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細胞番号 : 細胞名
RCB0247 : 10T1/2  update : 2024/10/04
細胞特性(Comment:英)Fibroblast-like cells derived from mouse embryo. Can be used for experiments regarding cell transformation.
細胞特性(日)マウス胎児由来細胞。線維芽細胞様。形質転換実験に利用される。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Terasima, Toyozo
樹立者 Reznikoff, Catherine A.
寄託日 1988
別名 10T1/2(clone8)
動物種 _mouse < Mammals
動物系統名 C3H
embryo
採取組織 embryo
細胞分類 normal
細胞寿命 infinite
細胞形態 fibroblast-like
Cellosaurus(Expasy) CVCL_0190
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 BME (2mM L-Glutamine) + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
播種細胞数 5 x 10 4 cells/60 mm dish
継代・培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ/アコレプラズマ (-)
マウス系統検査 OK
アイソザイム検査 LD, NP
染色体検査 65-81(50) : /75(1),76(1),77(3),78(5),79(12),80(25)/
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 6件
Reference(日) 3件
利用者成果(英) 193件
利用者成果(日) 0件

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Reference(英)
2001  Weintraub H, Davis R, Tapscott S, Thayer M, Krause M, Benezra R, Blackwell, T K, Turner D, Rupp R, Hollenberg S, et al.  The myoD gene family: nodal point during specification of the muscle cell lineage.  Science  1991  251:761-6  PubMed ID: 1846704   DOI: 10.1126/science.1846704
962  Terasima T, Yasukawa M, Kimura M  Radiation-induced transformation of 10T 1/2 mouse cells in the plateau phase: post-irradiation changes and serum dependence.  Gann  1981  72:762-8  PubMed ID: 7327374  
2000  Taylor, S M, Jones, P A  Multiple new phenotypes induced in 10T1/2 and 3T3 cells treated with 5-azacytidine.  Cell  1979  17:771-9  PubMed ID: 90553   DOI: 10.1016/0092-8674(79)90317-9
44  Paranjpe M, del Ande Eaton S, Boone, C W  Neoplasms produced from C3H/10T 1/2 cells attached to plastic plates; saturation density, anchorage dependence and serum requirement of in vitro lines correlated with growth aggressiveness in vivo.  J Cell Physiol  1978  96:63-71  PubMed ID: 659520   DOI: 10.1002/jcp.1040960108
961  Reznikoff, C A, Brankow, D W, Heidelberger C  Establishment and characterization of a cloned line of C3H mouse embryo cells sensitive to postconfluence inhibition of division.  Cancer Res  1973  33:3231-8  PubMed ID: 4357355  
176  Reznikoff CA, Bertram JS, Brankow DW, Heidelberger C.  Quantitative and qualitative studies of chemical transformation of cloned C3H mouse embryo cells sensitive to postconfluence inhibition of cell division  Cancer Res  1973  33(12):3239-49  PubMed ID: 4796800  

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Reference(日)
1999  遠藤 剛  マウス:C3H10T1/2  生体の科学  1992  43:441   
1266  黒木登志夫  バイオテクノロジ-素材としての培養細胞 化学物質によるトランスホ-メ-ションとプロモーション  蛋白質 核酸 酵素  1989  34:257-268   
1238  達家雅明,角永武夫  バイオテクノロジ-素材としての培養細胞 増殖因子  蛋白質 核酸 酵素  1988  33:1230-1231   

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利用者成果(英)
21783  Takayanagi SI, Chuganji S, Tanaka M, Wang B, Hasegawa S, Fukumoto K, Wasano N, Kakitani M, Ochiai N, Kawai Y, Ueda T, Ishikawa A, Kurimoto Y, Fukui A, Kamibayashi S, Imai E, Kunisato A, Nozawa H, Kaneko S.  A culture method with berbamine, a plant alkaloid, enhances CAR-T cell efficacy through modulating cellular metabolism.  Commun Biol  2024  7(1):685  PubMed ID: 38834758   DOI: 10.1038/s42003-024-06297-0
21798  Ichiyama-Kobayashi S, Hata K, Wakamori K, Takahata Y, Murakami T, Yamanaka H, Takano H, Yao R, Uzawa N, Nishimura R.  Chromatin profiling identifies chondrocyte-specific Sox9 enhancers important for skeletal development.  JCI Insight  2024  9(11)  PubMed ID: 38855864   DOI: 10.1172/jci.insight.175486
21965  Hidaka M, Kojima M, Sakai S.  Micromixer driven by bubble-induced acoustic microstreaming for multi-ink 3D bioprinting.  Lab Chip  2024    PubMed ID: 39221588   DOI: 10.1039/d4lc00552j
22005  Nomura K, Kimira Y, Kobayashi R, Shiobara Y, Osawa Y, Kataoka-Matsushita A, Shimizu J, Wada M, Mano H.  Collagen-derived dipeptide prolyl-hydroxyproline cooperates with Foxg1 to activate the PGC-1α promoter and induce brown adipocyte-like phenotype in rosiglitazone-treated C3H10T1/2 cells  Front Nutr  2024  11:1375532  PubMed ID: 38812940   DOI: 10.3389/fnut.2024.1375532
22021  Goto R, Sakai S, Delattre C, Petit E, El Boutachfaiti R, Nakahata M.  Enzymatically cross-linkable sulfated bacterial polyglucuronic acid as an affinity-based carrier of FGF-2 for therapeutic angiogenesis.  J Biosci Bioeng  2024    PubMed ID: 39343697   DOI: 10.1016/j.jbiosc.2024.08.011
