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Cell No. : Cell Name
RCB0270 : KG-1-C  update : 2022/09/22
CommentGlioma, S-100 Protein producing.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Miyake, Etsuo
Originator Miyake, Etsuo
Year of deposit 1988
Another name KG-1
Animal _human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 13 years
Tissue brain
Disease name brain tumor
Metastatic ability No
Classification cancer
Year of origin 1977
Lifespan infinite
Morphology other
Cellosaurus(Expasy) CVCL_2971
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/10-14 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 85-102(50) : /89(3),90(6),91(5),92(12),93(5),94(2)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 27


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Reference
182  Miyake E.  Establishment of a human single cell clonal line synthesizing nervous tissue specific protein (S-100 protein)  Fukuoka Igaku Zasshi  1985  76(10):500-7.  PubMed ID: 4093122  
738  Miyake E  Establishment of a human oligodendroglial cell line  Acta Neuropathol (Berl)  1979  46:51-55  PubMed ID: 222111   DOI: 10.1007/bf00684804

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User's Publication
19503  Cheng A, Kawahata I, Wang Y, Jia W, Wang H, Sekimori T, Chen Y, Suzuki H, Takeda A, Stefanova N, Finkelstein DI, Ma W, Chen M, Sasaki T, Fukunaga K.  Epsin2, a novel target for multiple system atrophy therapy via α-synuclein/FABP7 propagation  Brain  2023  146(8):3172-3180  PubMed ID: 37082980   DOI: 10.1093/brain/awad137
21369  Cheng A, Jia W, Finkelstein DI, Stefanova N, Wang H, Sasaki T, Kawahata I, Fukunaga K.  Pharmacological inhibition of FABP7 by MF 6 counteracts cerebellum dysfunction in an experimental multiple system atrophy mouse model.  Acta Pharmacol Sin  2023    PubMed ID: 37605049   DOI: 10.1038/s41401-023-01138-y
16852  Cheng A, Wang YF, Shinoda Y, Kawahata I, Yamamoto T, Jia WB, Yamamoto H, Mizobata T, Kawata Y, Fukunaga K.  Fatty acid-binding protein 7 triggers α-synuclein oligomerization in glial cells and oligodendrocytes associated with oxidative stress  Acta Pharmacol Sin  2022  43(3):552-562  PubMed ID: 33935286   DOI: 10.1038/s41401-021-00675-8
20075  Hisahara S, Iwahara N, Matsushita T, Suzuki S, Matsumura A, Fujikura M, Yokokawa K, Saito T, Manabe T, Kawamata J, Horio Y, Shimohama S.  SIRT1 decelerates morphological processing of oligodendrocyte cell lines and regulates the expression of cytoskeleton-related oligodendrocyte proteins.  Biochem Biophys Res Commun  2021  546:7-14  PubMed ID: 33556638   DOI: 10.1016/j.bbrc.2021.01.095
16375  Cheng A, Jia W, Kawahata I, Fukunaga K.  A novel fatty acid-binding protein 5 and 7 inhibitor ameliorates oligodendrocyte injury in multiple sclerosis mouse models  EBioMedicine  2021  ;72:103582  PubMed ID: 34624687   DOI: 10.1016/j.ebiom.2021.103582
13391  Cheng A, Kawahata I, Fukunaga K.  Fatty Acid Binding Protein 5 Mediates Cell Death by Psychosine Exposure through Mitochondrial Macropores Formation in Oligodendrocytes.  Biomedicines  2020    PubMed ID: 33419250   DOI: 10.3390/biomedicines8120635
