細胞番号 : 細胞名
RCB0271 : HeLa S3 (SC)
update : 2023/10/30
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| 細胞特性(Comment:英) | Suspension culturable. Useful for JIS medium inspection |
| 細胞特性(日) | HeLaの亜株の一つ。浮遊培養も可能な付着性培養株。この株はJIS規格のMEM培地検定用細胞株として使用できる。浮遊培養を行う場合は培地をMEM(suspension)に変更する。 |
| 細胞特性(寄託者記述:英) | |
| 細胞特性(寄託者記述:日) | |
| 使用条件(英) | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| 使用条件(日) | 基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。 |
| 備考(英) | |
| 備考(日) | |
| 提供申込書類(英) |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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| 提供申込書類(日) |
依頼書C-0001.pdf
同意書(非営利学術目的)C-0003.pdf
同意書(営利目的)C-0003p.pdf
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提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
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| 提供手数料 |
手数料とお支払いについてはこちらをご覧ください。 |
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細胞基本情報
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寄託者 |
Umeda, Makoto
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| 樹立者 |
Gey, G. O.
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| 寄託日 |
1988
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| 動物種 |
_human
< Mammals
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| 属名 |
Homo
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| 種名 |
sapiens
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| 性別 |
Female
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| 採取組織 |
cervix
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| 病名 |
cervical carcinoma
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| 細胞分類 |
cancer
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| 入手歴 |
JCRB
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|
細胞寿命 |
infinite
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| 細胞形態 |
epithelial-like
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| Cellosaurus(Expasy) |
CVCL_2934
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細胞培養・検査情報
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寄託時情報 |
ロット情報 |
| 培地・試薬情報 |
培地・試薬一覧はこちらをご覧ください。 |
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培養形態 |
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Adherent/Suspension cells
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| 培地 |
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MEM + 10% CS
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| 抗生物質 |
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Free
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継代方法 |
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0.25% Trypsin
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|
継代密度 |
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1 : 8 split
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| 継代・培地交換頻度 |
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Subculture : 1-2 times/week, Medium Renewal : 2 times/week
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| 培養最適温度 |
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37
℃
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| 二酸化炭素濃度 |
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5
%
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| 凍結培地 |
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Medium + 10% DMSO
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| 凍結方法 |
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Slow freezing
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| マイコプラズマ/アコレプラズマ |
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(-)
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| アイソザイム検査 |
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LD, NP
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| 染色体検査 |
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59-72(50) : /65(4),66(12),67(5),68(7),69(7),70(4)/
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| 個体識別検査 |
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OK
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| 画像情報 |
寄託時情報 | ロット情報 |
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| 文献情報 |
Reference(英) |
8件
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| Reference(日) |
2件
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| 利用者成果(英) |
13件
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| 利用者成果(日) |
0件
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| Reference(英) |
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207
Prescott,D.M. (ed.)
Methods Cell Biol
1973
4:1-42
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350
Kuroki T.
Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.
Exp Cell Res
1973
80(1):55-62
PubMed ID: 4361348
DOI: 10.1016/0014-4827(73)90274-7
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154
Hanaoka F, Yamada M
Localization of the replication point of mammalian cell DNA at the membrane.
Biochem Biophys Res Commun
1971
42:647-53
PubMed ID: 5543947
DOI: 10.1016/0006-291x(71)90537-7
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151
GOLDSTEIN, M N, SLOTNICK, I J, JOURNEY, L J
In vitro studies with HeLa cell line sensitive and resistant to actinomycin D.
Ann N Y Acad Sci
1960
89:474-83
PubMed ID: 13706673
DOI: 10.1111/j.1749-6632.1960.tb20171.x
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1446
J E DARNELL Jr, T K SAWYERc
Variation in plaque-forming ability among parental and clonal strains of HeLa cells
Virology
1959
223-9
PubMed ID: 13669339
DOI: 10.1016/0042-6822(59)90006-6
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1445
SATO G, FISHER HW, PUCK TT.
Molecular growth requirements of single mammalian cells.
Science
1957
126(3280):461-4
PubMed ID: 13486039
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206
PUCK TT, MARCUS PI, CIECIURA SJ.
Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.
J Exp Med
1956
103(2):273-83
PubMed ID: 13286432
DOI: 10.1084/jem.103.2.273
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150
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res
1952
12:264-265
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| 利用者成果(英) |
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16658
Sasaki K, Suzuki M, Sonoda T, Schneider-Poetsch T, Ito A, Takagi M, Fujishiro S, Sohtome Y, Dodo K, Umehara T, Aburatani H, Shin-Ya K, Nakao Y, Sodeoka M, Yoshida M.