22163  Sato S, Hanai T, Kanamoto T, Kawano F, Hikida M, Yokoi H, Take Y, Magome T, Ebina K, Mae T, Tanaka H, Nakata K.  Vibration acceleration enhances proliferation, migration, and maturation of C2C12 cells and promotes regeneration of muscle injury in male rats.  Physiol Rep  2024  12(4):e15905  PubMed ID: 38396237   DOI: 10.14814/phy2.15905
17732  Higa Y, Hiasa M, Tenshin H, Nakaue E, Tanaka M, Kim S, Nakagawa M, Shimizu S, Tanimoto K, Teramachi J, Harada T, Oda A, Oura M, Sogabe K, Hara T, Sumitani R, Maruhashi T, Yamagami H, Endo I, Matsumoto T, Tanaka E, Abe M.  The Xanthine Oxidase Inhibitor Febuxostat Suppresses Adipogenesis and Activates Nrf2  Antioxidants (Basel)  2023  12(1):133  PubMed ID: 36670994   DOI: 10.3390/antiox12010133
19663  Kotani T, Mubarok W, Hananouchi T, Sakai S.  Horseradish Peroxidase-Mediated Bioprinting via Bioink Gelation by Alternately Extruded Support Material  ACS Biomater Sci Eng  2023  9(10):5804-5812  PubMed ID: 37738620   DOI: 10.1021/acsbiomaterials.3c00996
19690  Liu C, Imai M, Edahiro Y, Mano S, Takei H, Nudejima M, Kurose A, Morishita S, Ando M, Tsuneda S, Araki M, Komatsu N.  Establishment of isogenic induced pluripotent stem cells with or without pathogenic mutation for understanding the pathogenesis of myeloproliferative neoplasms  Exp Hematol  2023  118:12-20  PubMed ID: 36511286   DOI: 10.1016/j.exphem.2022.11.005
19966  Yu Y, Uchida-Fukuhara Y, Weng Y, He Y, Ikegame M, Wang Z, Yoshida K, Okamura H, Qiu L.  Neuropilin 1 (NRP1) Positively Regulates Adipogenic Differentiation in C3H10T1/2 Cells  Int J Mol Sci  2023  24(8):7394  PubMed ID: 37108554   DOI: 10.3390/ijms24087394
17601  Furuya Y, Mera H, Itokazu M, Terai S, Nakamura H, Wakitani S, Yasuda H.  Induction of chondrogenesis with a RANKL-binding peptide, WP9QY, in vitro and in vivo in a rabbit model  Biochem Biophys Res Commun  2022  602:98-104  PubMed ID: 35259590   DOI: 10.1016/j.bbrc.2022.03.019
20079  Miyoshi H, Abo K, Hosoya D, Matsuo K, Utsumi Y.  Effects of mouse fetal liver cell culture density on hematopoietic cell expansion in three-dimensional cocultures with stromal cells.  Int J Artif Organs  2022  391398821996377  PubMed ID: 33611956   DOI: 10.1177/0391398821996377
16273  Sakai S, Kotani T, Harada R, Goto R, Morita T, Bouissil S, Dubessay P, Pierre G, Michaud P, El Boutachfaiti R, Nakahata M, Kojima M, Petit E, Delattre C.  Development of phenol-grafted polyglucuronic acid and its application to extrusion-based bioprinting inks  Carbohydr Polym  2022  277:118820  PubMed ID: 34893237   DOI: 10.1016/j.carbpol.2021.118820
20692  Hayashi Y, Kawabata KC, Tanaka Y, Uehara Y, Mabuchi Y, Murakami K, Nishiyama A, Kiryu S, Yoshioka Y, Ota Y, Sugiyama T, Mikami K, Tamura M, Fukushima T, Asada S, Takeda R, Kunisaki Y, Fukuyama T, Yokoyama K, Uchida T, Hagihara M, Ohno N, Usuki K, Tojo A, Katayama Y, Goyama S, Arai F, Tamura T, Nagasawa T, Ochiya T, Inoue D, Kitamura T.  MDS cells impair osteolineage differentiation of MSCs via extracellular vesicles to suppress normal hematopoiesis.  Cell Rep  2022  39(6):110805  PubMed ID: 35545056   DOI: 10.1016/j.celrep.2022.110805
16754  Ishihara K, Kaneyasu M, Fukazawa K, Zhang R, Teramura Y.  Induction of mesenchymal stem cell differentiation by co-culturing with mature cells in double-layered 2-methacryloyloxyethyl phosphorylcholine polymer hydrogel matrices  J Mater Chem B  2022  10(14):2561-2569  PubMed ID: 34878485   DOI: 10.1039/d1tb01817e
16755  Shimohata N, Harada Y, Hayano T.  Proteomic analysis of nascent polypeptide chains that potentially induce translational pausing during elongation  Biosci Biotechnol Biochem  2022  zbac097  PubMed ID: 35749475   DOI: 10.1093/bbb/zbac097
21046  Goto R, Nakahata M, Sakai S.  Phenol-Grafted Alginate Sulfate Hydrogel as an Injectable FGF-2 Carrier.  Gels  2022  8(12)  PubMed ID: 36547342   DOI: 10.3390/gels8120818
14003  Yuzuriha A, Nakamura S, Sugimoto N, Kihara S, Nakagawa M, Yamamoto T, Sekiguchi K, Eto K.  Extracellular laminin regulates hematopoietic potential of pluripotent stem cells through integrin β1-ILK-β-catenin-JUN axis  Stem Cell Res  2021  53:102287.   PubMed ID: 33813173   DOI: 10.1016/j.scr.2021.102287