4947  Matsuda Y, Ishiwata T, Yoshimura H, Hagio M, Arai T.  Inhibition of nestin suppresses stem cell phenotype of glioblastomas through the alteration of post-translational modification of heat shock protein HSPA8/HSC71  Cancer Lett  2015  357(2):602-1  PubMed ID: 25527454   DOI: 10.1016/j.canlet.2014.12.030
16012  Ohashi R, Matsuda Y, Ishiwata T, Naito Z.  Downregulation of fibroblast growth factor receptor 2 and its isoforms correlates with a high proliferation rate and poor prognosis in high-grade glioma  Oncol Rep  2014  32(3):1163-9  PubMed ID: 24968791   DOI: 10.3892/or.2014.3283
9480  Masatoshi Konno, Takafumi Hasegawa, Toru Baba, Emiko Miura, Naoto Sugeno, Akio Kikuchi, Fabienne C Fiesel, Tsutomu Sasaki, Masashi Aoki, Yasuto Itoyama, Atsushi Takeda  Suppression of Dynamin GTPase Decreases α-Synuclein Uptake by Neuronal and Oligodendroglial Cells: A Potent Therapeutic Target for Synucleinopathy  Mol Neurodegener  2012  7:38  PubMed ID: 22892036   DOI: 10.1186/1750-1326-7-38
7934  Matsuda Y, Kawamoto Y, Teduka K, Peng WX, Yamamoto T, Ishiwata T, Naito Z.  Morphological and cytoskeletal alterations of nervous system tumor cells with different culturing methods.  Int. J. Oncol.  2011  38:1253-8  PubMed ID: 21331444   DOI: 10.3892/ijo.2011.945
3400  Uchinokura, Shunro, Miyata, Shiro, Fukushima, Tsuyoshi, Itoh, Hiroshi, Nakano, Shinichi, Wakisaka, Shinichiro, Kataoka, Hiroaki  Role of hepatocyte growth factor activator (HGF activator) in invasive growth of human glioblastoma cells in vivo.  Int J Cancer  2006  118:583-92  PubMed ID: 16106403   DOI: 10.1002/ijc.21362
18825  Zhang Y, Iwasaki H, Wang H, Kudo T, Kalka TB, Hennet T, Kubota T, Cheng L, Inaba N, Gotoh M, Togayachi A, Guo J, Hisatomi H, Nakajima K, Nishihara S, Nakamura M, Marth JD, Narimatsu H.  Cloning and characterization of a new human UDP-N-acetyl-alpha-D-galactosamine:polypeptide N-acetylgalactosaminyltransferase, designated pp-GalNAc-T13, that is specifically expressed in neurons and synthesizes GalNAc alpha-serine/threonine antigen  J Biol Chem  2003  278(1):573-84  PubMed ID: 12407114   DOI: 10.1074/jbc.M203094200
19275  Matsunaga Y, Saito N, Fujii A, Yokotani J, Takakura T, Nishimura T, Esaki H, Yamada T.  A pH-dependent conformational transition of Abeta peptide and physicochemical properties of the conformers in the glial cell  Biochem J  2002  61(Pt 3):547-56.  PubMed ID: 11802784   DOI: 10.1042/0264-6021:3610547
6244  Harada H, Yoshida S, Nobe Y, Ezura Y, Atake T, Koguchi T, Emi M.  Genomic structure of the human NLK (nemo-like kinase) gene and analysis of its promoter region.  Gene  2002  285(1-2):175-82  PubMed ID: 12039044   DOI: 10.1016/s0378-1119(02)00412-2
13875  Satoh JI, Kuroda Y.  Alpha-synuclein expression is up-regulated in NTera2 cells during neuronal differentiation but unaffected by exposure to cytokines and neurotrophic factors  Parkinsonism Relat Disord  2001  8(1):7-17  PubMed ID: 11472875   DOI: 10.1016/s1353-8020(00)00075-4
16388  S. Yoshida, K. Fukino, H. Harada, H. Nagai, I. Imoto, J. Inazawa, H. Takahashi, A. Teramoto, M. Emi  The c-Jun NH2-terminal kinase3 (JNK3) gene: genomic structure, chromosomal assignment, and loss of expression in brain tumors  Journal of Human Genetics  2001  42:182-187  PubMed ID: 11322657   DOI: 10.1007/s100380170086
