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Cell No. : Cell Name
RCB0244 : Nil2C1  update : 2022/03/08
CommentA fibroblastic cell line derived from a fetus of Syrian hamster. Transformed cells. Tumor formation and metastasis can be observed after transplantation, but these abilities are lower than Nil2C2.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sakiyama, Hisako
Originator Diamond
Year of deposit 1988
Animal hamster < Mammals
Age at sampling embryo
Tissue embryo
Classification cancer
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_8739
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 5
User's Publication 0

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19  Sakiyama H, Otsu H, Kanegasaki S.  Cellular interaction between fixed and living cells. Transfer of radioactive materials from living cells to fixed cells  J Cell Biol  1979  81(3):498-509  PubMed ID: 379019   DOI: 10.1083/jcb.81.3.498
20  Sakiyama H, Terasima T, Sato K.  Effects of confluent monolayers of density-inhibited and -transformed cells on the growth of superinoculated cells  Cancer Res  1978  38(9):2854-8  PubMed ID: 679192  
2775  Sakiyama H, Robbins PW  Effect of transformation by hamster sarcoma virus on the glycolipid composition of secondary hamster embryo cells and the Nil cell line.  In Vitro  1974  9:331-8  PubMed ID: 4856904   DOI: 10.1007/BF02616016
2774  Sakiyama H, Robbins PW  The effect of dibutyryl adenosine 3':5'-cyclic monophosphate on the synthesis of glycolipids by normal and transformed Nil cells.  Arch Biochem Biophys  1973  154:407-14  PubMed ID: 4347686   DOI: 10.1016/0003-9861(73)90073-8
1074  Sakiyama H, Gross, S K, Robbins, P W  Glycolipid synthesis in normal and virus-transformed hamster cell lines.  Proc Natl Acad Sci U S A  1972  69:872-6  PubMed ID: 4337244   DOI: 10.1073/pnas.69.4.872

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