20202  Matsunami A, Mizuno-Kamiya M, Kawaki H, Takayama E, Ueno K, Ando M, Morimoto-Ito H, Muramatsu Y, Sumitomo S, Kondoh N.  Augmented secretion of IL-1α from mouse oral squamous cell carcinoma (OSCC) cells caused by serum deprivation and hypoxia promotes immune suppressive activity of mesenchymal stromal cells.  J Oral Biosci  2021    PubMed ID: 34153475   DOI: 10.1016/j.job.2021.06.001
16752  Ishii M, Ando J, Yamazaki S, Toyota T, Ohara K, Furukawa Y, Suehara Y, Nakanishi M, Nakashima K, Ohshima K, Nakauchi H, Ando M.  iPSC-Derived Neoantigen-Specific CTL Therapy for Ewing Sarcoma  Cancer Immunol Res  2021  9(10):1175-1186  PubMed ID: 34385178   DOI: 10.1158/2326-6066.CIR-21-0193
12022  Sampilvanjil A, Karasawa T, Yamada N, Komada T, Higashi T, Baatarjav C, Watanabe S, Kamata R, Ohno N, Takahashi M.  Cigarette smoke extract induces ferroptosis in vascular smooth muscle cells.  Am. J. Physiol. Heart Circ. Physiol.  2020    PubMed ID: 31975626   DOI: 10.1152/ajpheart.00559.2019
12066  van Gastel N, Stegen S, Eelen G, Schoors S, Carlier A, Daniëls VW, Baryawno N, Przybylski D, Depypere M, Stiers PJ, Lambrechts D, Van Looveren R, Torrekens S, Sharda A, Agostinis P, Lambrechts D, Maes F, Swinnen JV, Geris L, Van Oosterwyck H, Thienpont B, Carmeliet P, Scadden DT, Carmeliet G.  Lipid availability determines fate of skeletal progenitor cells via SOX9.  Nature  2020    PubMed ID: 32103177   DOI: 10.1038/s41586-020-2050-1
13232  Sakai S, Yoshii A, Sakurai S, Horii K, Nagasuna O.  Silk fibroin nanofibers: a promising ink additive for extrusion three-dimensional bioprinting.  Mater Today Bio  2020    PubMed ID: 33083780   DOI: 10.1016/j.mtbio.2020.100078
13376  Rico P, Rodrigo-Navarro A, Sánchez Pérez L, Salmeron-Sanchez M.  Borax induces osteogenesis by stimulating NaBC1 transporter via activation of BMP pathway.  Commun Biol  2020    PubMed ID: 33247189   DOI: 10.1038/s42003-020-01449-4
13428  J.A.Lenis, P.Rico, J.L. GómezRibelles, M.A.Pacha-Olivenza, M.L.González-Martín, F.J.Bolívar  Structure, morphology, adhesion and in vitro biological evaluation of antibacterial multi-layer HA-Ag/SiO2/TiN/Ti coatings obtained by RF magnetron sputtering for biomedical applications  Materials Science and Engineering:  2020    PubMed ID: 32806245   DOI: 10.1016/j.msec.2020.111268
13857  Gantumur E, Nakahata M, Kojima M, Sakai S.  Extrusion-Based Bioprinting through Glucose-Mediated Enzymatic Hydrogelation  Int J Bioprint  2020  6(1):250  PubMed ID: 32596552   DOI: 10.18063/ijb.v6i1.250
14438  Kazuki Y, Uno N, Abe S, Kajitani N, Kazuki K, Yakura Y, Sawada C, Takata S, Sugawara M, Nagashima Y, Okada A, Hiratsuka M, Osaki M, Ferrari G, Tedesco FS, Nishikawa S, Fukumoto K, Takayanagi SI, Kunisato A, Kaneko S, Oshimura M, Tomizuka K.  Engineering of human induced pluripotent stem cells via human artificial chromosome vectors for cell therapy and disease modeling  Mol Ther Nucleic Acids  2020  23:629-639  PubMed ID: 33552683   DOI: 10.1016/j.omtn.2020.12.012
11367  Ishijima Y, Ohmori S, Uneme A, Aoki Y, Kobori M, Ohida T, Arai M, Hosaka M, Ohneda K.  The Gata2 repression during 3T3-L1 preadipocyte differentiation is dependent on a rapid decrease in histone acetylation in response to glucocorticoid receptor activation.  Mol. Cell. Endocrinol.  2019    PubMed ID: 30615908   DOI: 10.1016/j.mce.2019.01.002
11397  Gantumur E, Sakai S, Nakahata M, Taya M.  Horseradish peroxidase-catalyzed hydrogelation consuming enzyme-produced hydrogen peroxide in the presence of reducing sugars.  Soft Matter  2019    PubMed ID: 30672948   DOI: 10.1039/c8sm01839a
11582  Numaga-Tomita T, Shimauchi T, Oda S, Tanaka T, Nishiyama K, Nishimura A, Birnbaumer L, Mori Y, Nishida M.  TRPC6 regulates phenotypic switching of vascular smooth muscle cells through plasma membrane potential-dependent coupling with PTEN.  FASEB J.  2019    PubMed ID: 31162976   DOI: 10.1096/fj.201802811R
11589  Aoki H, Yamashita M, Hashita T, Nakayama M, Yagi M, Iwao T, Matsunaga T.  Isolation of induced pluripotent stem cell-derived endothelial progenitor cells from sac-like structures.  Biochem. Biophys. Res. Commun.  2019  515(4):672-678  PubMed ID: 31178142   DOI: 10.1016/j.bbrc.2019.05.179
