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細胞番号 : 細胞名
RCB0247 : 10T1/2  update : 2021/05/20
細胞特性(Comment:英)Fibroblast-like cells derived from mouse embryo. Can be used for experiments regarding cell transformation.
細胞特性(日)マウス胎児由来細胞。線維芽細胞様。形質転換実験に利用される。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず(cellbank.brc@riken.jp)までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Terasima, Toyozo
樹立者 Reznikoff, Catherine A.
寄託日 1988
別名 10T1/2(clone8)
動物種 _mouse < Mammals
動物系統名 C3H
embryo
採取組織 embryo
細胞分類 normal
細胞寿命 infinite
細胞形態 fibroblast-like
Cellosaurus(Expasy) CVCL_0190
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 BME (2mM L-Glutamine) + 10% FBS
抗生物質 Free
継代方法 0.25% trypsin
播種細胞数 5 x 10^(4) cells/60 mm dish
継代・培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
マウス系統検査 OK
アイソザイム検査 LD, NP
染色体検査 65-81(50) : /75(1),76(1),77(3),78(5),79(12),80(25)/
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 5件
Reference(日) 3件
利用者成果(英) 138件
利用者成果(日) 2件

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Reference(英)
2001  Weintraub H, Davis R, Tapscott S, Thayer M, Krause M, Benezra R, Blackwell, T K, Turner D, Rupp R, Hollenberg S, et al.  The myoD gene family: nodal point during specification of the muscle cell lineage.  Science  1991  251:761-6  PubMed ID: 1846704
962  Terasima T, Yasukawa M, Kimura M  Radiation-induced transformation of 10T 1/2 mouse cells in the plateau phase: post-irradiation changes and serum dependence.  Gann  1981  72:762-8  PubMed ID: 7327374
2000  Taylor, S M, Jones, P A  Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.  Cell  1979  17:771-9  PubMed ID: 90553
44  Paranjpe M, del Ande Eaton S, Boone, C W  Neoplasms produced from C3H/10T 1/2 cells attached to plastic plates; saturation density, anchorage dependence and serum requirement of in vitro lines correlated with growth aggressiveness in vivo.  J Cell Physiol  1978  96:63-71  PubMed ID: 659520
961  Reznikoff, C A, Brankow, D W, Heidelberger C  Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.  Cancer Res  1973  33:3231-8  PubMed ID: 4357355

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Reference(日)
1999  遠藤 剛  マウス:C3H10T1/2  生体の科学  1992  43:441 
1266  黒木登志夫  バイオテクノロジ-素材としての培養細胞 化学物質によるトランスホ-メ-ションとプロモーション  蛋白質 核酸 酵素  1989  34:257-268 
1238  達家雅明,角永武夫  バイオテクノロジ-素材としての培養細胞 増殖因子  蛋白質 核酸 酵素  1988  33:1230-1231 

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利用者成果(英)
20079  Miyoshi H, Abo K, Hosoya D, Matsuo K, Utsumi Y.  Effects of mouse fetal liver cell culture density on hematopoietic cell expansion in three-dimensional cocultures with stromal cells.  Int J Artif Organs  2021  391398821996377  PubMed ID: 33611956
14003  Yuzuriha A, Nakamura S, Sugimoto N, Kihara S, Nakagawa M, Yamamoto T, Sekiguchi K, Eto K.  Extracellular laminin regulates hematopoietic potential of pluripotent stem cells through integrin β1-ILK-β-catenin-JUN axis  Stem Cell Res  2021  53:102287.   PubMed ID: 33813173
20202  Matsunami A, Mizuno-Kamiya M, Kawaki H, Takayama E, Ueno K, Ando M, Morimoto-Ito H, Muramatsu Y, Sumitomo S, Kondoh N.  Augmented secretion of IL-1α from mouse oral squamous cell carcinoma (OSCC) cells caused by serum deprivation and hypoxia promotes immune suppressive activity of mesenchymal stromal cells.  J Oral Biosci  2021    PubMed ID: 34153475
