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Cell No. : Cell Name
RCB0218 : MRC-5  update : 2022/03/08
CommentNormal embryonic lung fibroblast with accurate PDL
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Ohno, Tadao
Originator Jacobs, J. P.
Year of deposit 1987
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 14 weeks embryo
Tissue embryo/fetus, lung
Classification normal
History Given directly from Dr. Jacobs
Lifespan finite
Crisis PDL 48< PDL
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_0440
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HF-RITC80-7 (or MEM ALPHA) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 42-47(50) : 42(3),43(3),44(6),45(12),46(24),47(2)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 48


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Reference
1028  Jacobs, J P, Jones, C M, Baille, J P  Characteristics of a human diploid cell designated MRC-5.  Nature  1970  227:168-70  PubMed ID: 4316953   DOI: 10.1038/227168a0

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User's Publication
20512  Shimura T, Nakashiro C, Fujiwara K, Shiga R, Sasatani M, Kamiya K, Ushiyama A.  Radiation affects glutathione redox reaction by reduced glutathione peroxidase activity in human fibroblasts.  J Radiat Res  2022    PubMed ID: 34977941   DOI: 10.1093/jrr/rrab122
20585  Yanagimura N, Takeuchi S, Fukuda K, Arai S, Tanimoto A, Nishiyama A, Ogo N, Takahashi H, Asai A, Watanabe S, Kikuchi T, Yano S.  STAT3 inhibition suppresses adaptive survival of ALK-rearranged lung cancer cells through transcriptional modulation of apoptosis.  NPJ Precis Oncol  2022  6(1):11  PubMed ID: 35228642   DOI: 10.1038/s41698-022-00254-y
16391  Sakamoto T, Yu Z, Otani Y.  Dual-Color Fluorescence Switch-On Probe for Imaging G-Quadruplex and Double-Stranded DNA in Living Cells  Anal Chem  2022  Mar 2  PubMed ID: 35234461   DOI: 10.1021/acs.analchem.1c04804
20770  Ito K, Nishida Y, Hamada S, Shimizu K, Sakai T, Ohkawara B, Alman BA, Enomoto A, Ikuta K, Koike H, Zhang J, Ohno K, Imagama S.  Efficacy of auranofin as an inhibitor of desmoid progression.  Sci Rep  2022  12(1):11918  PubMed ID: 35831372   DOI: 10.1038/s41598-022-15756-9
20785  Ju N, Hayashi H, Shimamura M, Baba S, Yoshida S, Morishita R, Rakugi H, Nakagami H.  Prevention of bleomycin-induced pulmonary fibrosis by a RANKL peptide in mice.  Sci Rep  2022  12(1):12474  PubMed ID: 35864207   DOI: 10.1038/s41598-022-16843-7
20843  Shimura T, Zaharieva E, Sasatani M, Kawai H, Kamiya K, Ushiyama A.  ACTIVATION OF HUMAN FIBROBLASTS BY CHRONIC RADIATION RATHER THAN ACUTE RADIATION.  Radiat Prot Dosimetry  2022  198(13-15):1098-1103  PubMed ID: 36083750   DOI: 10.1093/rpd/ncac065
20934  Shimura T, Totani R, Ogasawara H, Inomata K, Sasatani M, Kamiya K, Ushiyama A.  Effects of oxygen on the response of mitochondria to X-irradiation and reactive oxygen species-mediated fibroblast activation.  Int J Radiat Biol  2022  1-10  PubMed ID: 36383181   DOI: 10.1080/09553002.2023.2142980
20050  Yap JMG, Ueda T, Kanemitsu Y, Takeda N, Fukumitsu K, Fukuda S, Uemura T, Tajiri T, Ohkubo H, Maeno K, Ito Y, Oguri T, Ugawa S, Niimi A.  AITC inhibits fibroblast-myofibroblast transition via TRPA1-independent MAPK and NRF2/HO-1 pathways and reverses corticosteroids insensitivity in human lung fibroblasts.  Respir Res  2021  22(1):51  PubMed ID: 33579280   DOI: 10.1186/s12931-021-01636-9
20392  Shikata M, Toyooka T, Komaki Y, Ibuki Y.  4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone-Induced Histone Acetylation via α7nAChR-Mediated PI3K/Akt Activation and Its Impact on γ-H2AX Generation.  Chem Res Toxicol  2021    PubMed ID: 34784199   DOI: 10.1021/acs.chemrestox.1c00287