11681  Hiroe Morimoto-Ito, Masako Mizuno-Kamiya, Naoki Umemura, Yoshinori Inagaki, Eiji Takayama, Harumi Kawaki, Yasunori Muramatsu, Shinichiro Sumitomo, Nobuo Kondoh  Immunosuppressive Effect of Mesenchymal Stromal Cells is Enhanced by IL-1α from Oral Squamous Cell Carcinoma Cells  The Open Dentistry Journal  2019  13:221-227    DOI: 10.2174/1874210601913010221
11738  Iriguchi S, Kaneko S.  In Vitro Differentiation of T Cells: From Nonhuman Primate-Induced Pluripotent Stem Cells.  Methods Mol. Biol.  2019    PubMed ID: 31396934   DOI: 10.1007/978-1-4939-9728-2_11
11743  Kawai Y, Kaneko S.  Redifferentiation of Adaptive Naïve-Like CTL from T-Cell-Derived iPSC.  Methods Mol. Biol.  2019    PubMed ID: 31396930   DOI: 10.1007/978-1-4939-9728-2_7
11765  Abe T, Sato T, Yoda T, Hoshi K.  The period circadian clock 2 gene responds to glucocorticoids and regulates osteogenic capacity.  Regen Ther  2019    PubMed ID: 31489343   DOI: 10.1016/j.reth.2019.07.006
11838  Niimi K, Adachi Y, Ishikawa H, Yamaguchi W, Kubota Y, Inagaki S, Furuyama T.  Endothelial specific deletion of FOXO1 alters pericyte coverage in the developing retina.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31601422   DOI: 10.1016/j.bbrc.2019.10.040
6647  Takahata Y, Nakamura E, Hata K, Wakabayashi M, Murakami T, Wakamori K, Yoshikawa H, Matsuda A, Fukui N, Nishimura R.  Sox4 is involved in osteoarthritic cartilage deterioration through induction of ADAMTS4 and ADAMTS5.  FASEB J  2019  33(1):619-630  PubMed ID: 30016600   DOI: 10.1096/fj.201800259R
6878  Yano F, Ohba S, Murahashi Y, Tanaka S, Saito T, Chung UI.  Runx1 contributes to articular cartilage maintenance by enhancement of cartilage matrix production and suppression of hypertrophic differentiation.  Sci Rep  2019  9(1):7666  PubMed ID: 31113964   DOI: 10.1038/s41598-019-43948-3
6884  Iriguchi S, Kaneko S.  In Vitro Differentiation of T Cells: From Human Embryonic Stem Cells and Induced Pluripotent Stem Cells.  Methods Mol Biol  2019  2048:59-70  PubMed ID: 31396929   DOI: 10.1007/978-1-4939-9728-2_6
6907  Tagaya H, Ishikawa K, Hosokawa Y, Kobayashi S, Ueoka Y, Shimada M, Ohashi Y, Mikami H, Yamamoto M, Ihara T, Kumazawa K, Sugihara K, Goshima N, Watanabe S, Semba K.  A method of producing genetically manipulated mouse mammary gland.  Breast Cancer Res  2019  21(1):1  PubMed ID: 30611295   DOI: 10.1186/s13058-018-1086-8
9893  Zhongren Hu, Shurong Hong, Yu Zhang, Huijing Dai, Shuzhen Lin, Tingyu Yi, Hongmei Zhuang  Down-regulated WDR35 Contributes to Fetal Anomaly via Regulation of Osteogenic Differentiation  Gene  2019  20;697:48-56  PubMed ID: 30790652   DOI: 10.1016/j.gene.2019.02.034
12515  Baba TT, Miyazaki T, Ohara-Nemoto Y, Nemoto TK.  Suppressive effects of N-bisphosphonate in osteoblastic cells mitigated by non-N-bisphosphonate but not by sodium-dependent phosphate cotransporter inhibitor  Cell Biochem Funct  2019  37(6):400-407  PubMed ID: 31328801   DOI: 10.1002/cbf.3418
14881  J.A. Lenis, G. Bejarano, P. Rico, J.L. Gómez Ribelles, F.J. Bolívar  Development of multilayer Hydroxyapatite - Ag/TiN-Ti coatings deposited by radio frequency magnetron sputtering with potential application in the biomedical field  Surface and Coatings Technology  2019  377:124856    DOI: 10.1016/j.surfcoat.2019.06.097
10459  Kusuyama J, Kamisono A, ChangHwan S, Amir MS, Bandow K, Eiraku N, Ohnishi T, Matsuguchi T.  Spleen tyrosine kinase influences the early stages of multilineage differentiation of bone marrow stromal cell lines by regulating phospholipase C gamma activities.  J. Cell. Physiol.  2018  233:2549-2559  PubMed ID: 28786489   DOI: 10.1002/jcp.26130
11014    Versatility of hydrogelation by dual-enzymatic reactions with oxidases and peroxidase  Biochemical Engineering Journal  2018  131, 1-8    DOI: 10.1016/j.bej.2017.12.003
11047    Suppressive effect of mesenchymal stromal cells on interferon-γ-producing capability of spleen cells was specifically enhanced through humoral mediator(s) from mouse oral squamous cell carcinoma Sq-1979 Cells In Vitro  Cancer Translational Medicine  2018  4, 9-16    DOI: 10.4103/ctm.ctm_34_17
11132  Ito Y, Nakamura S, Sugimoto N, Shigemori T, Kato Y, Ohno M, Sakuma S, Ito K, Kumon H, Hirose H, Okamoto H, Nogawa M, Iwasaki M, Kihara S, Fujio K, Matsumoto T, Higashi N, Hashimoto K, Sawaguchi A, Harimoto KI, Nakagawa M, Yamamoto T, Handa M, Watanabe N, Nishi E, Arai F, Nishimura S, Eto K.  Turbulence Activates Platelet Biogenesis to Enable Clinical Scale Ex Vivo Production.  Cell  2018  174(3):636-648  PubMed ID: 30017246   DOI: 10.1016/j.cell.2018.06.011
