Cell No. : Cell Name
RCB0251 : VA-13
update : 2023/06/30
|
| Comment | SV40-transformed WI-38. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Ohno, Tadao
|
| Year of deposit |
1988
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Tissue |
lung
|
| Classification |
transformed
|
| Memo_1 |
Mycoplasma was eliminated with MC-110.
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_2759
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
HF-RITC80-7 (or MEM ALPHA) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
39-88(50) : /43(7),44(24),45(8),46(1),87(1),88(1)
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
3
|
| User's Publication |
2
|
| Reference |
|
1559
Ohno T, Kako R, Sato C, Ohkawa A
Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining.
Exp Cell Res
1986
163:309-16
PubMed ID: 3956580
DOI: 10.1016/0014-4827(86)90062-5
|
|
940
F Dolbeare, M Vanderlaan, W Phares
Alkaline phosphatase and an acid arylamidase as marker enzymes for normal and transformed WI-38 cells
J Histochem Cytochem
1980
28(5):419-26
PubMed ID: 6247391
DOI: 10.1177/28.5.6247391
|
|
1080
Girardi, A J, Weinstein D, Moorhead, P S
SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters.
Ann Med Exp Biol Fenn
1966
44:242-54
PubMed ID: 4290661
|
| User's Publication |
|
17276
Tomatsu M, Kondo T, Yoshikawa T, Komeno T, Adachi N, Kawasaki Y, Ikuta A, Tashiro F.
An apoptotic inducer, aralin, is a novel type II ribosome-inactivating protein from Aralia elata
Biol Chem
2004
385(9):819-27
PubMed ID: 15493877
DOI: 10.1515/BC.2004.107
|
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13730
Yatabe N, Kyo S, Kondo S, Kanaya T, Wang Z, Maida Y, Takakura M, Nakamura M, Tanaka M, Inoue M.
2-5A antisense therapy directed against human telomerase RNA inhibits telomerase activity and induces apoptosis without telomere impairment in cervical cancer cells
Cancer Gene Ther
2002
9(7):624-30
PubMed ID: 12082463
DOI: 10.1038/sj.cgt.7700479
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