Cell No. : Cell Name RCB0218 : MRC-5  update : 2024/06/12
Comment			Normal embryonic lung fibroblast with accurate PDL
Comment from the depositor
Terms and conditions			There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
	Basic information	Depositor	Ohno, Tadao
		Originator	Jacobs, J. P.
		Year of deposit	1987
		Animal	_human < Mammals
		Genus	Homo
		Species	sapiens
		Gender	Male
		Age at sampling	14 weeks embryo
		Tissue	embryo/fetus, lung
		Classification	normal
		History	Given directly from Dr. Jacobs
		Lifespan	finite
		Crisis PDL	48< PDL
		Morphology	fibroblast-like
		Cellosaurus(Expasy)	CVCL_0440
			deposit info	lot info
		Medium	Medium List
		Culture type		Adherent cells
		Culture medium		HF-RITC80-7 (or MEM ALPHA) + 10% FBS
		Antibiotics		Free
		Passage method		0.25% Trypsin
	Culture information	Passage ratio		1 : 4 split
		SC frequency		Subculture : 2 times/week
		Temperature		37 ℃
		CO2 concentration		5 %
		Freeze medium		Medium + 10% DMSO
		Freezing method		Slow freezing
		Mycoplasma/Acholeplasma		(-)
		Virus (HIV)		(-)
		Isozyme		LD, NP
		Chromosome mode		42-47(50) : 42(3),43(3),44(6),45(12),46(24),47(2)
		STR(human)		OK
Images			deposit info	lot info
Reference information		Reference	3
		User's Publication	53

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Reference

167  Jacobs JP.  The status of human diploid cell strain MRC-5 as an approved substrate for the production of viral vaccines  J Biol Stand  1976  4(2):97-9  PubMed ID: 932048   DOI: 10.1016/0092-1157(76)90018-4

168  Jacobs JP.  Updated results on the karyology of the WI-38, MRC-5 and MRC-9 cell strains  Dev Biol Stand  1976  37:155-6  PubMed ID: 1031681

1028  Jacobs, J P, Jones, C M, Baille, J P  Characteristics of a human diploid cell designated MRC-5.  Nature  1970  227:168-70  PubMed ID: 4316953   DOI: 10.1038/227168a0

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User's Publication

19838  Naoi H, Suzuki Y, Miyagi A, Horiguchi R, Aono Y, Inoue Y, Yasui H, Hozumi H, Karayama M, Furuhashi K, Enomoto N, Fujisawa T, Inui N, Mii S, Ichihara M, Takahashi M, Suda T.  CD109 Attenuates Bleomycin-induced Pulmonary Fibrosis by Inhibiting TGF-β Signaling  J Immunol  2024  Feb 9:ji2300285  PubMed ID: 38334455   DOI: 10.4049/jimmunol.2300285

21542  Morihiro K, Morita S, Harada N, Baba M, Yum J, Naito M, Miyata K, Nagae G, Okamoto A.  RNA Oncological Therapeutics: Intracellular Hairpin RNA Assembly Enables MicroRNA-Triggered Anticancer Functionality.  J Am Chem Soc  2024    PubMed ID: 38170469   DOI: 10.1021/jacs.3c09524

21615  Shimura T, Sunaga K, Yamazaki M, Honoka N, Sasatani M, Kamiya K, Ushiyama A.  Nuclear DNA damage-triggered ATM-dependent AMPK activation regulates the mitochondrial radiation response.  Int J Radiat Biol  2024  1-11  PubMed ID: 38166485   DOI: 10.1080/09553002.2023.2295297

21980  Wajima T, Sugawara T, Tanaka E, Uchiya K-i.  Molecular characterization of a novel putative pathogen, Streptococcus nakanoensis sp. nov., isolated from sputum culture.  Microbiol Spectr  2024    PubMed ID: 39269180   DOI: 10.1128/spectrum.01354-24

20512  Shimura T, Nakashiro C, Fujiwara K, Shiga R, Sasatani M, Kamiya K, Ushiyama A.  Radiation affects glutathione redox reaction by reduced glutathione peroxidase activity in human fibroblasts.  J Radiat Res  2022    PubMed ID: 34977941   DOI: 10.1093/jrr/rrab122

20585  Yanagimura N, Takeuchi S, Fukuda K, Arai S, Tanimoto A, Nishiyama A, Ogo N, Takahashi H, Asai A, Watanabe S, Kikuchi T, Yano S.  STAT3 inhibition suppresses adaptive survival of ALK-rearranged lung cancer cells through transcriptional modulation of apoptosis.  NPJ Precis Oncol  2022  6(1):11  PubMed ID: 35228642   DOI: 10.1038/s41698-022-00254-y