11124  Kida J, Hata K, Nakamura E, Yagi H, Takahata Y, Murakami T, Maeda Y, Nishimura R.  Interaction of LEF1 with TAZ is necessary for the osteoblastogenic activity of Wnt3a.  Sci Rep  2018  8(1):10375  PubMed ID: 29991769   DOI: 10.1038/s41598-018-28711-4
11182  Sakai S, Mochizuki K, Qu Y, Mail M, Nakahata M, Taya M.  Peroxidase-catalyzed microextrusion bioprinting of cell-laden hydrogel constructs in vaporized ppm-level hydrogen peroxide.  Biofabrication  2018  10(4):045007  PubMed ID: 30137024   DOI: 10.1088/1758-5090/aadc9e
11138  Fukuda M, Yoshizawa T, Karim MF, Sobuz SU, Korogi W, Kobayasi D, Okanishi H, Tasaki M, Ono K, Sawa T, Sato Y, Chirifu M, Masuda T, Nakamura T, Tanoue H, Nakashima K, Kobashigawa Y, Morioka H, Bober E, Ohtsuki S, Yamagata Y, Ando Y, Oike Y, Araki N, Takeda S, Mizuta H, Yamagata K.  SIRT7 has a critical role in bone formation by regulating lysine acylation of SP7/Osterix.  Nat Commun  2018  9(1):2833  PubMed ID: 30026585   DOI: 10.1038/s41467-018-05187-4
4059  Yoshida K, Teramachi J, Uchibe K, Ikegame M, Qiu L, Yang D, Okamura H.  Reduction of protein phosphatase 2A Cα promotes in vivo bone formation and adipocyte differentiation.  Mol. Cell. Endocrinol.  2018  470:251-258  PubMed ID: 29128580   DOI: 10.1016/j.mce.2017.11.005
4109  Kiyama R, Nonoyama T, Wada S, Semba S, Kitamura N, Nakajima T, Kurokawa T, Yasuda K, Tanaka S, Gong JP.  Micro patterning of hydroxyapatite by soft lithography on hydrogels for selective osteoconduction.  Acta Biomater  2018    PubMed ID: 30292679   DOI: 10.1016/j.actbio.2018.10.002
4110  Takei H, Edahiro Y, Mano S, Masubuchi N, Mizukami Y, Imai M, Morishita S, Misawa K, Ochiai T, Tsuneda S, Endo H, Nakamura S, Eto K, Ohsaka A, Araki M, Komatsu N.  Skewed megakaryopoiesis in human induced pluripotent stem cell-derived haematopoietic progenitor cells harbouring calreticulin mutations.  Br. J. Haematol.  2018    PubMed ID: 29741776   DOI: 10.1111/bjh.15266
9466  Tetsuya Kawane 1, Xin Qin, Qing Jiang, Toshihiro Miyazaki, Hisato Komori, Carolina Andrea Yoshida, Viviane Keiko Dos Santos Matsuura-Kawata, Chiharu Sakane, Yuki Matsuo, Kazuhiro Nagai, Takafumi Maeno, Yuki Date, Riko Nishimura, Toshihisa Komori   Runx2 Is Required for the Proliferation of Osteoblast Progenitors and Induces Proliferation by Regulating Fgfr2 and Fgfr3  Sci Rep  2018  10;8(1):13551  PubMed ID: 30202094   DOI: 10.1038/s41598-018-31853-0
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18675  Hiraga T, Kizaka-Kondoh S, Hirota K, Hiraoka M, Yoneda T.  Hypoxia and hypoxia-inducible factor-1 expression enhance osteolytic bone metastases of breast cancer  Cancer Res  2007  67(9):4157-63  PubMed ID: 17483326   DOI: 10.1158/0008-5472.CAN-06-2355
18886  Zeng L, Kizaka-Kondoh S, Itasaka S, Xie X, Inoue M, Tanimoto K, Shibuya K, Hiraoka M.  Hypoxia inducible factor-1 influences sensitivity to paclitaxel of human lung cancer cell lines under normoxic conditions  Cancer Sci  2007  98(9):1394-401.  PubMed ID: 17608771   DOI: 10.1111/j.1349-7006.2007.00537.x
19265  Yamashiro T, Zheng L, Shitaku Y, Saito M, Tsubakimoto T, Takada K, Takano-Yamamoto T, Thesleff I.  Wnt10a regulates dentin sialophosphoprotein mRNA expression and possibly links odontoblast differentiation and tooth morphogenesis  Differentiation  2007  75(5):452-62  PubMed ID: 17286598   DOI: 10.1111/j.1432-0436.2006.00150.x
3584  Muramatsu S, Wakabayashi M, Ohno T, Amano K, Ooishi R, Sugahara T, Shiojiri S, Tashiro K, Suzuki Y, Nishimura R, Kuhara S, Sugano S, Yoneda T, Matsuda A.  Functional gene screening system identified TRPV4 as a regulator of chondrogenic differentiation.  J Biol Chem  2007  282(44):32158-67  PubMed ID: 17804410   DOI: 10.1074/jbc.M706158200
7246  Maruyama Z, Yoshida CA, Furuichi T, Amizuka N, Ito M, Fukuyama R, Miyazaki T, Kitaura H, Nakamura K, Fujita T, Kanatani N, Moriishi T, Yamana K, Liu W, Kawaguchi H, Nakamura K, Komori T.  Runx2 determines bone maturity and turnover rate in postnatal bone development and is involved in bone loss in estrogen deficiency.  Dev Dyn  2007  236(7):1876-90  PubMed ID: 17497678   DOI: 10.1002/dvdy.21187
