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Cell No. : Cell Name
RCB0270 : KG-1-C  update : 2021/02/26
CommentGlioma, S-100 Protein producing.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC( regarding any kind of for-profit use.
Basic information Depositor Miyake, Etsuo
Originator Miyake, Etsuo
Year of deposit 1988
Another name KG-1
Animal human < Mammals
Genus Homo
Species sapiens
Race Japanese
Gender Male
Age at sampling 13 years
Tissue brain
Disease name brain tumor
Metastastatic ability No
Classification cancer
Year of origin 1977
Lifespan infinite
Morphology other
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/10-14 days, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 85-102(50) : /89(3),90(6),91(5),92(12),93(5),94(2)/
deposit info
lot info
Reference information Reference 1
User's Publication 9

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738  Miyake E  Establishment of a human oligodendroglial cell line  Acta Neuropathol (Berl)  1979  46:51-55  PubMed ID: 222111

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User's Publication
13391  Cheng A, Kawahata I, Fukunaga K.  Fatty Acid Binding Protein 5 Mediates Cell Death by Psychosine Exposure through Mitochondrial Macropores Formation in Oligodendrocytes.  Biomedicines  2020    PubMed ID: 33419250
9480  Masatoshi Konno, Takafumi Hasegawa, Toru Baba, Emiko Miura, Naoto Sugeno, Akio Kikuchi, Fabienne C Fiesel, Tsutomu Sasaki, Masashi Aoki, Yasuto Itoyama, Atsushi Takeda  Suppression of Dynamin GTPase Decreases α-Synuclein Uptake by Neuronal and Oligodendroglial Cells: A Potent Therapeutic Target for Synucleinopathy  Mol Neurodegener  2012  7:38  PubMed ID: 22892036
7934  Matsuda Y, Kawamoto Y, Teduka K, Peng WX, Yamamoto T, Ishiwata T, Naito Z.  Morphological and cytoskeletal alterations of nervous system tumor cells with different culturing methods.  Int. J. Oncol.  2011  38:1253-8  PubMed ID: 21331444
3400  Uchinokura, Shunro, Miyata, Shiro, Fukushima, Tsuyoshi, Itoh, Hiroshi, Nakano, Shinichi, Wakisaka, Shinichiro, Kataoka, Hiroaki  Role of hepatocyte growth factor activator (HGF activator) in invasive growth of human glioblastoma cells in vivo.  Int J Cancer  2006  118:583-92  PubMed ID: 16106403
6244  Harada H, Yoshida S, Nobe Y, Ezura Y, Atake T, Koguchi T, Emi M.  Genomic structure of the human NLK (nemo-like kinase) gene and analysis of its promoter region.  Gene  2002  285(1-2):175-82  PubMed ID: 12039044
6258  Kurihara H, Zama A, Tamura M, Takeda J, Sasaki T, Takeuchi T.  Glioma/glioblastoma-specific adenoviral gene expression using the nestin gene regulator.  Gene Ther  2000  7(8):686-93  PubMed ID: 10800092
6263  Brown RC, Cascio C, Papadopoulos V.  Pathways of neurosteroid biosynthesis in cell lines from human brain: regulation of dehydroepiandrosterone formation by oxidative stress and beta-amyloid peptide.  J Neurochem  2000  74(2):847-59  PubMed ID: 10646538
2059  Moriyama T, Kataoka H, Seguchi K, Tsubouchi H, Koono M  Effects of hepatocyte growth factor (HGF) on human glioma cells in vitro: HGF acts as a motility factor in glioma cells.  Int J Cancer  1996  66:678-85  PubMed ID: 8647632
2058  Moriyama T, Kataoka H, Tsubouchi H, Koono M  Concomitant expression of hepatocyte growth factor (HGF), HGF activator and c-met genes in human glioma cells in vitro.  FEBS Lett  1995  372:78-82  PubMed ID: 7556648

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