Cell No. : Cell Name
RCB0273 : BRL
update : 2024/02/19
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Comment | ES cell differentiation inhibitory activity (DIA) |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Aizawa, Shinichi
|
Year of deposit |
1989
|
Animal |
_rat
< Mammals
|
Strain name |
Buffalo
|
Tissue |
liver
|
Classification |
other
|
History |
Hooper, M. - Aizawa, S.
|
|
Lifespan |
infinite
|
Morphology |
epithelial-like
|
Cellosaurus(Expasy) |
CVCL_4565
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
Culture medium |
|
DMEM (low glucose) + 10% FBS
|
Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
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Culture information
|
Passage ratio |
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1 : 8 split
|
SC frequency |
|
Subculture : 2 times/week
|
Temperature |
|
37
℃
|
CO2 concentration |
|
5
%
|
Freeze medium |
|
Medium + 10% DMSO
|
Freezing method |
|
Slow freezing
|
Mycoplasma/Acholeplasma |
|
(-)
|
Animal PCR |
|
OK
|
Isozyme |
|
LD, NP
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Chromosome mode |
|
39-81(50) : 39(4),40(8),41(25),42(12),81(1)
|
Reference information |
Reference |
3
|
User's Publication |
7
|
Reference |
1096
Thompson S, Clarke, A R, Pow, A M, Hooper, M L, Melton, D W
Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells.
Cell
1989
56:313-21
PubMed ID: 2912572
DOI: 10.1016/0092-8674(89)90905-7
|
1095
Smith, A G, Heath, J K, Donaldson, D D, Wong, G G, Moreau J, Stahl M, Rogers D
Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides.
Nature
1988
336:688-90
PubMed ID: 3143917
DOI: 10.1038/336688a0
|
4667
Smith AG, Hooper ML.
Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells.
Dev Biol
1987
121(1):1-9
PubMed ID: 3569655
DOI: 10.1016/0012-1606(87)90132-1
|
User's Publication |
8660
Kobayashi M, Terawaki Y, Saito K, Kasuga K, Kojima I.
Effect of medium conditioned with rat hepatoma BRL cells on '2-cell block' of random-bred mouse embryos cultured in vitro.
Zygote
2009
17:169-74
PubMed ID: 19187567
DOI: 10.1017/S0967199408005121
|
9049
Hironori Okada, Masao Ito, Yoshihiro Hirose, Akihiko Uda, Keiji Terao, Takashi Yoshida, Tadashi Sankai
Buffalo Rat Liver Cells Produce Factors That Support Preimplantation Development of Mouse Embryos Cultured in Vitro
Comp Med
2005
55 (1), 61-6
PubMed ID: 15766211
|
19028
Osano K, Otsuka A, Ono M.
State of chromatin sensitivity to DNase I in the rat Ig-beta/growth hormone locus determined by real-time PCR
Biol Pharm Bull
2004
27(2):222-5
PubMed ID: 14758038
DOI: 10.1248/bpb.27.222
|
18034
Kazahari K, Sano E, Matsuura N, Ono M.
Chromatin structure of the rat somatotropin gene locus and physical linkage of the rat somatotropin gene and skeletal-muscle sodium-channel gene
Eur J Biochem
1997
244(2):494-500
PubMed ID: 9119017
DOI: 10.1111/j.1432-1033.1997.00494.x
|
17949
Kageyama Y, Yamazaki Y, Afify AS, Ogawa Y, Okada T, Okuno H.
Stereoselective hydrolysis of xenobiotic esters by different cell lines from rat liver and hepatoma and its application to chiral prodrugs for designated growth suppression of cancer cells
Chirality
1995
7(4):297-304
PubMed ID: 7640174
DOI: 10.1002/chir.530070418
|
1072
Ya-Li Hsu, Yin-Hsiu Yang, Yu-Chin Chen, Ming-Chen Tung, Jen-Leih Wu, Mark H. Engelking, Joann C. Leong
Development of an in vitro subculture system for the oka organ (Lymphoid tissue) of Penaeus monodon
Aquaculture
1995
136:43-55
DOI: 10.1016/0044-8486(95)01048-3
|
1633
Maeda H, Kukita T, Akamine A, Kukita A, Iijima T.
Localization of osteopontin in resorption lacunae formed by osteoclast-like cells: a study by a novel monoclonal antibody which recognizes rat osteopontin
Histochemistry
1994
102(4):247-54
PubMed ID: 7843987
DOI: 10.1007/BF00269160
|