Cell No. : Cell Name
RCB0244 : Nil2C1
update : 2024/11/14
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| Comment | A fibroblastic cell line derived from a fetus of Syrian hamster. Transformed cells. Tumor formation and metastasis can be observed after transplantation, but these abilities are lower than Nil2C2. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
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|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
Sakiyama, Hisako
|
| Originator |
Diamond
|
| Year of deposit |
1988
|
| Animal |
_hamster
< Mammals
|
| Age at sampling |
embryo
|
| Tissue |
embryo
|
| Classification |
cancer
|
|
Lifespan |
infinite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_8739
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
MEM + 10% CS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 8 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Animal PCR |
|
OK
|
| Isozyme |
|
LD, NP
|
| Reference information |
Reference |
5
|
| User's Publication |
0
|
| Reference |
|
19
Sakiyama H, Otsu H, Kanegasaki S.
Cellular interaction between fixed and living cells. Transfer of radioactive materials from living cells to fixed cells
J Cell Biol
1979
81(3):498-509
PubMed ID: 379019
DOI: 10.1083/jcb.81.3.498
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20
Sakiyama H, Terasima T, Sato K.
Effects of confluent monolayers of density-inhibited and -transformed cells on the growth of superinoculated cells
Cancer Res
1978
38(9):2854-8
PubMed ID: 679192
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2775
Sakiyama H, Robbins PW
Effect of transformation by hamster sarcoma virus on the glycolipid composition of secondary hamster embryo cells and the Nil cell line.
In Vitro
1974
9:331-8
PubMed ID: 4856904
DOI: 10.1007/BF02616016
|
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2774
Sakiyama H, Robbins PW
The effect of dibutyryl adenosine 3':5'-cyclic monophosphate on the synthesis of glycolipids by normal and transformed Nil cells.
Arch Biochem Biophys
1973
154:407-14
PubMed ID: 4347686
DOI: 10.1016/0003-9861(73)90073-8
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1074
Sakiyama H, Gross, S K, Robbins, P W
Glycolipid synthesis in normal and virus-transformed hamster cell lines.
Proc Natl Acad Sci U S A
1972
69:872-6
PubMed ID: 4337244
DOI: 10.1073/pnas.69.4.872
|
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