13428  J.A.Lenis, P.Rico, J.L. GómezRibelles, M.A.Pacha-Olivenza, M.L.González-Martín, F.J.Bolívar  Structure, morphology, adhesion and in vitro biological evaluation of antibacterial multi-layer HA-Ag/SiO2/TiN/Ti coatings obtained by RF magnetron sputtering for biomedical applications  Materials Science and Engineering:  2020/1/1   
12022  Sampilvanjil A, Karasawa T, Yamada N, Komada T, Higashi T, Baatarjav C, Watanabe S, Kamata R, Ohno N, Takahashi M.  Cigarette smoke extract induces ferroptosis in vascular smooth muscle cells.  Am. J. Physiol. Heart Circ. Physiol.  2020    PubMed ID: 31975626
12066  van Gastel N, Stegen S, Eelen G, Schoors S, Carlier A, Daniëls VW, Baryawno N, Przybylski D, Depypere M, Stiers PJ, Lambrechts D, Van Looveren R, Torrekens S, Sharda A, Agostinis P, Lambrechts D, Maes F, Swinnen JV, Geris L, Van Oosterwyck H, Thienpont B, Carmeliet P, Scadden DT, Carmeliet G.  Lipid availability determines fate of skeletal progenitor cells via SOX9.  Nature  2020    PubMed ID: 32103177
13232  Sakai S, Yoshii A, Sakurai S, Horii K, Nagasuna O.  Silk fibroin nanofibers: a promising ink additive for extrusion three-dimensional bioprinting.  Mater Today Bio  2020    PubMed ID: 33083780
13376  Rico P, Rodrigo-Navarro A, Sánchez Pérez L, Salmeron-Sanchez M.  Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway.  Commun Biol  2020    PubMed ID: 33247189
13857  Gantumur E, Nakahata M, Kojima M, Sakai S.  Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation  Int J Bioprint  2020  6(1):250  PubMed ID: 32596552
14438  Kazuki Y, Uno N, Abe S, Kajitani N, Kazuki K, Yakura Y, Sawada C, Takata S, Sugawara M, Nagashima Y, Okada A, Hiratsuka M, Osaki M, Ferrari G, Tedesco FS, Nishikawa S, Fukumoto K, Takayanagi SI, Kunisato A, Kaneko S, Oshimura M, Tomizuka K.  Engineering of human induced pluripotent stem cells via human artificial chromosome vectors for cell therapy and disease modeling  Mol Ther Nucleic Acids  2020  23:629-639  PubMed ID: 33552683
11367  Ishijima Y, Ohmori S, Uneme A, Aoki Y, Kobori M, Ohida T, Arai M, Hosaka M, Ohneda K.  The Gata2 repression during 3T3-L1 preadipocyte differentiation is dependent on a rapid decrease in histone acetylation in response to glucocorticoid receptor activation.  Mol. Cell. Endocrinol.  2019    PubMed ID: 30615908
11397  Gantumur E, Sakai S, Nakahata M, Taya M.  Horseradish peroxidase-catalyzed hydrogelation consuming enzyme-produced hydrogen peroxide in the presence of reducing sugars.  Soft Matter  2019    PubMed ID: 30672948
11582  Numaga-Tomita T, Shimauchi T, Oda S, Tanaka T, Nishiyama K, Nishimura A, Birnbaumer L, Mori Y, Nishida M.  TRPC6 regulates phenotypic switching of vascular smooth muscle cells through plasma membrane potential-dependent coupling with PTEN.  FASEB J.  2019    PubMed ID: 31162976
11589  Aoki H, Yamashita M, Hashita T, Nakayama M, Yagi M, Iwao T, Matsunaga T.  Isolation of induced pluripotent stem cell-derived endothelial progenitor cells from sac-like structures.  Biochem. Biophys. Res. Commun.  2019  515(4):672-678  PubMed ID: 31178142
11681  Hiroe Morimoto-Ito, Masako Mizuno-Kamiya, Naoki Umemura, Yoshinori Inagaki, Eiji Takayama, Harumi Kawaki, Yasunori Muramatsu, Shinichiro Sumitomo, Nobuo Kondoh  Immunosuppressive Effect of Mesenchymal Stromal Cells is Enhanced by IL-1α from Oral Squamous Cell Carcinoma Cells  The Open Dentistry Journal  2019  13:221-227 