7390  Iemata M, Takarada T, Hinoi E, Taniura H, Yoneda Y.  Suppression by glutamate of proliferative activity through glutathione depletion mediated by the cystine/glutamate antiporter in mesenchymal C3H10T1/2 stem cells.  J Cell Physiol  2007  213(3):721-9  PubMed ID: 17520696   DOI: 10.1002/jcp.21145
13955  Nakagawa K, Imai Y, Ohta Y, Takaoka K.  Prostaglandin E2 EP4 agonist (ONO-4819) accelerates BMP-induced osteoblastic differentiation  Bone  2007  41(4):543-8  PubMed ID: 17681894   DOI: 10.1016/j.bone.2007.06.013
3357  Hiroyama, Takashi, Miharada, Kenichi, Aoki, Naoko, Fujioka, Tsuyoshi, Sudo, Kazuhiro, Danjo, Inaho, Nagasawa, Toshiro, Nakamura, Yukio  Long-lasting in vitro hematopoiesis derived from primate embryonic stem cells.  Exp Hematol  2006  34:760-9  PubMed ID: 16728281   DOI: 10.1016/j.exphem.2006.03.004
19092  Yamagishi N, Tokunaga S, Ishihara K, Saito Y, Hatayama T.  The phenylic hydroxyl group is essential for the induction of stress response by sodium salicylate  Biochem Biophys Res Commun  2006  350(1):131-7  PubMed ID: 16996033   DOI: 10.1016/j.bbrc.2006.09.008
6998  Kawai S, Kato T, Sato M, Amano A.  Odd-skipped related 2 gene transcription is regulated by CCAAT enhancer-binding protein delta in mesenchymal C3H10T1/2 cells.  Genes Cells  2006  11(2):163-75  PubMed ID: 16436053   DOI: 10.1111/j.1365-2443.2006.00929.x
7004  Hirao M, Tamai N, Tsumaki N, Yoshikawa H, Myoui A.  Oxygen tension regulates chondrocyte differentiation and function during endochondral ossification.  J Biol Chem  2006  281(41):31079-92  PubMed ID: 16905540   DOI: 10.1074/jbc.M602296200
12831  Uozumi Y, Ito T, Hoshino Y, Mohri T, Maeda M, Takahashi K, Fujio Y, Azuma J.  Myogenic differentiation induces taurine transporter in association with taurine-mediated cytoprotection in skeletal muscles  Biochem J  2006  394(Pt 3):699-706  PubMed ID: 16318624   DOI: 10.1042/BJ20051303
3159  Miharada, Ken-ichi, Hiroyama, Takashi, Sudo, Kazuhiro, Nagasawa, Toshiro, Nakamura, Yukio  Lipocalin 2 functions as a negative regulator of red blood cell production in an autocrine fashion.  FASEB J  2005  19:1881-3  PubMed ID: 16157692   DOI: 10.1096/fj.05-3809fje
2874  Yano F, Kugimiya F, Ohba S, Ikeda T, Chikuda H, Ogasawara T, Ogata N, Takato T, Nakamura K, Kawaguchi H, Chung UI  The canonical Wnt signaling pathway promotes chondrocyte differentiation in a Sox9-dependent manner.  Biochem Biophys Res Commun  2005  333:1300-8  PubMed ID: 15979579   DOI: 10.1016/j.bbrc.2005.06.041
2915  Nobta M, Tsukazaki T, Shibata Y, Xin C, Moriishi T, Sakano S, Shindo H, Yamaguchi A  Critical regulation of bone morphogenetic protein-induced osteoblastic differentiation by Delta1/Jagged1-activated Notch1 signaling.  J Biol Chem  2005  280:15842-8  PubMed ID: 15695512   DOI: 10.1074/jbc.M412891200
2936  Kawai S, Kato T, Inaba H, Okahashi N, Amano A  Odd-skipped related 2 splicing variants show opposite transcriptional activity.  Biochem Biophys Res Commun  2005  328:306-11  PubMed ID: 15670784   DOI: 10.1016/j.bbrc.2004.12.173
3183  Hata, Kenji, Nishimura, Riko, Ueda, Mio, Ikeda, Fumiyo, Matsubara, Takuma, Ichida, Fumitaka, Hisada, Kunihiro, Nokubi, Takashi, Yamaguchi, Akira, Yoneda, Toshiyuki  A CCAAT/enhancer binding protein beta isoform, liver-enriched inhibitory protein, regulates commitment of osteoblasts and adipocytes.  Mol Cell Biol  2005  25:1971-9  PubMed ID: 15713650   DOI: 10.1128/MCB.25.5.1971-1979.2005
6876  Imai Y, Terai H, Nomura-Furuwatari C, Mizuno S, Matsumoto K, Nakamura T, Takaoka K.  Hepatocyte growth factor contributes to fracture repair by upregulating the expression of BMP receptors.  J Bone Miner Res  2005  20(10):1723-30  PubMed ID: 16160730   DOI: 10.1359/JBMR.050607
3158  Nishimura, Haruki, Nishimura, Masahiro, Oda, Ryo, Yamanaka, Katsuyuki, Matsubara, Takehiro, Ozaki, Yoshie, Sekiya, Kensuke, Hamada, Taizo, Kato, Yukio  Lectins induce resistance to proteases and/or mechanical stimulus in all examined cells--including bone marrow mesenchymal stem cells--on various scaffolds.  Exp Cell Res  2004  295:119-27  PubMed ID: 15051495   DOI: 10.1016/j.yexcr.2003.12.018
2893  Ichida, Fumitaka, Nishimura, Riko, Hata, Kenji, Matsubara, Takuma, Ikeda, Fumiyo, Hisada, Kunihiro, Yatani, Hirofumi, Cao, Xu, Komori, Toshihisa, Yamaguchi, Akira, Yoneda, Toshiyuki  Reciprocal roles of MSX2 in regulation of osteoblast and adipocyte differentiation.  J Biol Chem  2004  279:34015-22  PubMed ID: 15175325   DOI: 10.1074/jbc.M403621200
