Cell No. : Cell Name
RCB0251 : VA-13
update : 2024/04/11
|
Comment | SV40-transformed WI-38. |
Comment from the depositor | |
Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
Remarks | |
Order Form |
Order Form(C-0005.pdf)
 
MTA(C-0007.pdf)
 
MTA(C-0007p.pdf)
 
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
|
|
Basic information
|
Depositor |
Ohno, Tadao
|
Year of deposit |
1988
|
Animal |
_human
< Mammals
|
Genus |
Homo
|
Species |
sapiens
|
Gender |
Female
|
Tissue |
lung
|
Classification |
transformed
|
Memo_1 |
Mycoplasma was eliminated with MC-110.
|
|
Lifespan |
infinite
|
Morphology |
fibroblast-like
|
Cellosaurus(Expasy) |
CVCL_2759
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
Culture medium |
|
HF-RITC80-7 (or MEM ALPHA) + 10% FBS
|
Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
SC frequency |
|
Subculture : 2 times/week
|
Temperature |
|
37
℃
|
CO2 concentration |
|
5
%
|
Freeze medium |
|
Medium + 10% DMSO
|
Freezing method |
|
Slow freezing
|
Mycoplasma |
|
(-)
|
Virus (HIV) |
|
(-)
|
Isozyme |
|
LD, NP
|
Chromosome mode |
|
39-88(50) : /43(7),44(24),45(8),46(1),87(1),88(1)
|
STR(human) |
|
OK
|
Images |
deposit info | lot info |
|
|
Reference information |
Reference |
3
|
User's Publication |
11
|
User's Publication |
21766
Machitani M, Nomura A, Yamashita T, Yasukawa M, Ueki S, Fujita KI, Ueno T, Yamashita A, Tanzawa Y, Watanabe M, Taniguchi T, Saitoh N, Kaneko S, Kato Y, Mano H, Masutomi K.
Maintenance of R-loop structures by phosphorylated hTERT preserves genome integrity.
Nat Cell Biol
2024
PubMed ID: 38806647
DOI: 10.1038/s41556-024-01427-6
|
18688
Koike M, Ninomiya Y, Koike A.
Characterization of Ninjurin and TSC22 induction after X-irradiation of normal human skin cells
J Dermatol
2008
35(1):6-17
PubMed ID: 18181769
DOI: 10.1111/j.1346-8138.2007.00403.x
|
18885
Li N, Wu JL, Hasegawa T, Sakai J, Bai LM, Wang LY, Kakuta S, Furuya Y, Ogura H, Kataoka T, Tomida A, Tsuruo T, Ando M.
Bioactive polyketides from Peperomia duclouxii
J Nat Prod
2007
70(6):998-1001
PubMed ID: 17547458
DOI: 10.1021/np070089n
|
18622
Okumura H, Nakazawa J, Tsuganezawa K, Usui T, Osada H, Matsumoto T, Tanaka A, Yokoyama S.
Phenothiazine and carbazole-related compounds inhibit mitotic kinesin Eg5 and trigger apoptosis in transformed culture cells
Toxicol Lett
2006
166(1):44-52
PubMed ID: 16814965
DOI: 10.1016/j.toxlet.2006.05.011
|
18892
Wu JL, Li N, Hasegawa T, Sakai J, Mitsui T, Ogura H, Kataoka T, Oka S, Kiuchi M, Tomida A, Turuo T, Li M, Tang W, Ando M.
Bioactive secolignans from Peperomia dindygulensis
J Nat Prod
2006
69(5):790-4
PubMed ID: 16724842
DOI: 10.1021/np0600447
|
18882
Fu L, Zhang S, Li N, Wang J, Zhao M, Sakai J, Hasegawa T, Mitsui T, Kataoka T, Oka S, Kiuchi M, Hirose K, Ando M.
Three new triterpenes from Nerium oleander and biological activity of the isolated compounds
J Nat Prod
2005
68(2):198-206
PubMed ID: 15730243
DOI: 10.1021/np040072u
|
18888
Bioactive tetrahydrofuran lignans from Peperomia dindygulensis
Bioactive tetrahydrofuran lignans from Peperomia dindygulensis
J Nat Prod
2005
68(11):1656-60
PubMed ID: 16309318
DOI: 10.1021/np050283e
|
19027
Ishii G, Sangai T, Ito T, Hasebe T, Endoh Y, Sasaki H, Harigaya K, Ochiai A.
In vivo and in vitro characterization of human fibroblasts recruited selectively into human cancer stroma
Int J Cancer
2005
117(2):212-20
PubMed ID: 15900602
DOI: 10.1002/ijc.21199
|
17276
Tomatsu M, Kondo T, Yoshikawa T, Komeno T, Adachi N, Kawasaki Y, Ikuta A, Tashiro F.
An apoptotic inducer, aralin, is a novel type II ribosome-inactivating protein from Aralia elata
Biol Chem
2004
385(9):819-27
PubMed ID: 15493877
DOI: 10.1515/BC.2004.107
|
18648
Koishi R, Yoshimura C, Kohama T, Serizawa N.
Leustroducsin B activates nuclear factor-kappaB via the acidic sphingomyelinase pathway in human bone marrow-derived stromal cell line KM-102
J Interferon Cytokine Res
2002
22(3):343-50
PubMed ID: 12034042
DOI: 10.1089/107999002753675776
|
13730
Yatabe N, Kyo S, Kondo S, Kanaya T, Wang Z, Maida Y, Takakura M, Nakamura M, Tanaka M, Inoue M.
2-5A antisense therapy directed against human telomerase RNA inhibits telomerase activity and induces apoptosis without telomere impairment in cervical cancer cells
Cancer Gene Ther
2002
9(7):624-30
PubMed ID: 12082463
DOI: 10.1038/sj.cgt.7700479
|