11738  Iriguchi S, Kaneko S.  In Vitro Differentiation of T Cells: From Nonhuman Primate-Induced Pluripotent Stem Cells.  Methods Mol. Biol.  2019    PubMed ID: 31396934
11743  Kawai Y, Kaneko S.  Redifferentiation of Adaptive Naïve-Like CTL from T-Cell-Derived iPSC.  Methods Mol. Biol.  2019    PubMed ID: 31396930
11765  Abe T, Sato T, Yoda T, Hoshi K.  The period circadian clock 2 gene responds to glucocorticoids and regulates osteogenic capacity.  Regen Ther  2019    PubMed ID: 31489343
11838  Niimi K, Adachi Y, Ishikawa H, Yamaguchi W, Kubota Y, Inagaki S, Furuyama T.  Endothelial specific deletion of FOXO1 alters pericyte coverage in the developing retina.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31601422
6647  Takahata Y, Nakamura E, Hata K, Wakabayashi M, Murakami T, Wakamori K, Yoshikawa H, Matsuda A, Fukui N, Nishimura R.  Sox4 is involved in osteoarthritic cartilage deterioration through induction of ADAMTS4 and ADAMTS5.  FASEB J  2019  33(1):619-630  PubMed ID: 30016600
6878  Yano F, Ohba S, Murahashi Y, Tanaka S, Saito T, Chung UI.  Runx1 contributes to articular cartilage maintenance by enhancement of cartilage matrix production and suppression of hypertrophic differentiation.  Sci Rep  2019  9(1):7666  PubMed ID: 31113964
6884  Iriguchi S, Kaneko S.  In Vitro Differentiation of T Cells: From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells.  Methods Mol Biol  2019  2048:59-70  PubMed ID: 31396929
6907  Tagaya H, Ishikawa K, Hosokawa Y, Kobayashi S, Ueoka Y, Shimada M, Ohashi Y, Mikami H, Yamamoto M, Ihara T, Kumazawa K, Sugihara K, Goshima N, Watanabe S, Semba K.  A method of producing genetically manipulated mouse mammary gland.  Breast Cancer Res  2019  21(1):1  PubMed ID: 30611295
9893  Zhongren Hu, Shurong Hong, Yu Zhang, Huijing Dai, Shuzhen Lin, Tingyu Yi, Hongmei Zhuang  Down-regulated WDR35 Contributes to Fetal Anomaly via Regulation of Osteogenic Differentiation  Gene  2019  20;697:48-56  PubMed ID: 30790652
12515  Baba TT, Miyazaki T, Ohara-Nemoto Y, Nemoto TK.  Suppressive effects of N-bisphosphonate in osteoblastic cells mitigated by non-N-bisphosphonate but not by sodium-dependent phosphate cotransporter inhibitor  Cell Biochem Funct  2019  37(6):400-407  PubMed ID: 31328801
14881  J.A. Lenis, G. Bejarano, P. Rico, J.L. Gómez Ribelles, F.J. Bolívar  Development of multilayer Hydroxyapatite - Ag/TiN-Ti coatings deposited by radio frequency magnetron sputtering with potential application in the biomedical field  Surface and Coatings Technology  2019  377:124856 
11014    Versatility of hydrogelation by dual-enzymatic reactions with oxidases and peroxidase  Biochemical Engineering Journal  2018  131, 1-8 
11047    Suppressive effect of mesenchymal stromal cells on interferon-γ-producing capability of spleen cells was specifically enhanced through humoral mediator(s) from mouse oral squamous cell carcinoma Sq-1979 Cells In Vitro  Cancer Translational Medicine  2018  4, 9-16 
11132  Ito Y, Nakamura S, Sugimoto N, Shigemori T, Kato Y, Ohno M, Sakuma S, Ito K, Kumon H, Hirose H, Okamoto H, Nogawa M, Iwasaki M, Kihara S, Fujio K, Matsumoto T, Higashi N, Hashimoto K, Sawaguchi A, Harimoto KI, Nakagawa M, Yamamoto T, Handa M, Watanabe N, Nishi E, Arai F, Nishimura S, Eto K.  Turbulence Activates Platelet Biogenesis to Enable Clinical Scale Ex Vivo Production.  Cell  2018  174(3):636-648  PubMed ID: 30017246