2899  Fujita, Takashi, Azuma, Yasutaka, Fukuyama, Ryo, Hattori, Yuji, Yoshida, Carolina, Koida, Masao, Ogita, Kiyokazu, Komori, Toshihisa  Runx2 induces osteoblast and chondrocyte differentiation and enhances their migration by coupling with PI3K-Akt signaling.  J Cell Biol  2004  166:85-95  PubMed ID: 15226309   DOI: 10.1083/jcb.200401138
3343  Ishihara, Keiichi, Yamagishi, Nobuyuki, Hatayama, Takumi  Suppression of heat- and polyglutamine-induced cytotoxicity by nonsteroidal anti-inflammatory drugs.  Eur J Biochem  2004  271:4552-8  PubMed ID: 15560796   DOI: 10.1111/j.1432-1033.2004.04419.x
6714  Date T, Doiguchi Y, Nobuta M, Shindo H.  Bone morphogenetic protein-2 induces differentiation of multipotent C3H10T1/2 cells into osteoblasts, chondrocytes, and adipocytes in vivo and in vitro.  J Orthop Sci  2004  9(5):503-8  PubMed ID: 15449126   DOI: 10.1007/s00776-004-0815-2
2644  Funato N, Ohyama K, Kuroda T, Nakamura M  Basic helix-loop-helix transcription factor epicardin/capsulin/Pod-1 suppresses differentiation by negative regulation of transcription  J Biol Chem  2003  278:7486-7493  PubMed ID: 12493738   DOI: 10.1074/jbc.M212248200
2635  Hata K, Nishimura R, Ikeda F, Yamashita K, Matsubara T, Nokubi T, Yoneda T  Differential roles of Smad1 and p38 kinase in regulation of peroxisome proliferator-activating receptor gamma during bone morphogenetic protein 2-induced adipogenesis.  Mol Biol Cell  2003  14:545-55  PubMed ID: 12589053   DOI: 10.1091/mbc.E02-06-0356
4944  Oda R, Suardita K, Fujimoto K, Pan H, Yan W, Shimazu A, Shintani H, Kato Y.  Anti-membrane-bound transferrin-like protein antibodies induce cell-shape change and chondrocyte differentiation in the presence or absence of concanavalin A.  J Cell Sci  2003  116(Pt 10):2029-38  PubMed ID: 12679380   DOI: 10.1242/jcs.00393
5039  Murata T, Sugatani T, Shimizu K, Manganiello VC, Tagawa T.  Effect of the phosphodiesterase 4 inhibitor, rolipram, on retinoic acid-increased alkaline phosphatase activity in the mouse fibroblastic C3H10T1/2 cell line.  Arch Oral Biol  2003  48(1):63-7  PubMed ID: 12615143   DOI: 10.1016/s0003-9969(02)00166-8
3557  Tezuka K, Yasuda M, Watanabe N, Morimura N, Kuroda K, Miyatani S, Hozumi N.  Stimulation of osteoblastic cell differentiation by Notch.  J Bone Miner Res  2002  17(2):231-9  PubMed ID: 11811553   DOI: 10.1359/jbmr.2002.17.2.231
3560  Fujita T, Meguro T, Fukuyama R, Nakamuta H, Koida M.  New signaling pathway for parathyroid hormone and cyclic AMP action on extracellular-regulated kinase and cell proliferation in bone cells. Checkpoint of modulation by cyclic AMP.  J Biol Chem  2002  277(25):22191-200  PubMed ID: 11956184   DOI: 10.1074/jbc.M110364200
3566  Suardita K, Fujimoto K, Oda R, Shimazu A, Miyazaki K, Kawamoto T, Kato Y.  Effects of overexpression of membrane-bound transferrin-like protein (MTf) on chondrogenic differentiation in Vitro.  J Biol Chem  2002  277(50):48579-86  PubMed ID: 12374788   DOI: 10.1074/jbc.M209243200
6691  Yuasa T, Kataoka H, Kinto N, Iwamoto M, Enomoto-Iwamoto M, Iemura S, Ueno N, Shibata Y, Kurosawa H, Yamaguchi A.  Sonic hedgehog is involved in osteoblast differentiation by cooperating with BMP-2.  J Cell Physiol  2002  193(2):225-32  PubMed ID: 12385000   DOI: 10.1002/jcp.10166
6699  Tang L, Ebara S, Kawasaki S, Wakabayashi S, Nikaido T, Takaoka K.  FK506 enhanced osteoblastic differentiation in mesenchymal cells.  Cell Biol Int  2002  26(1):75-84  PubMed ID: 11779223   DOI: 10.1006/cbir.2001.0812
6703  Katagiri T, Imada M, Yanai T, Suda T, Takahashi N, Kamijo R.  Identification of a BMP-responsive element in Id1, the gene for inhibition of myogenesis.  Genes Cells  2002  7(9):949-60  PubMed ID: 12296825   DOI: 10.1046/j.1365-2443.2002.00573.x
2480  Maeda T, Abe M, Kurisu K, Jikko A, Furukawa S  Molecular cloning and characterization of a novel gene, CORS26, encoding a putative secretory protein and its possible involvement in skeletal development.  J Biol Chem  2001  276:3628-34  PubMed ID: 11071891   DOI: 10.1074/jbc.M007898200
6157  Muramatsu M, Hanazono Y, Ogasawara Y, Okada T, Mizukami H, Kume A, Mizoguchi H, Ozawa K.  Reversible integration of the dominant negative retinoid receptor gene for ex vivo expansion of hematopoietic stem/progenitor cells.  Biochem Biophys Res Commun  2001  285(4):891-6  PubMed ID: 11467834   DOI: 10.1006/bbrc.2001.5264
