Cell No. : Cell Name
RCB0271 : HeLa S3 (SC)
update : 2021/01/08
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| Comment | Suspension culturable. Useful for JIS medium inspection |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
|
Depositor |
Umeda, Makoto
|
| Originator |
Gey, G. O.
|
| Year of deposit |
1988
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
cervix
|
| Case history |
cervical carcinoma
|
| Classification |
cancer
|
| History |
JCRB
|
|
Lifespan |
infinite
|
| Morphology |
epithelial-like
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
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Adherent/Suspension cells
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| Medium and additives |
|
MEM + 10% CS
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| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% trypsin
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Culture information
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Passage ratio |
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1 : 8 split
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| SC frequency |
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Subculture : 1-2 times/week, Medium Renewal : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma |
|
(-)
|
| STR(human) |
|
OK
|
| Isozyme |
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LD, NP
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| Chromosome mode |
|
59-72(50) : /65(4),66(12),67(5),68(7),69(7),70(4)/
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
7
|
| User's Publication |
8
|
| Reference |
|
207
Prescott,D.M. (ed.)
Methods Cell Biol
1973
4:1-42
|
|
350
Kuroki T.
Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.
Exp Cell Res
1973
80(1):55-62
PubMed ID: 4361348
|
|
154
Hanaoka F, Yamada M
Localization of the replication point of mammalian cell DNA at the membrane.
Biochem Biophys Res Commun
1971
42:647-53
PubMed ID: 5543947
|
|
151
GOLDSTEIN, M N, SLOTNICK, I J, JOURNEY, L J
In vitro studies with HeLa cell line sensitive and resistant to actinomycin D.
Ann N Y Acad Sci
1960
89:474-83
PubMed ID: 13706673
|
|
1445
SATO G, FISHER HW, PUCK TT.
Molecular growth requirements of single mammalian cells.
Science
1957
126(3280):461-4
PubMed ID: 13486039
|
|
206
PUCK TT, MARCUS PI, CIECIURA SJ.
Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.
J Exp Med
1956
103(2):273-83
PubMed ID: 13286432
|
|
150
Gey,G.O., Coffman,W.D. and Kubicek,M.T.
Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.
Cancer Res
1952
12:264-265
|
| User's Publication |
|
6924
Yu Nakagawa et al
Relationship Among Structure, Cytotoxicity, and Michael Acceptor Reactivity of Quinocidin
Bioorganic & Medicinal Chemistry
2020
28(4):115308
PubMed ID: 31956051
|
|
9512
Sun Y, Tomura T, Sato J, Iizuka T, Fudou R, Ojika M.
Isolation and Biosynthetic Analysis of Haliamide, a New PKS-NRPS Hybrid Metabolite from the Marine Myxobacterium Haliangium ochraceum.
Molecules
2016
21:59
PubMed ID: 26751435
|
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6410
Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.
Is parainfluenza virus a threatening virus for human cancer cell lines?
Hum Cell
2009
22(3):81-4
PubMed ID: 19624309
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3165
Takamatsu, Shinji, Furukawa, Takako, Mori, Tetsuya, Yonekura, Yoshiharu, Fujibayashi, Yasuhisa
Noninvasive imaging of transplanted living functional cells transfected with a reporter estrogen receptor gene.
Nucl Med Biol
2005
32:821-9
PubMed ID: 16253806
|
|
3164
Takamatsu, Shinji, Inoue, Noboru, Katsumata, Toshiyuki, Nakamura, Katsumi, Fujibayashi, Yasuhisa, Takeuchi, Makoto
The relationship between the branch-forming glycosyltransferases and cell surface sugar chain structures.
Biochemistry
2005
44:6343-9
PubMed ID: 15835923
|
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6281
Okuda T, Sugiyama A, Niidome T, Aoyagi H.
Characters of dendritic poly(L-lysine) analogues with the terminal lysines replaced with arginines and histidines as gene carriers in vitro.
Biomaterials
2004
25(3):537-44
PubMed ID: 14585703
|
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3788
Ohsaki M, Okuda T, Wada A, Hirayama T, Niidome T, Aoyagi H.
In vitro gene transfection using dendritic poly(L-lysine).
Bioconjug Chem
2002
13(3):510-7
PubMed ID: 12009940
|
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6117
Niidome T, Aoyagi H.
Cationic α-Helical Peptides for Gene Delivery into Cells.
Methods Mol Med
2001
65:11-21
PubMed ID: 21318742
|
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