11182  Sakai S, Mochizuki K, Qu Y, Mail M, Nakahata M, Taya M.  Peroxidase-catalyzed microextrusion bioprinting of cell-laden hydrogel constructs in vaporized ppm-level hydrogen peroxide.  Biofabrication  2018  10(4):045007  PubMed ID: 30137024
11138  Fukuda M, Yoshizawa T, Karim MF, Sobuz SU, Korogi W, Kobayasi D, Okanishi H, Tasaki M, Ono K, Sawa T, Sato Y, Chirifu M, Masuda T, Nakamura T, Tanoue H, Nakashima K, Kobashigawa Y, Morioka H, Bober E, Ohtsuki S, Yamagata Y, Ando Y, Oike Y, Araki N, Takeda S, Mizuta H, Yamagata K.  SIRT7 has a critical role in bone formation by regulating lysine acylation of SP7/Osterix.  Nat Commun  2018  9(1):2833  PubMed ID: 30026585
4059  Yoshida K, Teramachi J, Uchibe K, Ikegame M, Qiu L, Yang D, Okamura H.  Reduction of protein phosphatase 2A Cα promotes in vivo bone formation and adipocyte differentiation.  Mol. Cell. Endocrinol.  2018  470:251-258  PubMed ID: 29128580
4109  Kiyama R, Nonoyama T, Wada S, Semba S, Kitamura N, Nakajima T, Kurokawa T, Yasuda K, Tanaka S, Gong JP.  Micro patterning of hydroxyapatite by soft lithography on hydrogels for selective osteoconduction.  Acta Biomater  2018    PubMed ID: 30292679
4110  Takei H, Edahiro Y, Mano S, Masubuchi N, Mizukami Y, Imai M, Morishita S, Misawa K, Ochiai T, Tsuneda S, Endo H, Nakamura S, Eto K, Ohsaka A, Araki M, Komatsu N.  Skewed megakaryopoiesis in human induced pluripotent stem cell-derived haematopoietic progenitor cells harbouring calreticulin mutations.  Br. J. Haematol.  2018    PubMed ID: 29741776
9466  Tetsuya Kawane 1, Xin Qin, Qing Jiang, Toshihiro Miyazaki, Hisato Komori, Carolina Andrea Yoshida, Viviane Keiko Dos Santos Matsuura-Kawata, Chiharu Sakane, Yuki Matsuo, Kazuhiro Nagai, Takafumi Maeno, Yuki Date, Riko Nishimura, Toshihisa Komori   Runx2 Is Required for the Proliferation of Osteoblast Progenitors and Induces Proliferation by Regulating Fgfr2 and Fgfr3  Sci Rep  2018  10;8(1):13551  PubMed ID: 30202094
14902  Kanayama S, Kaito T, Kitaguchi K, Ishiguro H, Hashimoto K, Chijimatsu R, Otsuru S, Takenaka S, Makino T, Sakai Y, Myoui A, Yoshikawa H.  ONO-1301 Enhances in vitro Osteoblast Differentiation and in vivo Bone Formation Induced by Bone Morphogenetic Protein  Spine (Phila Pa 1976)   2018  43(11):E616-E624  PubMed ID: 29016438
9773  Mellati A, Fan CM, Tamayol A, Annabi N, Dai S, Bi J, Jin B, Xian C, Khademhosseini A, Zhang H.  Microengineered 3D cell-laden thermoresponsive hydrogels for mimicking cell morphology and orientation in cartilage tissue engineering.  Biotechnol. Bioeng.  2017  114:217-231  PubMed ID: 27477393
10343  Khanmohammadi M, Sakai S, Taya M.  Fabrication of single and bundled filament-like tissues using biodegradable hyaluronic acid-based hollow hydrogel fibers.  Int. J. Biol. Macromol.  2017  104:204-212  PubMed ID: 28596006
11004    Migration and Osteogenic Differentiation of Mesenchymal Stem Cells on Titanium with a MicroPatterned Gelatin Matrix  International Journal of Theoretical and Applied Nanotechnology  2017  5:20-26 
6028  Kawamura F et al  Establishment of induced pluripotent stem cells from normal B cells and inducing AID expression in their differentiation into hematopoietic progenitor cells.  Sci Rep  2017  7(1):1659  PubMed ID: 28490810
