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Cell No. : Cell Name
RCB0251 : VA-13  update : 2022/03/09
CommentSV40-transformed WI-38.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Year of deposit 1988
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue lung
Classification transformed
Memo_1 Mycoplasma was eliminated with MC-110.
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_2759
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HF-RITC80-7 (or MEM ALPHA) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 39-88(50) : /43(7),44(24),45(8),46(1),87(1),88(1)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 3
User's Publication 1


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Reference
1559  Ohno T, Kako R, Sato C, Ohkawa A  Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining.  Exp Cell Res  1986  163:309-16  PubMed ID: 3956580  
940  F Dolbeare, M Vanderlaan, W Phares  Alkaline phosphatase and an acid arylamidase as marker enzymes for normal and transformed WI-38 cells  J Histochem Cytochem  1980  28(5):419-26  PubMed ID: 6247391   DOI: 10.1177/28.5.6247391
1080  Girardi, A J, Weinstein D, Moorhead, P S  SV40 transformation of human diploid cells. A parallel study of viral and karyologic parameters.  Ann Med Exp Biol Fenn  1966  44:242-54  PubMed ID: 4290661  

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User's Publication
13730  Yatabe N, Kyo S, Kondo S, Kanaya T, Wang Z, Maida Y, Takakura M, Nakamura M, Tanaka M, Inoue M.  2-5A antisense therapy directed against human telomerase RNA inhibits telomerase activity and induces apoptosis without telomere impairment in cervical cancer cells  Cancer Gene Ther  2002  9(7):624-30  PubMed ID: 12082463   DOI: 10.1038/sj.cgt.7700479



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