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Cell No. : Cell Name
RCB0271 : HeLa S3 (SC)  update : 2023/06/30
CommentSuspension culturable. Useful for JIS medium inspection
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Umeda, Makoto
Originator Gey, G. O.
Year of deposit 1988
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Disease name cervical carcinoma
Classification cancer
History JCRB
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_2934
deposit info
lot info
Medium Medium List
Culture type Adherent/Suspension cells
Culture medium MEM + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 59-72(50) : /65(4),66(12),67(5),68(7),69(7),70(4)/
STR(human) OK
deposit info
lot info
Reference information Reference 7
User's Publication 13

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207  Prescott,D.M. (ed.)    Methods Cell Biol  1973  4:1-42   
350  Kuroki T.  Colony formation of mammalian cells on agar plates and its application to Lederberg's replica plating.  Exp Cell Res  1973  80(1):55-62  PubMed ID: 4361348   DOI: 10.1016/0014-4827(73)90274-7
154  Hanaoka F, Yamada M  Localization of the replication point of mammalian cell DNA at the membrane.  Biochem Biophys Res Commun  1971  42:647-53  PubMed ID: 5543947   DOI: 10.1016/0006-291x(71)90537-7
151  GOLDSTEIN, M N, SLOTNICK, I J, JOURNEY, L J  In vitro studies with HeLa cell line sensitive and resistant to actinomycin D.  Ann N Y Acad Sci  1960  89:474-83  PubMed ID: 13706673   DOI: 10.1111/j.1749-6632.1960.tb20171.x
1445  SATO G, FISHER HW, PUCK TT.  Molecular growth requirements of single mammalian cells.  Science  1957  126(3280):461-4  PubMed ID: 13486039  
206  PUCK TT, MARCUS PI, CIECIURA SJ.  Clonal growth of mammalian cells in vitro; growth characteristics of colonies from single HeLa cells with and without a feeder layer.  J Exp Med  1956  103(2):273-83  PubMed ID: 13286432   DOI: 10.1084/jem.103.2.273
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265   

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User's Publication
16658  Sasaki K, Suzuki M, Sonoda T, Schneider-Poetsch T, Ito A, Takagi M, Fujishiro S, Sohtome Y, Dodo K, Umehara T, Aburatani H, Shin-Ya K, Nakao Y, Sodeoka M, Yoshida M.  Visualization of the dynamic interaction between nucleosomal histone H3K9 tri-methylation and HP1α chromodomain in living cells  Cell Chem Biol  2022  S2451-9456(22)00198-2  PubMed ID: 35728598   DOI: 10.1016/j.chembiol.2022.05.006
6924  Yu Nakagawa et al  Relationship Among Structure, Cytotoxicity, and Michael Acceptor Reactivity of Quinocidin  Bioorganic & Medicinal Chemistry   2020  28(4):115308  PubMed ID: 31956051   DOI: 10.1016/j.bmc.2020.115308
9512  Sun Y, Tomura T, Sato J, Iizuka T, Fudou R, Ojika M.  Isolation and Biosynthetic Analysis of Haliamide, a New PKS-NRPS Hybrid Metabolite from the Marine Myxobacterium Haliangium ochraceum.  Molecules  2016  21:59  PubMed ID: 26751435   DOI: 10.3390/molecules21010059
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x
3165  Takamatsu, Shinji, Furukawa, Takako, Mori, Tetsuya, Yonekura, Yoshiharu, Fujibayashi, Yasuhisa  Noninvasive imaging of transplanted living functional cells transfected with a reporter estrogen receptor gene.  Nucl Med Biol  2005  32:821-9  PubMed ID: 16253806   DOI: 10.1016/j.nucmedbio.2005.06.010
3164  Takamatsu, Shinji, Inoue, Noboru, Katsumata, Toshiyuki, Nakamura, Katsumi, Fujibayashi, Yasuhisa, Takeuchi, Makoto  The relationship between the branch-forming glycosyltransferases and cell surface sugar chain structures.  Biochemistry  2005  44:6343-9  PubMed ID: 15835923   DOI: 10.1021/bi047606a
6281  Okuda T, Sugiyama A, Niidome T, Aoyagi H.  Characters of dendritic poly(L-lysine) analogues with the terminal lysines replaced with arginines and histidines as gene carriers in vitro.  Biomaterials  2004  25(3):537-44  PubMed ID: 14585703   DOI: 10.1016/s0142-9612(03)00542-8
3788  Ohsaki M, Okuda T, Wada A, Hirayama T, Niidome T, Aoyagi H.  In vitro gene transfection using dendritic poly(L-lysine).  Bioconjug Chem  2002  13(3):510-7  PubMed ID: 12009940   DOI: 10.1021/bc015525a
6117  Niidome T, Aoyagi H.  Cationic α-Helical Peptides for Gene Delivery into Cells.  Methods Mol Med  2001  65:11-21  PubMed ID: 21318742   DOI: 10.1385/1-59259-139-6:11
2382  Niidome T, Takaji K, Urakawa M, Ohmori N, Wada A, Hirayama T, Aoyagi H  Chain length of cationic alpha-helical peptide sufficient for gene delivery into cells.  Bioconjug Chem  1999  10:773-80  PubMed ID: 10502342   DOI: 10.1021/bc990012d
18039  Nakagawa S, Okaya Y, Yatsunami K, Tanaka S, Ohtsu H, Fukui T, Watanabe T, Ichikawa A.  Identification of multiple regulatory elements of human L-histidine decarboxylase gene  J Biochem  1997  121(5):935-40  PubMed ID: 9192736   DOI: 10.1093/oxfordjournals.jbchem.a021676
15571  Akon Higuchi, Takashi Yoshimura, Kenichiro Kato, Mariko Hara, Masahiro Higuchi, Norihiko Minoura, Tomoko Hirasaki, Tetsuo Sato, Gen Ishikawa, Hiroo Nakano, Sakae Satoh, Sei-ichi Manabe  Sieving study of chromatin and histone-DNA complex by porous hollow fiber membranes  Journal of Membrane Science  1997  126:7-17    DOI: 10.1016/s0376-7388(96)00270-0
1953  Ohno T, Itagaki H, Tanaka N, Ono H.  Validation study on five different cytotoxicity assays in Japan-an intermediate report  Toxicol In Vitro  1995  9(4):571-6  PubMed ID: 20650130   DOI: 10.1016/0887-2333(95)00041-6

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