9839  Tomohito Yoshihara, Yasuhiro Nanri, Satoshi Nunomura, Yukie Yamaguchi, Carol Feghali-Bostwick, Keiichi Ajito, Shoichi Murakami 4, Masaaki Mawatari, Kenji Izuhara  Periostin Plays a Critical Role in the Cell Cycle in Lung Fibroblasts  Respir Res  2020  21(1):38  PubMed ID: 32000779   DOI: 10.1186/s12931-020-1299-0
12045  Yap JMG, Ueda T, Takeda N, Fukumitsu K, Fukuda S, Uemura T, Tajiri T, Ohkubo H, Maeno K, Ito Y, Kanemitsu Y, Niimi A.  An inflammatory stimulus sensitizes TRPA1 channel to increase cytokine release in human lung fibroblasts.  Cytokine  2020    PubMed ID: 32050145   DOI: 10.1016/j.cyto.2020.155027
13016  Sano M, Yada R, Nomura Y, Kusukawa T, Ando H, Matsumoto K, Wada K, Tanaka T, Ohara H, Aso Y.  Microbial Screening Based on the Mizoroki-Heck Reaction Permits Exploration of Hydroxyhexylitaconic-Acid-Producing Fungi in Soils.  Microorganisms  2020    PubMed ID: 32365722   DOI: 10.3390/microorganisms8050648
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11423  Fukuda K, Takeuchi S, Arai S, Katayama R, Nanjo S, Tanimoto A, Nishiyama A, Nakagawa T, Taniguchi H, Suzuki T, Yamada T, Nishihara H, Ninomiya H, Ishikawa Y, Baba S, Takeuchi K, Horiike A, Yanagitani N, Nishio M, Yano S.  Epithelial-to-Mesenchymal Transition Is a Mechanism of ALK Inhibitor Resistance in Lung Cancer Independent of ALK Mutation Status.  Cancer Res.  2019    PubMed ID: 30737231   DOI: 10.1158/0008-5472.CAN-18-2052
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6901  Terasaki M, Ikuta M, Kojima H, Tanaka T, Maeda H, Miyashita K, Mutoh M.  Dietary Fucoxanthin Induces Anoikis in Colorectal Adenocarcinoma by Suppressing Integrin Signaling in a Murine Colorectal Cancer Model.  J Clin Med  2019  9(1):90  PubMed ID: 31905803   DOI: 10.3390/jcm9010090
10822  Takashina T, Koyama T, Nohara S, Hasegawa M, Ishiguro A, Iijima K, Lu J, Shimura M, Okamura T, Sakuma T, Yamamoto T, Ishizaka Y.  Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering.  Biomaterials  2018  173:11-21  PubMed ID: 29734017   DOI: 10.1016/j.biomaterials.2018.04.040
11194  Park AM, Tsunoda I, Yoshie O.  Heat shock protein 27 promotes cell cycle progression by down-regulating E2F transcription factor 4 and retinoblastoma family protein p130.  J. Biol. Chem.  2018  293(41):15815-15826  PubMed ID: 30166342   DOI: 10.1074/jbc.RA118.003310
4305  Nakano T, Yamamura ET, Fujita H, Sone T, Asano K.  Novel methods for nucleotide length control in double-stranded polyinosinic-polycytidylic acid production using uneven length components.  Biosci. Biotechnol. Biochem.  2018    PubMed ID: 30079840   DOI: 10.1080/09168451.2018.1501264
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10135  Komiya K, Ohta S, Arima K, Ogawa M, Suzuki S, Mitamura Y, Nunomura S, Nanri Y, Yoshihara T, Kawaguchi A, Kadota JI, Rubin BK, Izuhara K.  Clarithromycin attenuates IL-13-induced periostin production in human lung fibroblasts.  Respir. Res.  2017  18:37  PubMed ID: 28219384   DOI: 10.1186/s12931-017-0519-8
10487  Adachi Y, Watanabe K, Kita K, Kitai H, Kotani H, Sato Y, Inase N, Yano S, Ebi H.  Resistance mediated by alternative receptor tyrosine kinases in FGFR1-amplified lung cancer.  Carcinogenesis  2017  38:1063-1072  PubMed ID: 28968756   DOI: 10.1093/carcin/bgx091
5446  Endo S1, Nakata K2, Ohuchida K3, Takesue S1, Nakayama H1, Abe T1, Koikawa K, Okumura T, Sada M, Horioka K, Zheng B, Mizuuchi Y, Iwamoto C5, Murata M, Moriyama T, Miyasaka Y, Ohtsuka T, Mizumoto K, Oda Y, Hashizume M, Nakamura M  Autophagy Is Required for Activation of Pancreatic Stellate Cells, Associated With Pancreatic Cancer Progression and Promotes Growth of Pancreatic Tumors in Mice.  Gastroenterology  2017  152(6):1492-1506  PubMed ID: 28126348   DOI: 10.1053/j.gastro.2017.01.010
