Cell No. : Cell Name RCB0252 : W-V  update : 2024/02/13
Comment			SV40-transformed Werner's syndrome fibroblast
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Ohno, Tadao & Tanaka, Kiyoji
		Originator	Holliday, Robin
		Year of deposit	1988
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Tissue	skin
		Disease name	Werner's syndrome
		Classification	transformed
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_E079
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		DMEM (low glucose) + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : once/3-4 days
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Virus (HIV)		(-)
		Isozyme		LD, NP
		Chromosome mode		65-80(50) : /72(3),73(6),74(19),75(10),76(5),77(2)/
		STR(human)		OK
Reference information		Reference	3
		User's Publication	1

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Reference

1033  T Matsumura 1, F Malik, R Holliday  Levels of DNA methylation in diploid and SV40 transformed human fibroblasts  Exp Gerontol  1989  24(5-6):477-81  PubMed ID: 2561103   DOI: 10.1016/0531-5565(89)90054-5

1034  L I Huschtscha, K V Thompson, R Holliday  The susceptibility of Werner's syndrome and other human skin fibroblasts to SV40-induced transformation and immortalization.  Proc R Soc Lond B Biol Sci .  1986  229(1254):1-12  PubMed ID: 2878433   DOI: 10.1098/rspb.1986.0070

964  Thompson, K V, Holliday R  Genetic effects on the longevity of cultured human fibroblasts. I. Werner's syndrome.  Gerontology  1983  29:73-82  PubMed ID: 6840562   DOI: 10.1159/000213096

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User's Publication

5548  Kim JK, Ryll R, Ishizuka Y, Kato S.  Identification of cDNAs encoding two novel nuclear proteins, IMUP-1 and IMUP-2, upregulated in SV40-immortalized human fibroblasts.  Gene  2000  257(2):327-34  PubMed ID: 11080599   DOI: 10.1016/s0378-1119(00)00414-5