, Yin-Hsiu Yang, Yu-Chin Chen, Ming-Chen Tung, Jen-Leih Wu, Mark H. Engelking, Joann C. Leong
Development of an in vitro subculture system for the oka organ (Lymphoid tissue) of Penaeus monodon
Aquaculture
1995
136:43-55
DOI: 10.1016/0044-8486(95)01048-3
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1633
Maeda H, Kukita T, Akamine A, Kukita A, Iijima T.
Localization of osteopontin in resorption lacunae formed by osteoclast-like cells: a study by a novel monoclonal antibody which recognizes rat osteopontin
Histochemistry
1994
102(4):247-54
PubMed ID: 7843987
DOI: 10.1007/BF00269160
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