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Cell No. : Cell Name
RCB0273 : BRL  update : 2021/05/20
CommentES cell differentiation inhibitory activity (DIA)
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Aizawa, Shinichi
Year of deposit 1989
Animal _rat < Mammals
Strain name Buffalo
Tissue liver
Classification other
History Hooper, M. - Aizawa, S.
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_4565
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Chromosome mode 39-81(50) : 39(4),40(8),41(25),42(12),81(1)
Reference information Reference 3
User's Publication 2

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1096  Thompson S, Clarke, A R, Pow, A M, Hooper, M L, Melton, D W  Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells.  Cell  1989  56:313-21  PubMed ID: 2912572
1095  Smith, A G, Heath, J K, Donaldson, D D, Wong, G G, Moreau J, Stahl M, Rogers D  Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides.  Nature  1988  336:688-90  PubMed ID: 3143917
4667  Smith AG, Hooper ML.  Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells.  Dev Biol  1987  121(1):1-9  PubMed ID: 3569655

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User's Publication
8660  Kobayashi M, Terawaki Y, Saito K, Kasuga K, Kojima I.  Effect of medium conditioned with rat hepatoma BRL cells on '2-cell block' of random-bred mouse embryos cultured in vitro.  Zygote  2009  17:169-74  PubMed ID: 19187567
9049  Hironori Okada, Masao Ito, Yoshihiro Hirose, Akihiko Uda, Keiji Terao, Takashi Yoshida, Tadashi Sankai  Buffalo Rat Liver Cells Produce Factors That Support Preimplantation Development of Mouse Embryos Cultured in Vitro  Comp Med  2005  55 (1), 61-6  PubMed ID: 15766211

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