16391  Sakamoto T, Yu Z, Otani Y.  Dual-Color Fluorescence Switch-On Probe for Imaging G-Quadruplex and Double-Stranded DNA in Living Cells  Anal Chem  2022  Mar 2  PubMed ID: 35234461   DOI: 10.1021/acs.analchem.1c04804

20770  Ito K, Nishida Y, Hamada S, Shimizu K, Sakai T, Ohkawara B, Alman BA, Enomoto A, Ikuta K, Koike H, Zhang J, Ohno K, Imagama S.  Efficacy of auranofin as an inhibitor of desmoid progression.  Sci Rep  2022  12(1):11918  PubMed ID: 35831372   DOI: 10.1038/s41598-022-15756-9

20785  Ju N, Hayashi H, Shimamura M, Baba S, Yoshida S, Morishita R, Rakugi H, Nakagami H.  Prevention of bleomycin-induced pulmonary fibrosis by a RANKL peptide in mice.  Sci Rep  2022  12(1):12474  PubMed ID: 35864207   DOI: 10.1038/s41598-022-16843-7

20843  Shimura T, Zaharieva E, Sasatani M, Kawai H, Kamiya K, Ushiyama A.  ACTIVATION OF HUMAN FIBROBLASTS BY CHRONIC RADIATION RATHER THAN ACUTE RADIATION.  Radiat Prot Dosimetry  2022  198(13-15):1098-1103  PubMed ID: 36083750   DOI: 10.1093/rpd/ncac065

20934  Shimura T, Totani R, Ogasawara H, Inomata K, Sasatani M, Kamiya K, Ushiyama A.  Effects of oxygen on the response of mitochondria to X-irradiation and reactive oxygen species-mediated fibroblast activation.  Int J Radiat Biol  2022  1-10  PubMed ID: 36383181   DOI: 10.1080/09553002.2023.2142980

17329  Yap JMG, Ueda T, Kanemitsu Y, Takeda N, Fukumitsu K, Fukuda S, Uemura T, Tajiri T, Ohkubo H, Maeno K, Ito Y, Oguri T, Ugawa S, Niimi A.  Human Lung Fibroblasts Exhibit Induced Inflammation Memory via Increased IL6 Gene Expression and Release  Front Immunol  2022  13:921728  PubMed ID: 35941890   DOI: 10.3389/fimmu.2022.921728

20050  Yap JMG, Ueda T, Kanemitsu Y, Takeda N, Fukumitsu K, Fukuda S, Uemura T, Tajiri T, Ohkubo H, Maeno K, Ito Y, Oguri T, Ugawa S, Niimi A.  AITC inhibits fibroblast-myofibroblast transition via TRPA1-independent MAPK and NRF2/HO-1 pathways and reverses corticosteroids insensitivity in human lung fibroblasts.  Respir Res  2021  22(1):51  PubMed ID: 33579280   DOI: 10.1186/s12931-021-01636-9

20392  Shikata M, Toyooka T, Komaki Y, Ibuki Y.  4-(Methylnitrosamino)-1-(3-pyridyl)-1-butanone-Induced Histone Acetylation via α7nAChR-Mediated PI3K/Akt Activation and Its Impact on γ-H2AX Generation.  Chem Res Toxicol  2021    PubMed ID: 34784199   DOI: 10.1021/acs.chemrestox.1c00287

9839  Tomohito Yoshihara, Yasuhiro Nanri, Satoshi Nunomura, Yukie Yamaguchi, Carol Feghali-Bostwick, Keiichi Ajito, Shoichi Murakami 4, Masaaki Mawatari, Kenji Izuhara  Periostin Plays a Critical Role in the Cell Cycle in Lung Fibroblasts  Respir Res  2020  21(1):38  PubMed ID: 32000779   DOI: 10.1186/s12931-020-1299-0

12045  Yap JMG, Ueda T, Takeda N, Fukumitsu K, Fukuda S, Uemura T, Tajiri T, Ohkubo H, Maeno K, Ito Y, Kanemitsu Y, Niimi A.  An inflammatory stimulus sensitizes TRPA1 channel to increase cytokine release in human lung fibroblasts.  Cytokine  2020    PubMed ID: 32050145   DOI: 10.1016/j.cyto.2020.155027

13016  Sano M, Yada R, Nomura Y, Kusukawa T, Ando H, Matsumoto K, Wada K, Tanaka T, Ohara H, Aso Y.  Microbial Screening Based on the Mizoroki-Heck Reaction Permits Exploration of Hydroxyhexylitaconic-Acid-Producing Fungi in Soils.  Microorganisms  2020    PubMed ID: 32365722   DOI: 10.3390/microorganisms8050648

