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Cell No. : Cell Name
RCB0243 : Nil2C2  update : 2023/06/30
CommentA fibroblastic cell line derived from a fetus of Syrian hamster. Transformed cells. Tumor formation and metastasis can be observed after transplantation.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor Sakiyama, Hisako
Originator Diamond
Year of deposit 1988
Animal _hamster < Mammals
Age at sampling embryo
Tissue embryo
Classification cancer
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_8740
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Culture medium MEM + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Animal PCR OK
Isozyme LD, NP
Reference information Reference 11
User's Publication 0

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16  Nakagawa K, Sakiyama H, Fukazawa T, Matsumoto M, Takigawa M, Toyoguchi T, Moriya H.  Coordinated change between complement C1s production and chondrocyte differentiation in vitro  Cell Tissue Res  1997  289(2):299-305  PubMed ID: 9211832   DOI: 10.1007/s004410050876
2779  Yamaguchi K, Kato N, Sakai N, Matsumoto M, Nagasawa S, Hatsuse H, Toyoguchi T, Moriya H, Sakiyama H  Immune complex independent activation of complement, C1s secreted from hamster embryo malignant fibroblasts, Nil2C2 in serum free culture medium.  Biochim Biophys Acta  1994  1205:133-8  PubMed ID: 8142477   DOI: 10.1016/0167-4838(94)90101-5
2082  Sakiyama H, Yamaguchi K, Chiba K, Nagata K, Taniyama C, Matsumoto M, Suzuki G, Tanaka T, Tomoasawa T, Yasukawa M, et al.  Biochemical characterization and tissue distribution of hamster complement C1s.  J Immunol  1991  146:183-7  PubMed ID: 1898597  
2778  Sakiyama H, Nishino Y, Tanaka T, Tomosawa T, Kinoshita H, Nagata K, Chiba K, Sakiyama S  Purification and characterization of a novel calcium-dependent serine proteinase secreted from malignant hamster embryo fibroblast Nil2C2.  Biochim Biophys Acta  1989  990:156-61  PubMed ID: 2917176   DOI: 10.1016/s0304-4165(89)80028-5
2081  Sakiyama H, Nishino Y, Nishimura K, Noda Y, Otsu H  Phagocytosis and solubilization of fixed cells by metastatic hamster embryo fibroblasts, Nil2C2.  Cancer Res  1984  44:2023-32  PubMed ID: 6370424  
2776  Sakiyama H, Terasima T  The synthesis of Forssman glycolipid in clones of nil 2 hamsters fibroblasts grown in monolayer or spinner culture.  Cancer Res  1975  35:1723-6  PubMed ID: 1169114  
2775  Sakiyama H, Robbins PW  Effect of transformation by hamster sarcoma virus on the glycolipid composition of secondary hamster embryo cells and the Nil cell line.  In Vitro  1974  9:331-8  PubMed ID: 4856904   DOI: 10.1007/BF02616016
2774  Sakiyama H, Robbins PW  The effect of dibutyryl adenosine 3':5'-cyclic monophosphate on the synthesis of glycolipids by normal and transformed Nil cells.  Arch Biochem Biophys  1973  154:407-14  PubMed ID: 4347686   DOI: 10.1016/0003-9861(73)90073-8
1075  Sakiyama H, Robbins, P W  Glycolipid synthesis and tumorigenicity of clones isolated from the Nil 2 line of hamster embryo fibroblasts.  Fed Proc  1973  32:86-90  PubMed ID: 4346327  
1074  Sakiyama H, Gross, S K, Robbins, P W  Glycolipid synthesis in normal and virus-transformed hamster cell lines.  Proc Natl Acad Sci U S A  1972  69:872-6  PubMed ID: 4337244   DOI: 10.1073/pnas.69.4.872
2080  Diamond L  Two spontaneously transformed cell lines derived from the same hamster embryo culture.  Int J Cancer  1967  2:143-52  PubMed ID: 6039764   DOI: 10.1002/ijc.2910020209

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