6640  Haque Bhuyan MZ, Tamura Y, Sone E, Yoshinari Y, Maeda C, Takahashi M, Tabata Y, Murali R, Waki Y, Aoki K.  The intra-articular injection of RANKL-binding peptides inhibits cartilage degeneration in a murine model of osteoarthritis.  J Pharmacol Sci  2017  134(2):124-130  PubMed ID: 28648299
10050  Ha-Eun Yang, Yongjia Li, Akira Nishimura, Huei-Fen Jheng, Ana Yuliana, Ryuji Kitano-Ohue, Wataru Nomura, Nobuyuki Takahashi, Chu-Sook Kim, Rina Yu, Nahoko Kitamura, Si-Bum Park, Shigenobu Kishino, Jun Ogawa, Teruo Kawada, Tsuyoshi Goto  Synthesized Enone Fatty Acids Resembling Metabolites From Gut Microbiota Suppress Macrophage-Mediated Inflammation in Adipocytes  Mol Nutr Food Res  2017  Oct;61(10)  PubMed ID: 28544341
14049  Tamamura Y, Katsube K, Mera H, Itokazu M, Wakitani S.  Irx3 and Bmp2 regulate mouse mesenchymal cell chondrogenic differentiation in both a Sox9-dependent and -independent manner  J Cell Physiol  2017  232(12):3317-3336  PubMed ID: 28059449
14369  Mariko Kawai, Noriyuki Nagaoka, Yasuhiro Yoshida, Kiyoshi Ohura  Cell Population of Mesenchymal Stem Cells on Micro-patterned Titanium  Proceedings of the 2nd World Congress on Recent Advances in Nanotechnology  2017   
14639  Hirata S, Murata T, Suzuki D, Nakamura S, Jono-Ohnishi R, Hirose H, Sawaguchi A, Nishimura S, Sugimoto N, Eto K.  Selective Inhibition of ADAM17 Efficiently Mediates Glycoprotein Ibα Retention During Ex Vivo Generation of Human Induced Pluripotent Stem Cell-Derived Platelets  Stem Cells Transl Med  2017  6(3):720-730  PubMed ID: 28297575
14672  Shubhra QTH, Oyane A, Araki H, Nakamura M, Tsurushima H.  Calcium phosphate nanoparticles prepared from infusion fluids for stem cell transfection: process optimization and cytotoxicity analysis  Biomater Sci  2017  5(5):972-981  PubMed ID: 28211929
7113  Mellati A, Valizadeh Kiamahalleh M, Dai S, Bi J, Jin B, Zhang H.  Influence of polymer molecular weight on the in vitro cytotoxicity of poly (N-isopropylacrylamide).  Mater Sci Eng C Mater Biol Appl  2016  59:509-513  PubMed ID: 26652402
9730  Mellati A, Kiamahalleh MV, Madani SH, Dai S, Bi J, Jin B, Zhang H.  Poly(N-isopropylacrylamide) hydrogel/chitosan scaffold hybrid for three-dimensional stem cell culture and cartilage tissue engineering.  J Biomed Mater Res A  2016  104:2764-74  PubMed ID: 27325374
9786  Sakai S, Nomura K, Mochizuki K, Taya M.  Anchoring PEG-oleate to cell membranes stimulates reactive oxygen species production.  Colloids Surf B Biointerfaces  2016  147:336-342  PubMed ID: 27544656
9850  Park E, Kim MC, Choi CW, Kim J, Jin HS, Lee R, Lee JW, Park JH, Huh D, Jeong SY.  Effects of Dihydrophaseic Acid 3'-O-β-d-Glucopyranoside Isolated from Lycii radicis Cortex on Osteoblast Differentiation.  Molecules  2016  21(9). pii: E1260  PubMed ID: 27657033
9432  Patricia Rico Hayk Mnatsakanyan Matthew J. Dalby Manuel Salmerón‐Sánchez  Material‐Driven Fibronectin Assembly Promotes Maintenance of Mesenchymal Stem Cell Phenotypes  Adv Funct Mater  2016  26:6563–6573 
12975  Kaneko S.  In Vitro Generation of Antigen-Specific T Cells from Induced Pluripotent Stem Cells of Antigen-Specific T Cell Origin  Methods Mol Biol  2016  1393:67-73  PubMed ID: 27033216
13478  Saitsu H, Sonoda M, Higashijima T, Shirozu H, Masuda H, Tohyama J, Kato M, Nakashima M, Tsurusaki Y, Mizuguchi T, Miyatake S, Miyake N, Kameyama S, Matsumoto N.  Somatic mutations in GLI3 and OFD1 involved in sonic hedgehog signaling cause hypothalamic hamartoma  Ann Clin Transl Neurol  2016  3(5):356-65  PubMed ID: 27231705