13087  Nakano T, Ohara Y, Fujita H, Ainai A, Yamamura ET, Suzuki T, Hasegawa H, Sone T, Asano K.  Double-Stranded Structure of the Polyinosinic-Polycytidylic Acid Molecule to Elicit TLR3 Signaling and Adjuvant Activity in Murine Intranasal A(H1N1)pdm09 Influenza Vaccination.  DNA Cell Biol  2020    PubMed ID: 32580635   DOI: 10.1089/dna.2019.5324

13278  Kometani T, Arai T, Chibazakura T.  Increased Expression of NPM1 Suppresses p27Kip1 Function in Cancer Cells.  Cancers (Basel)  2020    PubMed ID: 33050036   DOI: 10.3390/cancers12102886

13531  Yamamoto Y, Korogi Y, Hirai T, Gotoh S.  A method of generating alveolar organoids using human pluripotent stem cells  Methods Cell Biol  2020  159:115-14  PubMed ID: 32586440   DOI: 10.1016/bs.mcb.2020.02.004

11423  Fukuda K, Takeuchi S, Arai S, Katayama R, Nanjo S, Tanimoto A, Nishiyama A, Nakagawa T, Taniguchi H, Suzuki T, Yamada T, Nishihara H, Ninomiya H, Ishikawa Y, Baba S, Takeuchi K, Horiike A, Yanagitani N, Nishio M, Yano S.  Epithelial-to-Mesenchymal Transition Is a Mechanism of ALK Inhibitor Resistance in Lung Cancer Independent of ALK Mutation Status.  Cancer Res.  2019    PubMed ID: 30737231   DOI: 10.1158/0008-5472.CAN-18-2052

11587  Shiraishi K, Nakajima T, Shichino S, Deshimaru S, Matsushima K, Ueha S.  In vitro expansion of endogenous human alveolar epithelial type II cells in fibroblast-free spheroid culture.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31178143   DOI: 10.1016/j.bbrc.2019.05.187

6901  Terasaki M, Ikuta M, Kojima H, Tanaka T, Maeda H, Miyashita K, Mutoh M.  Dietary Fucoxanthin Induces Anoikis in Colorectal Adenocarcinoma by Suppressing Integrin Signaling in a Murine Colorectal Cancer Model.  J Clin Med  2019  9(1):90  PubMed ID: 31905803   DOI: 10.3390/jcm9010090

10822  Takashina T, Koyama T, Nohara S, Hasegawa M, Ishiguro A, Iijima K, Lu J, Shimura M, Okamura T, Sakuma T, Yamamoto T, Ishizaka Y.  Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering.  Biomaterials  2018  173:11-21  PubMed ID: 29734017   DOI: 10.1016/j.biomaterials.2018.04.040

11194  Park AM, Tsunoda I, Yoshie O.  Heat shock protein 27 promotes cell cycle progression by down-regulating E2F transcription factor 4 and retinoblastoma family protein p130.  J. Biol. Chem.  2018  293(41):15815-15826  PubMed ID: 30166342   DOI: 10.1074/jbc.RA118.003310

4305  Nakano T, Yamamura ET, Fujita H, Sone T, Asano K.  Novel methods for nucleotide length control in double-stranded polyinosinic-polycytidylic acid production using uneven length components.  Biosci. Biotechnol. Biochem.  2018    PubMed ID: 30079840   DOI: 10.1080/09168451.2018.1501264

7019  Yamamoto Y, Gotoh S, Korogi Y, Seki M, Konishi S, Ikeo S, Sone N, Nagasaki T, Matsumoto H, Muro S, Ito I, Hirai T, Kohno T, Suzuki Y, Mishima M.  Long-term expansion of alveolar stem cells derived from human iPS cells in organoids.  Nat. Methods  2017  14:1097-1106  PubMed ID: 28967890   DOI: 10.1038/nmeth.4448

10135  Komiya K, Ohta S, Arima K, Ogawa M, Suzuki S, Mitamura Y, Nunomura S, Nanri Y, Yoshihara T, Kawaguchi A, Kadota JI, Rubin BK, Izuhara K.  Clarithromycin attenuates IL-13-induced periostin production in human lung fibroblasts.  Respir. Res.  2017  18:37  PubMed ID: 28219384   DOI: 10.1186/s12931-017-0519-8

10487  Adachi Y, Watanabe K, Kita K, Kitai H, Kotani H, Sato Y, Inase N, Yano S, Ebi H.  Resistance mediated by alternative receptor tyrosine kinases in FGFR1-amplified lung cancer.  Carcinogenesis  2017  38:1063-1072  PubMed ID: 28968756   DOI: 10.1093/carcin/bgx091

