Cell No. : Cell Name
							 RCB0273 : BRL
						
						 update : 2024/02/19
						
						 | 
					
						
						
						
							
								| Comment | ES cell differentiation inhibitory activity (DIA) | 
							
							
								| Comment from the depositor |  | 
							
							
								| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | 
							
							
								| Remarks |  | 
							
							
							
								| Order Form | 
								
									
										
											
										
										Order Form(C-0005.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007.pdf)
										
											
										
										 
									
										
											
										
										MTA(C-0007p.pdf)
										
											
										
										 
									
								 | 
							
							
								| 
									Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
								 | 
							
						
						
					
						
						
							
							| 
							
							
							
								
							
							
							 | 
						
						
						
						
					
						
						
						
							
							
							
							
								Basic information
							
							
							 | 
						
						
						
							
								
									
										
										
										
										
				
                                
                                
                                
                                        Depositor | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								Aizawa, Shinichi
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Year of deposit | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								1989
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Animal | 
                                
                                
				
				
				
					
						
						
							
								 
								
								 	_rat
								
								
								
							
								 
								
								
								 	
								 		< Mammals
								 	
								
								
							
						
						
						
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Strain name | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								Buffalo
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Tissue | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								liver
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Classification | 
                                
                                
				
				
				
					
						
						
						
							other
						
						
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | History | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								Hooper, M. - Aizawa, S.
							
							
						
						
							
						
					 | 
				
				
					
					
									
								
									
								
									
								
									
								
							
						
					
						
						
						
							
							
							
							
								
							
							
							 | 
						
						
						
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        Lifespan | 
                                
                                
				
				
				
					
						
						
						
							infinite
						
						
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Morphology | 
                                
                                
				
				
				
					
						
						
						
							epithelial-like
						
						
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
								
							
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
								
									
										
										
										
										
				
                                
                                
                                
                                        | Cellosaurus(Expasy) | 
                                
                                
				
				
				
					
						
						
						
							
						
						
						
							
							
							
								CVCL_4565
							
							
						
						
							
						
					 | 
				
				
					
					
									
								
							
						
					
					
					
					
					
					
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
								
							
						
					
					
						
						
							
							
								| 
									
										
										
											
											
											 |  | 
											deposit info | 
											lot info | 
											
												 | 
												Medium | 
												Medium List | 
											
										
									
								
						
						
						
						
						
					
						
						
						
						
							
							
							
							
								
							
							
							 | 
						
						
						
							
								
									
									
										
										
										
										
				
				
				
				
					Culture type | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							Adherent cells
						
						
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Culture medium | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								DMEM (low glucose) + 10% FBS
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Antibiotics | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								Free
							
							
						
						
							
						
					 | 
				
				
					
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
						
					
						
						
						
						
							
							
							
							
								
							
							
							 | 
						
						
						
							
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					Passage method | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								0.25% Trypsin
							
							
						
						
							
						
					 | 
				
				
					
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
						
					
						
						
						
						
							
							
							
							
								Culture information
							
							
							 | 
						
						
						
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					Passage ratio | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								1 : 8 split
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| SC frequency | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								Subculture : 2 times/week
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Temperature | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								37
							
							
						
						
							℃
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| CO2 concentration | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								5
							
							
						
						
							%
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Freeze medium | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								Medium + 10% DMSO
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Freezing method | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							Slow freezing
						
						
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Mycoplasma/Acholeplasma | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							(-)
						
						
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Animal PCR | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								OK
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Isozyme | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								LD, NP
							
							
						
						
							
						
					 | 
				
				
					
					
						
					
									
								
									
									
								
							
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
								
									
									
										
										
										
										
				
				
				
				
					| Chromosome mode | 
				
				
				
				
				
				
					
					
						
						
						
							
						
						
						
						
					
					 | 
					
					
						
						
						
							
						
						
						
							
							
							
								39-81(50) : 39(4),40(8),41(25),42(12),81(1)
							
							
						
						
							
						
					 | 
				
				
					
					
									
								
							
						
					
					
					
					
					
			
			
			
			
					
			
					
			
						
						
						
							
								| Reference information | 
								Reference | 
									
									
									
										3
									
									
								 | 
							
							
								| User's Publication | 
									
									
									
										7
									
									
								 | 
							
						
						
				
			
				
				
				
				
				
					
						| Reference | 
						
							
							| 
								
								
								
								1096 
								Thompson S, Clarke, A R, Pow, A M, Hooper, M L, Melton, D W 
								Germ line transmission and expression of a corrected HPRT gene produced by gene targeting in embryonic stem cells. 
								Cell 
								1989 
								56:313-21 
								