14126  Moreau T, Evans AL, Vasquez L, Tijssen MR, Yan Y, Trotter MW, Howard D, Colzani M, Arumugam M, Wu WH, Dalby A, Lampela R, Bouet G, Hobbs CM, Pask DC, Payne H, Ponomaryov T, Brill A, Soranzo N, Ouwehand WH, Pedersen RA, Ghevaert C.  Large-scale production of megakaryocytes from human pluripotent stem cells by chemically defined forward programming  Nat Commun  2016  7:11208  PubMed ID: 27052461
7378  Sakai S, Liu Y, Sengoku M, Taya M.  Cell-selective encapsulation in hydrogel sheaths via biospecific identification and biochemical cross-linking.  Biomaterials  2015  53:494-501  PubMed ID: 25890746
9405  Yoshikazu Mikami, Kiyofumi Yamamoto, Yuko Akiyama, Masayuki Kobayashi, Eri Watanabe, Nobukazu Watanabe, Masatake Asano, Noriyoshi Shimizu, Kazuo Komiyama  Osteogenic Gene Transcription Is Regulated via Gap Junction-Mediated Cell-Cell Communication  Stem Cells Dev  2015  24(2):214-27  PubMed ID: 25137151
10322  Ryo Nishikiori, Kotaro Watanabe, Koichi Kato  Antibody Arrays for Quality Control of Mesenchymal Stem Cells  ACS Appl Mater Interfaces  2015  7(30):16828-36  PubMed ID: 26172315
15142  Suttinont C, Mashimo Y, Mie M, Kobatake E.  Delivery of bFGF for Tissue Engineering by Tethering to the ECM  Biomed Res Int  2015  2015:208089  PubMed ID: 26539469
3999  Hirata N et al  A chemical probe that labels human pluripotent stem cells.  Cell Rep  2014  6(6):1165-1174  PubMed ID: 24613351
14698  Nakahara F, Kitaura J, Uchida T, Nishida C, Togami K, Inoue D, Matsukawa T, Kagiyama Y, Enomoto Y, Kawabata KC, Chen-Yi L, Komeno Y, Izawa K, Oki T, Nagae G, Harada Y, Harada H, Otsu M, Aburatani H, Heissig B, Hattori K, Kitamura T.  Hes1 promotes blast crisis in chronic myelogenous leukemia through MMP-9 upregulation in leukemic cells  Blood  2014  123(25):3932-42  PubMed ID: 24825862
15321  Kitaura Y, Hojo H, Komiyama Y, Takato T, Chung UI, Ohba S.  Gli1 haploinsufficiency leads to decreased bone mass with an uncoupling of bone metabolism in adult mice  PLoS One  2014  9(10):e109597  PubMed ID: 25313900
6001  Ogasawara T, Ohba S, Yano F, Kawaguchi H, Chung UI, Saito T, Yonehara Y, Nakatsuka T, Mori Y, Takato T, Hoshi K.  Nanog promotes osteogenic differentiation of the mouse mesenchymal cell line C3H10T1/2 by modulating bone morphogenetic protein (BMP) signaling.  J Cell Physiol  2013  228(1):163-71  PubMed ID: 22585661
6073  Nakagawa Y, Nakamura S, Nakajima M, Endo H, Dohda T, Takayama N, Nakauchi H, Arai F, Fukuda T, Eto K.  Two differential flows in a bioreactor promoted platelet generation from human pluripotent stem cell-derived megakaryocytes.  Exp Hematol  2013  41(8):742-8  PubMed ID: 23618622
6578  Zanotti S1, Canalis E.  Notch suppresses nuclear factor of activated T cells (NFAT) transactivation and Nfatc1 expression in chondrocytes.  Endocrinology  2013  154(2):762-72  PubMed ID: 23264614
6572  Kan A, Ikeda T, Fukai A, Nakagawa T, Nakamura K, Chung UI, Kawaguchi H, Tabin CJ.  SOX11 contributes to the regulation of GDF5 in joint maintenance.  BMC Dev Biol  2013  13:4  PubMed ID: 23356643
6575  Hata K, Takashima R, Amano K, Ono K, Nakanishi M, Yoshida M, Wakabayashi M, Matsuda A, Maeda Y, Suzuki Y, Sugano S, Whitson RH, Nishimura R, Yoneda T.  Arid5b facilitates chondrogenesis by recruiting the histone demethylase Phf2 to Sox9-regulated genes.  Nat Commun  2013  4:2850  PubMed ID: 24276541