5446  Endo S1, Nakata K2, Ohuchida K3, Takesue S1, Nakayama H1, Abe T1, Koikawa K, Okumura T, Sada M, Horioka K, Zheng B, Mizuuchi Y, Iwamoto C5, Murata M, Moriyama T, Miyasaka Y, Ohtsuka T, Mizumoto K, Oda Y, Hashizume M, Nakamura M  Autophagy Is Required for Activation of Pancreatic Stellate Cells, Associated With Pancreatic Cancer Progression and Promotes Growth of Pancreatic Tumors in Mice.  Gastroenterology  2017  152(6):1492-1506  PubMed ID: 28126348   DOI: 10.1053/j.gastro.2017.01.010

14607  Nanjo S, Arai S, Wang W, Takeuchi S, Yamada T, Hata A, Katakami N, Okada Y, Yano S.  MET Copy Number Gain Is Associated with Gefitinib Resistance in Leptomeningeal Carcinomatosis of EGFR-mutant Lung Cancer  Mol Cancer Ther  2017  16(3):506-515  PubMed ID: 28138027   DOI: 10.1158/1535-7163.MCT-16-0522

12930  Park AM, Kanai K, Itoh T, Sato T, Tsukui T, Inagaki Y, Selman M, Matsushima K, Yoshie O.  Myofibroblast Differentiation and in the Development of Bleomycin-Induced Pulmonary Fibrosis  PLoS One  2016  11(2):e0148998  PubMed ID: 26859835   DOI: 10.1371/journal.pone.0148998

10168  Taku Chibazakura, Yui Toriyabe, Hiroshi Fujii, Kiwamu Takahashi, Mariko Kawakami, Haruna Kuwamura, Hazuki Haga, Shun-ichiro Ogura, Fuminori Abe, Motowo Nakajima, Hirofumi Yoshikawa, Tohru Tanaka  5-Aminolevulinic Acid Enhances Cell Death Under Thermal Stress in Certain Cancer Cell Lines  Biosci Biotechnol Biochem   2015  79(3):422-31  PubMed ID: 25346276   DOI: 10.1080/09168451.2014.975186

4396  Yamada T, Takeuchi S, Kita K, Bando H, Nakamura T, Matsumoto K, Yano S.  Hepatocyte growth factor induces resistance to anti-epidermal growth factor receptor antibody in lung cancer.  J Thorac Oncol  2012  7(2):272-80  PubMed ID: 22089117   DOI: 10.1097/JTO.0b013e3182398e69

7848  Yasui T, Ohuchida K, Zhao M, Cui L, Onimaru M, Egami T, Fujita H, Ohtsuka T, Mizumoto K, Matsumoto K, Tanaka M.  Adenoviral therapy is more effective in gemcitabine-resistant pancreatic cancer than in gemcitabine-sensitive cells.  Anticancer Res.  2011  31:1279-87  PubMed ID: 21508376

10407  Hayakawa S, Shiratori S, Yamato H, Kameyama T, Kitatsuji C, Kashigi F, Goto S, Kameoka S, Fujikura D, Yamada T, Mizutani T, Kazumata M, Sato M, Tanaka J, Asaka M, Ohba Y, Miyazaki T, Imamura M, Takaoka A.  ZAPS is a potent stimulator of signaling mediated by the RNA helicase RIG-I during antiviral responses.  Nat. Immunol.  2011  12:37-44  PubMed ID: 21102435   DOI: 10.1038/ni.1963

8639  Hirata N, Takahashi M, Yazawa M.  Diphosphorylation of regulatory light chain of myosin IIA is responsible for proper cell spreading.  Biochem. Biophys. Res. Commun.  2009  381:682-7  PubMed ID: 19254691   DOI: 10.1016/j.bbrc.2009.02.121

8835  Yoshida K, Munakata H.  Connective tissue growth factor binds to fibronectin through the type I repeat modules and enhances the affinity of fibronectin to fibrin.  Biochim. Biophys. Acta  2007  1770:672-80  PubMed ID: 17239539   DOI: 10.1016/j.bbagen.2006.11.010

3434  Nikawa H, Egusa H, Makihira S, Okamoto T, Kurihara H, Shiba H, Amano H, Murayama T, Yatani H, Hamada T.  An in vitro evaluation of the adhesion of Candida species to oral and lung tissue cells.  Mycoses  2006  49:14-7  PubMed ID: 16367812   DOI: 10.1111/j.1439-0507.2005.01176.x