									
										PubMed ID: 2912572
									
								 
								
									
										DOI: 10.1016/0092-8674(89)90905-7
									
								
							 | 
							
						
							
							| 
								
								
								
								1095 
								Smith, A G, Heath, J K, Donaldson, D D, Wong, G G, Moreau J, Stahl M, Rogers D 
								Inhibition of pluripotential embryonic stem cell differentiation by purified polypeptides. 
								Nature 
								1988 
								336:688-90 
								
									
										PubMed ID: 3143917
									
								 
								
									
										DOI: 10.1038/336688a0
									
								
							 | 
							
						
							
							| 
								
								
								
								4667 
								Smith AG, Hooper ML. 
								Buffalo rat liver cells produce a diffusible activity which inhibits the differentiation of murine embryonal carcinoma and embryonic stem cells. 
								Dev Biol 
								1987 
								121(1):1-9 
								
									
										PubMed ID: 3569655
									
								 
								
									
										DOI: 10.1016/0012-1606(87)90132-1
									
								
							 | 
							
						
					
				
					
						| User's Publication | 
						
							
							| 
								
								
								8660 
								Kobayashi M, Terawaki Y, Saito K, Kasuga K, Kojima I. 
								Effect of medium conditioned with rat hepatoma BRL cells on '2-cell block' of random-bred mouse embryos cultured in vitro. 
								Zygote 
								2009 
								17:169-74 
								
									
										PubMed ID: 19187567
									
								 
								
									
										DOI: 10.1017/S0967199408005121
									
								
							 | 
							
						
							
							| 
								
								
								9049 
								Hironori Okada, Masao Ito, Yoshihiro Hirose, Akihiko Uda, Keiji Terao, Takashi Yoshida, Tadashi Sankai 
								Buffalo Rat Liver Cells Produce Factors That Support Preimplantation Development of Mouse Embryos Cultured in Vitro 
								Comp Med 
								2005 
								55 (1), 61-6 
								
									
										PubMed ID: 15766211
									
								 
								
							 | 
							
						
							
							| 
								
								
								19028 
								Osano K, Otsuka A, Ono M. 
								State of chromatin sensitivity to DNase I in the rat Ig-beta/growth hormone locus determined by real-time PCR 
								Biol Pharm Bull 
								2004 
								27(2):222-5 
								
									
										PubMed ID: 14758038
									
								 
								
									
										DOI: 10.1248/bpb.27.222
									
								
							 | 
							
						
							
							| 
								
								
								18034 
								Kazahari K, Sano E, Matsuura N, Ono M. 
								Chromatin structure of the rat somatotropin gene locus and physical linkage of the rat somatotropin gene and skeletal-muscle sodium-channel gene 
								Eur J Biochem 
								1997 
								244(2):494-500 
								
									
										PubMed ID: 9119017
									
								 
								
									
										DOI: 10.1111/j.1432-1033.1997.00494.x
									
								
							 | 
							
						
							
							| 
								
								
								17949 
								Kageyama Y, Yamazaki Y, Afify AS, Ogawa Y, Okada T, Okuno H. 
								Stereoselective hydrolysis of xenobiotic esters by different cell lines from rat liver and hepatoma and its application to chiral prodrugs for designated growth suppression of cancer cells 
								Chirality 
								1995 
								7(4):297-304 
								
									
										PubMed ID: 7640174
									
								 
								
									
										DOI: 10.1002/chir.530070418
									
								
							 | 
							
						
							
							| 
								
								
								1072 
								Ya-Li Hsu, Yin-Hsiu Yang, Yu-Chin Chen, Ming-Chen Tung, Jen-Leih Wu, Mark H. Engelking, Joann C. Leong 
								Development of an in vitro subculture system for the oka organ (Lymphoid tissue) of Penaeus monodon 
								Aquaculture 
								1995 
								136:43-55 
								 
								
									
										DOI: 10.1016/0044-8486(95)01048-3
									
								
							 | 
							
						
							
							| 
								
								
								1633 
								Maeda H, Kukita T, Akamine A, Kukita A, Iijima T. 
								Localization of osteopontin in resorption lacunae formed by osteoclast-like cells: a study by a novel monoclonal antibody which recognizes rat osteopontin 
								Histochemistry 
								1994 
								102(4):247-54 
								
									
										PubMed ID: 7843987
									
								 
								
									
										DOI: 10.1007/BF00269160
									
								
							 |