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Cell No. : Cell Name
RCB0251 : VA-13  update : 2024/11/12
CommentSV40-transformed WI-38.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Year of deposit 1988
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue lung
Classification transformed
Memo_1 Mycoplasma was eliminated with MC-110.
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_2759
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium HF-RITC80-7 (or MEM ALPHA) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma/Acholeplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 39-88(50) : /43(7),44(24),45(8),46(1),87(1),88(1)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 11


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Reference
1559  Ohno T, Kako R, Sato C, Ohkawa A  Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining.  Exp Cell Res  1986  163:309-16  PubMed ID: 3956580   DOI: 10.1016/0014-4827(86)90062-5
940  F Dolbeare, M Vanderlaan, W Phares  Alkaline phosphatase and an acid arylamidase as marker enzymes for normal and transformed WI-38 cells  J Histochem Cytochem  1980  28(5):419-26  PubMed ID: 6247391   DOI: 10.1177/28.5.6247391
1080  Girardi, A J, Weinstein D, Moorhead, P S  SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters.  Ann Med Exp Biol Fenn  1966  44:242-54  PubMed ID: 4290661  

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User's Publication
21766  Machitani M, Nomura A, Yamashita T, Yasukawa M, Ueki S, Fujita KI, Ueno T, Yamashita A, Tanzawa Y, Watanabe M, Taniguchi T, Saitoh N, Kaneko S, Kato Y, Mano H, Masutomi K.  Maintenance of R-loop structures by phosphorylated hTERT preserves genome integrity.  Nat Cell Biol  2024    PubMed ID: 38806647   DOI: 10.1038/s41556-024-01427-6
18688  Koike M, Ninomiya Y, Koike A.  Characterization of Ninjurin and TSC22 induction after X-irradiation of normal human skin cells  J Dermatol  2008  35(1):6-17  PubMed ID: 18181769   DOI: 10.1111/j.1346-8138.2007.00403.x
18885  Li N, Wu JL, Hasegawa T, Sakai J, Bai LM, Wang LY, Kakuta S, Furuya Y, Ogura H, Kataoka T, Tomida A, Tsuruo T, Ando M.  Bioactive polyketides from Peperomia duclouxii  J Nat Prod  2007  70(6):998-1001  PubMed ID: 17547458   DOI: 10.1021/np070089n
18622  Okumura H, Nakazawa J, Tsuganezawa K, Usui T, Osada H, Matsumoto T, Tanaka A, Yokoyama S.  Phenothiazine and carbazole-related compounds inhibit mitotic kinesin Eg5 and trigger apoptosis in transformed culture cells  Toxicol Lett  2006  166(1):44-52  PubMed ID: 16814965   DOI: 10.1016/j.toxlet.2006.05.011
18892  Wu JL, Li N, Hasegawa T, Sakai J, Mitsui T, Ogura H, Kataoka T, Oka S, Kiuchi M, Tomida A, Turuo T, Li M, Tang W, Ando M.  Bioactive secolignans from Peperomia dindygulensis  J Nat Prod  2006  69(5):790-4  PubMed ID: 16724842   DOI: 10.1021/np0600447
18882  Fu L, Zhang S, Li N, Wang J, Zhao M, Sakai J, Hasegawa T, Mitsui T, Kataoka T, Oka S, Kiuchi M, Hirose K, Ando M.  Three new triterpenes from Nerium oleander and biological activity of the isolated compounds  J Nat Prod  2005  68(2):198-206  PubMed ID: 15730243   DOI: 10.1021/np040072u
18888  Bioactive tetrahydrofuran lignans from Peperomia dindygulensis  Bioactive tetrahydrofuran lignans from Peperomia dindygulensis  J Nat Prod  2005  68(11):1656-60  PubMed ID: 16309318   DOI: 10.1021/np050283e
19027  Ishii G, Sangai T, Ito T, Hasebe T, Endoh Y, Sasaki H, Harigaya K, Ochiai A.  In vivo and in vitro characterization of human fibroblasts recruited selectively into human cancer stroma  Int J Cancer  2005  117(2):212-20  PubMed ID: 15900602   DOI: 10.1002/ijc.21199
17276  Tomatsu M, Kondo T, Yoshikawa T, Komeno T, Adachi N, Kawasaki Y, Ikuta A, Tashiro F.  An apoptotic inducer, aralin, is a novel type II ribosome-inactivating protein from Aralia elata  Biol Chem  2004  385(9):819-27  PubMed ID: 15493877   DOI: 10.1515/BC.2004.107
18648  Koishi R, Yoshimura C, Kohama T, Serizawa N.  Leustroducsin B activates nuclear factor-kappaB via the acidic sphingomyelinase pathway in human bone marrow-derived stromal cell line KM-102  J Interferon Cytokine Res  2002  22(3):343-50  PubMed ID: 12034042   DOI: 10.1089/107999002753675776
13730  Yatabe N, Kyo S, Kondo S, Kanaya T, Wang Z, Maida Y, Takakura M, Nakamura M, Tanaka M, Inoue M.  2-5A antisense therapy directed against human telomerase RNA inhibits telomerase activity and induces apoptosis without telomere impairment in cervical cancer cells  Cancer Gene Ther  2002  9(7):624-30  PubMed ID: 12082463   DOI: 10.1038/sj.cgt.7700479


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