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Cell No. : Cell Name
RCB0249 : CKT-1  update : 2022/03/23
CommentVery flat and thin kidney epithelial cell
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Ohno, Tadao
Originator Kimura, Susumu
Year of deposit 1988
Animal bovine < Mammals
Genus Bos
Species taurus
Tissue kidney
Classification other
History IFO Takeuchi Masao
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_2403
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Chromosome mode 58-119(50) : 58(11),59(25),110(2),111(3),112(6)/
Reference information Reference 2
User's Publication 0

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637  Kimura S, Kamogashira T, Ota F, Fukui K, Yoshida N  Growth of bovine adenovirus type 3 in cells cloned from a cell line of calf kidney.  Tokushima J Exp Med  1971  18:33-7  PubMed ID: 5169311  
636  Kimura S, Fukui K, Yoshida N, Matsubara Y  A new cell line of calf kidney and its susceptibility to viruses.  Jpn J Microbiol  1968  12:293-8  PubMed ID: 4303468  

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