13742  Kishimoto KN, Itoi E.  Lithium chloride enhances cathepsin H expression and BMP-4 degradation in C3H10T1/2 cells  Biomed Res Int  2013  2013:143742  PubMed ID: 24312905
5991  Takayama N, Eto K.  In vitro generation of megakaryocytes and platelets from human embryonic stem cells and induced pluripotent stem cells.  Methods Mol Biol  2012  788:205-17  PubMed ID: 22130710
6012  Zhao WH1, Hu ZQ.  Up-regulation of IL-33 expression in various types of murine cells by IL-3 and IL-4.  Cytokine  2012  58(2):267-73  PubMed ID: 22370606
5318  Gu S, Boyer TG, Naski MC.  Basic helix-loop-helix transcription factor Twist1 inhibits transactivator function of master chondrogenic regulator Sox9.  J Biol Chem.   2012  287(25):21082-92  PubMed ID: 22532563
6554  Oze H, Hirao M, Ebina K, Shi K, Kawato Y, Kaneshiro S, Yoshikawa H, Hashimoto J.  Impact of medium volume and oxygen concentration in the incubator on pericellular oxygen concentration and differentiation of murine chondrogenic cell culture.  In Vitro Cell Dev Biol Anim  2012  48(2):123-30  PubMed ID: 22259015
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3183  Hata, Kenji, Nishimura, Riko, Ueda, Mio, Ikeda, Fumiyo, Matsubara, Takuma, Ichida, Fumitaka, Hisada, Kunihiro, Nokubi, Takashi, Yamaguchi, Akira, Yoneda, Toshiyuki  A CCAAT/enhancer binding protein beta isoform, liver-enriched inhibitory protein, regulates commitment of osteoblasts and adipocytes.  Mol Cell Biol  2005  25:1971-9  PubMed ID: 15713650
6876  Imai Y, Terai H, Nomura-Furuwatari C, Mizuno S, Matsumoto K, Nakamura T, Takaoka K.  Hepatocyte growth factor contributes to fracture repair by upregulating the expression of BMP receptors.  J Bone Miner Res  2005  20(10):1723-30  PubMed ID: 16160730
3158  Nishimura, Haruki, Nishimura, Masahiro, Oda, Ryo, Yamanaka, Katsuyuki, Matsubara, Takehiro, Ozaki, Yoshie, Sekiya, Kensuke, Hamada, Taizo, Kato, Yukio  Lectins induce resistance to proteases and/or mechanical stimulus in all examined cells--including bone marrow mesenchymal stem cells--on various scaffolds.  Exp Cell Res  2004  295:119-27  PubMed ID: 15051495
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2635  Hata K, Nishimura R, Ikeda F, Yamashita K, Matsubara T, Nokubi T, Yoneda T  Differential roles of Smad1 and p38 kinase in regulation of peroxisome proliferator-activating receptor gamma during bone morphogenetic protein 2-induced adipogenesis.  Mol Biol Cell  2003  14:545-55  PubMed ID: 12589053
4944  Oda R, Suardita K, Fujimoto K, Pan H, Yan W, Shimazu A, Shintani H, Kato Y.  Anti-membrane-bound transferrin-like protein antibodies induce cell-shape change and chondrocyte differentiation in the presence or absence of concanavalin A.  J Cell Sci  2003  116(Pt 10):2029-38  PubMed ID: 12679380
5039  Murata T, Sugatani T, Shimizu K, Manganiello VC, Tagawa T.  Effect of the phosphodiesterase 4 inhibitor, rolipram, on retinoic acid-increased alkaline phosphatase activity in the mouse fibroblastic C3H10T1/2 cell line.  Arch Oral Biol  2003  48(1):63-7  PubMed ID: 12615143
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3327  寛山隆、三原田賢一、青木尚子、須藤和寛、中村幸夫  10T1/2を用いた胚性幹細胞由来血液細胞の長期培養系の確立  第5回日本再生医療学会総会  2006   
3320  石垣太郎、寛山隆、三原田賢一、藤岡剛、須藤和寛、檀上稲穂、長澤俊郎、中村幸夫  臍帯血培養系における Feeder細胞(OP9,10T1/2)の比較  第28回日本分子生物学会  2005   



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