16913  Hamasuna R, Kataoka H, Meng JY, Itoh H, Moriyama T, Wakisaka S, Koono M.  Reduced expression of hepatocyte growth factor activator inhibitor type-2/placental bikunin (HAI-2/PB) in human glioblastomas: implication for anti-invasive role of HAI-2/PB in glioblastoma cells  Int J Cancer  2001  93(3):339-45  PubMed ID: 11433397   DOI: 10.1002/ijc.1349
5968  Satoh J, Kuroda Y.  Beta-catenin expression in human neural cell lines following exposure to cytokines and growth factors.  Neuropathology  2000  20(2):113-23  PubMed ID: 10935448   DOI: 10.1046/j.1440-1789.2000.00293.x
6258  Kurihara H, Zama A, Tamura M, Takeda J, Sasaki T, Takeuchi T.  Glioma/glioblastoma-specific adenoviral gene expression using the nestin gene regulator.  Gene Ther  2000  7(8):686-93  PubMed ID: 10800092   DOI: 10.1038/sj.gt.3301129
6263  Brown RC, Cascio C, Papadopoulos V.  Pathways of neurosteroid biosynthesis in cell lines from human brain: regulation of dehydroepiandrosterone formation by oxidative stress and beta-amyloid peptide.  J Neurochem  2000  74(2):847-59  PubMed ID: 10646538   DOI: 10.1046/j.1471-4159.2000.740847.x
18776  Satoh J, Kurohara K, Yukitake M, Kuroda Y.  Constitutive and cytokine-inducible expression of prion protein gene in human neural cell lines  J Neuropathol Exp Neurol  1998  57(2):131-9  PubMed ID: 9600205   DOI: 10.1097/00005072-199802000-00004
13873  Satoh J, Kurohara K, Yukitake M, Kuroda Y.  Interleukin-15, a T-cell growth factor, is expressed in human neural cell lines and tissues  J Neurol Sci  1998  155(2):170-7  PubMed ID: 9562262   DOI: 10.1016/s0022-510x(97)00310-9
892  Moriyama T, Kataoka H, Hamasuna R, Yokogami K, Uehara H, Kawano H, Goya T, Tsubouchi H, Koono M, Wakisaka S.  Up-regulation of vascular endothelial growth factor induced by hepatocyte growth factor/scatter factor stimulation in human glioma cells  Biochem Biophys Res Commun  1998  249(1):73-7  PubMed ID: 9705834   DOI: 10.1006/bbrc.1998.9078
18028  Iida M, Sunaga S, Hirota N, Kuribayashi N, Sakagami H, Takeda M, Matsumoto K.  Effect of glutathione-modulating compounds on hydrogen-peroxide-induced cytotoxicity in human glioblastoma and glioma cell lines  J Cancer Res Clin Oncol  1997  123(11-12):619-22  PubMed ID: 9620220   DOI: 10.1007/s004320050115
2059  Moriyama T, Kataoka H, Seguchi K, Tsubouchi H, Koono M  Effects of hepatocyte growth factor (HGF) on human glioma cells in vitro: HGF acts as a motility factor in glioma cells.  Int J Cancer  1996  66:678-85  PubMed ID: 8647632   DOI: 10.1002/(SICI)1097-0215(19960529)66:5<678::AID-IJC16>3.0.CO;2-0
17217  Tsurushima H, Liu SQ, Tsuboi K, Yoshii Y, Nose T, Ohno T.  Induction of human autologous cytotoxic T lymphocytes against minced tissues of glioblastoma multiforme  J Neurosurg  1996  84(2):258-63  PubMed ID: 8592229   DOI: 10.3171/jns.1996.84.2.0258
2058  Moriyama T, Kataoka H, Tsubouchi H, Koono M  Concomitant expression of hepatocyte growth factor (HGF), HGF activator and c-met genes in human glioma cells in vitro.  FEBS Lett  1995  372:78-82  PubMed ID: 7556648   DOI: 10.1016/0014-5793(95)00949-a


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