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Cell No. : Cell Name
RCB0252 : W-V  update : 2024/02/13
CommentSV40-transformed Werner's syndrome fibroblast
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao & Tanaka, Kiyoji
Originator Holliday, Robin
Year of deposit 1988
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Tissue skin
Disease name Werner's syndrome
Classification transformed
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_E079
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/3-4 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 65-80(50) : /72(3),73(6),74(19),75(10),76(5),77(2)/
STR(human) OK
Reference information Reference 3
User's Publication 2

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1033  T Matsumura 1, F Malik, R Holliday  Levels of DNA methylation in diploid and SV40 transformed human fibroblasts  Exp Gerontol  1989  24(5-6):477-81  PubMed ID: 2561103   DOI: 10.1016/0531-5565(89)90054-5
1034  L I Huschtscha, K V Thompson, R Holliday  The susceptibility of Werner's syndrome and other human skin fibroblasts to SV40-induced transformation and immortalization.  Proc R Soc Lond B Biol Sci .  1986  229(1254):1-12  PubMed ID: 2878433   DOI: 10.1098/rspb.1986.0070
964  Thompson, K V, Holliday R  Genetic effects on the longevity of cultured human fibroblasts. I. Werner's syndrome.  Gerontology  1983  29:73-82  PubMed ID: 6840562   DOI: 10.1159/000213096

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User's Publication
19027  Ishii G, Sangai T, Ito T, Hasebe T, Endoh Y, Sasaki H, Harigaya K, Ochiai A.  In vivo and in vitro characterization of human fibroblasts recruited selectively into human cancer stroma  Int J Cancer  2005  117(2):212-20  PubMed ID: 15900602   DOI: 10.1002/ijc.21199
5548  Kim JK, Ryll R, Ishizuka Y, Kato S.  Identification of cDNAs encoding two novel nuclear proteins, IMUP-1 and IMUP-2, upregulated in SV40-immortalized human fibroblasts.  Gene  2000  257(2):327-34  PubMed ID: 11080599   DOI: 10.1016/s0378-1119(00)00414-5

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