14607  Nanjo S, Arai S, Wang W, Takeuchi S, Yamada T, Hata A, Katakami N, Okada Y, Yano S.  MET Copy Number Gain Is Associated with Gefitinib Resistance in Leptomeningeal Carcinomatosis of EGFR-mutant Lung Cancer  Mol Cancer Ther  2017  16(3):506-515  PubMed ID: 28138027   DOI: 10.1158/1535-7163.MCT-16-0522
12930  Park AM, Kanai K, Itoh T, Sato T, Tsukui T, Inagaki Y, Selman M, Matsushima K, Yoshie O.  Myofibroblast Differentiation and in the Development of Bleomycin-Induced Pulmonary Fibrosis  PLoS One  2016  11(2):e0148998  PubMed ID: 26859835   DOI: 10.1371/journal.pone.0148998
10168  Taku Chibazakura, Yui Toriyabe, Hiroshi Fujii, Kiwamu Takahashi, Mariko Kawakami, Haruna Kuwamura, Hazuki Haga, Shun-ichiro Ogura, Fuminori Abe, Motowo Nakajima, Hirofumi Yoshikawa, Tohru Tanaka  5-Aminolevulinic Acid Enhances Cell Death Under Thermal Stress in Certain Cancer Cell Lines  Biosci Biotechnol Biochem   2015  79(3):422-31  PubMed ID: 25346276   DOI: 10.1080/09168451.2014.975186
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8835  Yoshida K, Munakata H.  Connective tissue growth factor binds to fibronectin through the type I repeat modules and enhances the affinity of fibronectin to fibrin.  Biochim. Biophys. Acta  2007  1770:672-80  PubMed ID: 17239539   DOI: 10.1016/j.bbagen.2006.11.010
3413  Yamashita, Satoko, Katayama, Hideki, Saitoh, Hiroyuki, Akao, Tetsuyuki, Park, Yu Shin, Mizuki, Eiichi, Ohba, Michio, Ito, Akio  Typical Three-Domain Cry Proteins of Bacillus thuringiensis Strain A1462 Exhibit Cytocidal Activity on Limited Human Cancer Cells.  J Biochem  2005  138:663-72  PubMed ID: 16428294   DOI: 10.1093/jb/mvi177
2892  Katayama H, Yokota H, Akao T, Nakamura O, Ohba M, Mekada E, Mizuki E  Parasporin-1, a novel cytotoxic protein to human cells from non-insecticidal parasporal inclusions of Bacillus thuringiensis  J Biochem (Tokyo)  2005  137:17-25  PubMed ID: 15713879   DOI: 10.1093/jb/mvi003
3202  Ohuchida, Kenoki, Mizumoto, Kazuhiro, Ogura, Yasuhiro, Ishikawa, Nami, Nagai, Eishi, Yamaguchi, Koji, Tanaka, Masao  Quantitative assessment of telomerase activity and human telomerase reverse transcriptase messenger RNA levels in pancreatic juice samples for the diagnosis of pancreatic cancer.  Clin Cancer Res  2005  11:2285-92  PubMed ID: 15788678   DOI: 10.1158/1078-0432.CCR-04-1581
3434  Nikawa H, Egusa H, Makihira S, Okamoto T, Kurihara H, Shiba H, Amano H, Murayama T, Yatani H, Hamada T.  An in vitro evaluation of the adhesion of Candida species to oral and lung tissue cells.  Mycoses  2005  49:14-7  PubMed ID: 16367812   DOI: 10.1111/j.1439-0507.2005.01176.x
2894  Ito, Akio, Sasaguri, Yasuyuki, Kitada, Sakae, Kusaka, Yoshitomo, Kuwano, Kyoko, Masutomi, Kenjiro, Mizuki, Eiichi, Akao, Tetsuyuki, Ohba, Michio  A Bacillus thuringiensis crystal protein with selective cytocidal action to human cells.  J Biol Chem  2004  279:21282-6  PubMed ID: 15026424   DOI: 10.1074/jbc.M401881200
2672  Manabe, Tatsuya, Mizumoto, Kazuhiro, Nagai, Eishi, Matsumoto, Kunio, Nakamura, Toshikazu, Nukiwa, Toshihiro, Tanaka, Masao, Matsuda, Takehisa  Cell-based protein delivery system for the inhibition of the growth of pancreatic cancer: NK4 gene-transduced oral mucosal epithelial cell sheet.  Clin Cancer Res  2003  9:3158-66  PubMed ID: 12912968  
2709  Yagi, Yasuyuki, Sotani, Tomohiro, Nagao, Toshinori, Horio, Tomoyo, Yamamoto, Itaru, Gohda, Eiichi  Induction by staurosporine of hepatocyte growth factor production in human skin fibroblasts independent of protein kinase inhibition.  Biochem Pharmacol  2003  66:1797-808  PubMed ID: 14563490   DOI: 10.1016/s0006-2952(03)00547-1
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