3542  Ito H, Akiyama H, Iguchi H, Iyama K, Miyamoto M, Ohsawa K, Nakamura T.  Molecular cloning and biological activity of a novel lysyl oxidase-related gene expressed in cartilage.  J Biol Chem  2001  276(26):24023-9  PubMed ID: 11292829   DOI: 10.1074/jbc.M100861200
6659  Tamura S, Kataoka H, Matsui Y, Shionoya Y, Ohno K, Michi KI, Takahashi K, Yamaguchi A.  The effects of transplantation of osteoblastic cells with bone morphogenetic protein (BMP)/carrier complex on bone repair.  Bone  2001  29(2):169-75  PubMed ID: 11502479   DOI: 10.1016/s8756-3282(01)00498-7
16961  Hamada T, Ui-Tei K, Imaki J, Miyata Y.  Molecular cloning of SCDGF-B, a novel growth factor homologous to SCDGF/PDGF-C/fallotein  Biochem Biophys Res Commun  2001  280(3):733-7  PubMed ID: 11162582   DOI: 10.1006/bbrc.2000.4187
3989  Yoshimasa FUJIMAKI  The Role of Recombinant Human Bone Morphogenetic Protein 2 (rhBMP-2) in the Differentiation of Osteoblasts and Chondrocytes  The Showa University Journal of Medical Sciences  2000  12:39-49    DOI: 10.15369/sujms1989.12.39
3997  Enomoto-Iwamoto M, Nakamura T, Aikawa T, Higuchi Y, Yuasa T, Yamaguchi A, Nohno T, Noji S, Matsuya T, Kurisu K, Koyama E, Pacifici M, Iwamoto M.  Hedgehog proteins stimulate chondrogenic cell differentiation and cartilage formation.  J Bone Miner Res  2000  15(9):1659-68  PubMed ID: 10976986   DOI: 10.1359/jbmr.2000.15.9.1659
3540  Kusanagi K, Inoue H, Ishidou Y, Mishima HK, Kawabata M, Miyazono K.  Characterization of a bone morphogenetic protein-responsive Smad-binding element.  Mol Biol Cell  2000  11(2):555-65  PubMed ID: 10679014   DOI: 10.1091/mbc.11.2.555
17277  Nakane T, Inada Y, Ito F, Itoh N, Tazawa S, Chiba S.  Cloning and expression of mouse deafness dystonia peptide 1 cDNA  Biochem Biophys Res Commun  2000  273(2):759-64  PubMed ID: 10873677   DOI: 10.1006/bbrc.2000.3004
3969  Akiyama H1, Hiraki Y, Noda M, Shigeno C, Ito H, Nakamura T.  Molecular cloning and biological activity of a novel Ha-Ras suppressor gene predominantly expressed in skeletal muscle, heart, brain, and bone marrow by differential display using clonal mouse EC cells, ATDC5.  J Biol Chem  1999.  274(45):32192-7  PubMed ID: 10542256   DOI: 10.1074/jbc.274.45.32192
4831  Kume A1, Ito K, Ueda Y, Hasegawa M, Urabe M, Mano H, Ozawa K.  A G-CSF receptor-gyrase B fusion gene: A new type of molecular switch for expansion of genetically modified hematopoietic cells.  Biochem Biophys Res Commun  1999  260(1):9-12  PubMed ID: 10381335   DOI: 10.1006/bbrc.1999.0859
4833  Matsuda KM, Kume A, Ueda Y, Urabe M, Hasegawa M, Ozawa K.  Development of a modified selective amplifier gene for hematopoietic stem cell gene therapy  Gene Ther  1999  6(6):1038-44  PubMed ID: 10455406   DOI: 10.1038/sj.gt.3300906
3968  Tamura M, Noda M.  Identification of DERMO-1 as a member of helix-loop-helix type transcription factors expressed in osteoblastic cells.  J Cell Biochem  1999  72(2):167-76  PubMed ID: 10022499   DOI: 10.1002/(sici)1097-4644(19990201)72:2<167::aid-jcb1>3.0.co;2-3
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3922  Yanaka N, Akatsuka H, Kawai E, Omori K.  1,25-Dihydroxyvitamin D3 upregulates natriuretic peptide receptor-C expression in mouse osteoblasts.  Am J Physiol  1998  275(6):E965-73  PubMed ID: 9843738   DOI: 10.1152/ajpendo.1998.275.6.E965
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3808  Kinto N, Iwamoto M, Enomoto-Iwamoto M, Noji S, Ohuchi H, Yoshioka H, Kataoka H, Wada Y, Yuhao G, Takahashi HE, Yoshiki S, Yamaguchi A.  Fibroblasts expressing Sonic hedgehog induce osteoblast differentiation and ectopic bone formation.  FEBS Lett  1997  404(2-3):319-23  PubMed ID: 9119087   DOI: 10.1016/s0014-5793(97)00014-8
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15576  Katagiri T, Akiyama S, Namiki M, Komaki M, Yamaguchi A, Rosen V, Wozney JM, Fujisawa-Sehara A, Suda T.  Bone morphogenetic protein-2 inhibits terminal differentiation of myogenic cells by suppressing the transcriptional activity of MyoD and myogenin  Exp Cell Res  1997  230(2):342-51  PubMed ID: 9024793   DOI: 10.1006/excr.1996.3432
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