Cell No. : Cell Name
RCB0270 : KG-1-C
update : 2021/02/26
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| Comment | Glioma, S-100 Protein producing. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
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Basic information
|
Depositor |
Miyake, Etsuo
|
| Originator |
Miyake, Etsuo
|
| Year of deposit |
1988
|
| Another name |
KG-1
|
| Animal |
human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Race |
Japanese
|
| Gender |
Male
|
| Age at sampling |
13 years
|
| Tissue |
brain
|
| Disease name |
brain tumor
|
| Metastastatic ability |
No
|
| Classification |
cancer
|
| Year of origin |
1977
|
|
Lifespan |
infinite
|
| Morphology |
other
|
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| |
deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
|
| Medium and additives |
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DMEM (low glucose) + 10% FBS
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| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
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Subculture : once/10-14 days, Medium Renewal : 2 times/week
|
| Temperature |
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37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| STR(human) |
|
OK
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
85-102(50) : /89(3),90(6),91(5),92(12),93(5),94(2)/
|
| Images |
deposit info | lot info |
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| Reference information |
Reference |
1
|
| User's Publication |
9
|
| User's Publication |
|
13391
Cheng A, Kawahata I, Fukunaga K.
Fatty Acid Binding Protein 5 Mediates Cell Death by Psychosine Exposure through Mitochondrial Macropores Formation in Oligodendrocytes.
Biomedicines
2020
PubMed ID: 33419250
|
|
9480
Masatoshi Konno, Takafumi Hasegawa, Toru Baba, Emiko Miura, Naoto Sugeno, Akio Kikuchi, Fabienne C Fiesel, Tsutomu Sasaki, Masashi Aoki, Yasuto Itoyama, Atsushi Takeda
Suppression of Dynamin GTPase Decreases α-Synuclein Uptake by Neuronal and Oligodendroglial Cells: A Potent Therapeutic Target for Synucleinopathy
Mol Neurodegener
2012
7:38
PubMed ID: 22892036
|
|
7934
Matsuda Y, Kawamoto Y, Teduka K, Peng WX, Yamamoto T, Ishiwata T, Naito Z.
Morphological and cytoskeletal alterations of nervous system tumor cells with different culturing methods.
Int. J. Oncol.
2011
38:1253-8
PubMed ID: 21331444
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3400
Uchinokura, Shunro, Miyata, Shiro, Fukushima, Tsuyoshi, Itoh, Hiroshi, Nakano, Shinichi, Wakisaka, Shinichiro, Kataoka, Hiroaki
Role of hepatocyte growth factor activator (HGF activator) in invasive growth of human glioblastoma cells in vivo.
Int J Cancer
2006
118:583-92
PubMed ID: 16106403
|
|
6244
Harada H, Yoshida S, Nobe Y, Ezura Y, Atake T, Koguchi T, Emi M.
Genomic structure of the human NLK (nemo-like kinase) gene and analysis of its promoter region.
Gene
2002
285(1-2):175-82
PubMed ID: 12039044
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6258
Kurihara H, Zama A, Tamura M, Takeda J, Sasaki T, Takeuchi T.
Glioma/glioblastoma-specific adenoviral gene expression using the nestin gene regulator.
Gene Ther
2000
7(8):686-93
PubMed ID: 10800092
|
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6263
Brown RC, Cascio C, Papadopoulos V.
Pathways of neurosteroid biosynthesis in cell lines from human brain: regulation of dehydroepiandrosterone formation by oxidative stress and beta-amyloid peptide.
J Neurochem
2000
74(2):847-59
PubMed ID: 10646538
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2059
Moriyama T, Kataoka H, Seguchi K, Tsubouchi H, Koono M
Effects of hepatocyte growth factor (HGF) on human glioma cells in vitro: HGF acts as a motility factor in glioma cells.
Int J Cancer
1996
66:678-85
PubMed ID: 8647632
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2058
Moriyama T, Kataoka H, Tsubouchi H, Koono M
Concomitant expression of hepatocyte growth factor (HGF), HGF activator and c-met genes in human glioma cells in vitro.
FEBS Lett
1995
372:78-82
PubMed ID: 7556648
|
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