Visualization of the dynamic interaction between nucleosomal histone H3K9 tri-methylation and HP1α chromodomain in living cells
Cell Chem Biol
2022
S2451-9456(22)00198-2
PubMed ID: 35728598
DOI: 10.1016/j.chembiol.2022.05.006
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6924
Yu Nakagawa et al
Relationship Among Structure, Cytotoxicity, and Michael Acceptor Reactivity of Quinocidin
Bioorganic & Medicinal Chemistry
2020
28(4):115308
PubMed ID: 31956051
DOI: 10.1016/j.bmc.2020.115308
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9512
Sun Y, Tomura T, Sato J, Iizuka T, Fudou R, Ojika M.
Isolation and Biosynthetic Analysis of Haliamide, a New PKS-NRPS Hybrid Metabolite from the Marine Myxobacterium Haliangium ochraceum.
Molecules
2016
21:59
PubMed ID: 26751435
DOI: 10.3390/molecules21010059
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6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
DOI: 10.1111/j.1749-0774.2009.00071.x
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3165
Takamatsu, Shinji, Furukawa, Takako, Mori, Tetsuya, Yonekura, Yoshiharu, Fujibayashi, Yasuhisa
Noninvasive imaging of transplanted living functional cells transfected with a reporter estrogen receptor gene.
Nucl Med Biol
2005
32:821-9
PubMed ID: 16253806
DOI: 10.1016/j.nucmedbio.2005.06.010
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3164
Takamatsu, Shinji, Inoue, Noboru, Katsumata, Toshiyuki, Nakamura, Katsumi, Fujibayashi, Yasuhisa, Takeuchi, Makoto
The relationship between the branch-forming glycosyltransferases and cell surface sugar chain structures.
Biochemistry
2005
44:6343-9
PubMed ID: 15835923
DOI: 10.1021/bi047606a
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6281
Okuda T, Sugiyama A, Niidome T, Aoyagi H.
Characters of dendritic poly(L-lysine) analogues with the terminal lysines replaced with arginines and histidines as gene carriers in vitro.
Biomaterials
2004
25(3):537-44
PubMed ID: 14585703
DOI: 10.1016/s0142-9612(03)00542-8
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3788
Ohsaki M, Okuda T, Wada A, Hirayama T, Niidome T, Aoyagi H.
In vitro gene transfection using dendritic poly(L-lysine).
Bioconjug Chem
2002
13(3):510-7
PubMed ID: 12009940
DOI: 10.1021/bc015525a
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6117
Niidome T, Aoyagi H.
Cationic α-Helical Peptides for Gene Delivery into Cells.
Methods Mol Med
2001
65:11-21
PubMed ID: 21318742
DOI: 10.1385/1-59259-139-6:11
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2382
Niidome T, Takaji K, Urakawa M, Ohmori N, Wada A, Hirayama T, Aoyagi H
Chain length of cationic alpha-helical peptide sufficient for gene delivery into cells.
Bioconjug Chem
1999
10:773-80
PubMed ID: 10502342
DOI: 10.1021/bc990012d
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18039
Nakagawa S, Okaya Y, Yatsunami K, Tanaka S, Ohtsu H, Fukui T, Watanabe T, Ichikawa A.
Identification of multiple regulatory elements of human L-histidine decarboxylase gene
J Biochem
1997
121(5):935-40
PubMed ID: 9192736
DOI: 10.1093/oxfordjournals.jbchem.a021676
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15571
Akon Higuchi, Takashi Yoshimura, Kenichiro Kato, Mariko Hara, Masahiro Higuchi, Norihiko Minoura, Tomoko Hirasaki, Tetsuo Sato, Gen Ishikawa, Hiroo Nakano, Sakae Satoh, Sei-ichi Manabe
Sieving study of chromatin and histone-DNA complex by porous hollow fiber membranes
Journal of Membrane Science
1997
126:7-17
DOI: 10.1016/s0376-7388(96)00270-0
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1953
Ohno T, Itagaki H, Tanaka N, Ono H.
Validation study on five different cytotoxicity assays in Japan-an intermediate report
Toxicol In Vitro
1995
9(4):571-6
PubMed ID: 20650130
DOI: 10.1016/0887-2333(95)00041-6
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