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Cell No. : Cell Name
RCB0248 : 3T6  update : 2022/03/08
CommentNon-contact inhibited
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Murakami, Yasufumi
Originator Todaro, J. & Green, H.
Year of deposit 1988
Animal mouse < Mammals
Age at sampling embryo
Tissue embryo
Classification transformed
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0601
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage cell No 3 x 10^(5) cells/60 mm dish
SC frequency Subculture : once/6 days, Medium Renewal : 2 times/6 days
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 67-75(50) : 67(5),68(7),69(7),70(10),71(10),72(2)/
Reference information Reference 1
User's Publication 0


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Reference
963  Todaro, G. and Green, H.  Quantitative studies of the growth of mouse embryo cells in culture and their development into established lines.  J Cell Biol  1963  17:299-313  PubMed ID: 13985244  

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