Cell No. : Cell Name RCB0245 : Nil1C1  update : 2023/06/30
Comment			A fibroblastic cell line derived from a fetus of Syrian hamster. Transformed cells. Tumor formation and metastasis can be observed after transplantation, but these abilities are much lower than Nil2C2.
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Sakiyama, Hisako
		Originator	Diamond
		Year of deposit	1988
		Animal	_hamster < Mammals
		Age at sampling	embryo
		Tissue	embryo
		Classification	cancer
		Lifespan	infinite
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_8737
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		MEM + 10% CS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 8 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma		(-)
		Animal PCR		OK
		Isozyme		LD, NP
Reference information		Reference	6
		User's Publication	1

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Reference

20  Sakiyama H, Terasima T, Sato K.  Effects of confluent monolayers of density-inhibited and -transformed cells on the growth of superinoculated cells  Cancer Res  1978  38(9):2854-8  PubMed ID: 679192

2776  Sakiyama H, Terasima T  The synthesis of Forssman glycolipid in clones of nil 2 hamsters fibroblasts grown in monolayer or spinner culture.  Cancer Res  1975  35:1723-6  PubMed ID: 1169114

2775  Sakiyama H, Robbins PW  Effect of transformation by hamster sarcoma virus on the glycolipid composition of secondary hamster embryo cells and the Nil cell line.  In Vitro  1974  9:331-8  PubMed ID: 4856904   DOI: 10.1007/BF02616016

2774  Sakiyama H, Robbins PW  The effect of dibutyryl adenosine 3':5'-cyclic monophosphate on the synthesis of glycolipids by normal and transformed Nil cells.  Arch Biochem Biophys  1973  154:407-14  PubMed ID: 4347686   DOI: 10.1016/0003-9861(73)90073-8

1074  Sakiyama H, Gross, S K, Robbins, P W  Glycolipid synthesis in normal and virus-transformed hamster cell lines.  Proc Natl Acad Sci U S A  1972  69:872-6  PubMed ID: 4337244   DOI: 10.1073/pnas.69.4.872

2080  Diamond L  Two spontaneously transformed cell lines derived from the same hamster embryo culture.  Int J Cancer  1967  2:143-52  PubMed ID: 6039764   DOI: 10.1002/ijc.2910020209

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User's Publication

2773  Sakiyama H, Nonaka T, Masuda R, Inoue N, Kuboki Y, Iijima M, Hirabayasi Y, Takahagi M, Yoshida K, Kuriiwa K, Yoshida M, Imajoh-Ohmi S  Characterization of mineral deposits formed in cultures of a hamster tartrate-resistant acid phosphatase (TRAP) and alkaline phosphatase (ALP) double-positive cell line (CCP).  Cell Tissue Res  2002  309:269-79  PubMed ID: 12172786   DOI: 10.1007/s00441-002-0587-y