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Cell No. : Cell Name
RCB0271 : HeLa S3 (SC)  update : 2021/01/08
CommentSuspension culturable. Useful for JIS medium inspection
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Umeda, Makoto
Originator Gey, G. O.
Year of deposit 1988
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Case history cervical carcinoma
Classification cancer
History JCRB
Lifespan infinite
Morphology epithelial-like
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Medium and additives MEM + 10% CS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Isozyme LD, NP
Chromosome mode 59-72(50) : /65(4),66(12),67(5),68(7),69(7),70(4)/
deposit info
lot info
Reference information Reference 7
User's Publication 8

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207  Prescott,D.M. (ed.)    Methods Cell Biol  1973  4:1-42 
350  Kuroki T.  Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.  Exp Cell Res  1973  80(1):55-62  PubMed ID: 4361348
154  Hanaoka F, Yamada M  Localization of the replication point of mammalian cell DNA at the membrane.  Biochem Biophys Res Commun  1971  42:647-53  PubMed ID: 5543947
151  GOLDSTEIN, M N, SLOTNICK, I J, JOURNEY, L J  In vitro studies with HeLa cell line sensitive and resistant to actinomycin D.  Ann N Y Acad Sci  1960  89:474-83  PubMed ID: 13706673
1445  SATO G, FISHER HW, PUCK TT.  Molecular growth requirements of single mammalian cells.  Science  1957  126(3280):461-4  PubMed ID: 13486039
206  PUCK TT, MARCUS PI, CIECIURA SJ.  Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.  J Exp Med  1956  103(2):273-83  PubMed ID: 13286432
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265 

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User's Publication
6924  Yu Nakagawa et al  Relationship Among Structure, Cytotoxicity, and Michael Acceptor Reactivity of Quinocidin  Bioorganic & Medicinal Chemistry   2020  28(4):115308  PubMed ID: 31956051
9512  Sun Y, Tomura T, Sato J, Iizuka T, Fudou R, Ojika M.  Isolation and Biosynthetic Analysis of Haliamide, a New PKS-NRPS Hybrid Metabolite from the Marine Myxobacterium Haliangium ochraceum.  Molecules  2016  21:59  PubMed ID: 26751435
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309
3165  Takamatsu, Shinji, Furukawa, Takako, Mori, Tetsuya, Yonekura, Yoshiharu, Fujibayashi, Yasuhisa  Noninvasive imaging of transplanted living functional cells transfected with a reporter estrogen receptor gene.  Nucl Med Biol  2005  32:821-9  PubMed ID: 16253806
3164  Takamatsu, Shinji, Inoue, Noboru, Katsumata, Toshiyuki, Nakamura, Katsumi, Fujibayashi, Yasuhisa, Takeuchi, Makoto  The relationship between the branch-forming glycosyltransferases and cell surface sugar chain structures.  Biochemistry  2005  44:6343-9  PubMed ID: 15835923
6281  Okuda T, Sugiyama A, Niidome T, Aoyagi H.  Characters of dendritic poly(L-lysine) analogues with the terminal lysines replaced with arginines and histidines as gene carriers in vitro.  Biomaterials  2004  25(3):537-44  PubMed ID: 14585703
3788  Ohsaki M, Okuda T, Wada A, Hirayama T, Niidome T, Aoyagi H.  In vitro gene transfection using dendritic poly(L-lysine).  Bioconjug Chem  2002  13(3):510-7  PubMed ID: 12009940
6117  Niidome T, Aoyagi H.  Cationic α-Helical Peptides for Gene Delivery into Cells.  Methods Mol Med  2001  65:11-21  PubMed ID: 21318742

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