3413  Yamashita, Satoko, Katayama, Hideki, Saitoh, Hiroyuki, Akao, Tetsuyuki, Park, Yu Shin, Mizuki, Eiichi, Ohba, Michio, Ito, Akio  Typical Three-Domain Cry Proteins of Bacillus thuringiensis Strain A1462 Exhibit Cytocidal Activity on Limited Human Cancer Cells.  J Biochem  2005  138:663-72  PubMed ID: 16428294   DOI: 10.1093/jb/mvi177

2892  Katayama H, Yokota H, Akao T, Nakamura O, Ohba M, Mekada E, Mizuki E  Parasporin-1, a novel cytotoxic protein to human cells from non-insecticidal parasporal inclusions of Bacillus thuringiensis  J Biochem (Tokyo)  2005  137:17-25  PubMed ID: 15713879   DOI: 10.1093/jb/mvi003

3202  Ohuchida, Kenoki, Mizumoto, Kazuhiro, Ogura, Yasuhiro, Ishikawa, Nami, Nagai, Eishi, Yamaguchi, Koji, Tanaka, Masao  Quantitative assessment of telomerase activity and human telomerase reverse transcriptase messenger RNA levels in pancreatic juice samples for the diagnosis of pancreatic cancer.  Clin Cancer Res  2005  11:2285-92  PubMed ID: 15788678   DOI: 10.1158/1078-0432.CCR-04-1581

2894  Ito, Akio, Sasaguri, Yasuyuki, Kitada, Sakae, Kusaka, Yoshitomo, Kuwano, Kyoko, Masutomi, Kenjiro, Mizuki, Eiichi, Akao, Tetsuyuki, Ohba, Michio  A Bacillus thuringiensis crystal protein with selective cytocidal action to human cells.  J Biol Chem  2004  279:21282-6  PubMed ID: 15026424   DOI: 10.1074/jbc.M401881200

2672  Manabe, Tatsuya, Mizumoto, Kazuhiro, Nagai, Eishi, Matsumoto, Kunio, Nakamura, Toshikazu, Nukiwa, Toshihiro, Tanaka, Masao, Matsuda, Takehisa  Cell-based protein delivery system for the inhibition of the growth of pancreatic cancer: NK4 gene-transduced oral mucosal epithelial cell sheet.  Clin Cancer Res  2003  9:3158-66  PubMed ID: 12912968

2709  Yagi, Yasuyuki, Sotani, Tomohiro, Nagao, Toshinori, Horio, Tomoyo, Yamamoto, Itaru, Gohda, Eiichi  Induction by staurosporine of hepatocyte growth factor production in human skin fibroblasts independent of protein kinase inhibition.  Biochem Pharmacol  2003  66:1797-808  PubMed ID: 14563490   DOI: 10.1016/s0006-2952(03)00547-1

9427  Yoshida K, Suzuki Y, Honda E, Amemiya K, Nakatani T, Ebina M, Narumi K, Satoh K, Munakata H.  Leucine-rich repeat region of decorin binds to filamin-A.  Biochimie  2002  84:303-8  PubMed ID: 12106908   DOI: 10.1016/s0300-9084(02)01391-3

3786  Ogawa-Goto K, Irie S, Omori A, Miura Y, Katano H, Hasegawa H, Kurata T, Sata T, Arao Y.  An endoplasmic reticulum protein, p180, is highly expressed in human cytomegalovirus-permissive cells and interacts with the tegument protein encoded by UL48.  J Virol  2002  76(5):2350-62  PubMed ID: 11836413   DOI: 10.1128/jvi.76.5.2350-2362.2002

2631  Saitoh T, Takemura S, Ueda K, Hosoya H, Nagayama M, Haga H, Kawabata K, Yamagishi A, Takahashi M  Differential localization of non-muscle myosin II isoforms and phosphorylated regulatory light chains in human MRC-5 fibroblasts.  FEBS Lett  2001  509:365-9  PubMed ID: 11749957   DOI: 10.1016/s0014-5793(01)03186-6

2531  Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG.  Short tandem repeat profiling provides an international reference standard for human cell lines.  Proc Natl Acad Sci U S A.  2001  98(14):8012-7  PubMed ID: 11416159   DOI: 10.1073/pnas.121616198

2070  Shindoh M, Higashino F, Kaya M, Yasuda M, Funaoka K, Hanzawa M, Hida K, Kohgo T, Amemiya A, Yoshida K, Fujinaga K  Correlated expression of matrix metalloproteinases and ets family transcription factor E1A-F in invasive oral squamous-cell-carcinoma-derived cell lines.  Am J Pathol  1996  148:693-700  PubMed ID: 8774124

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10096  Zhao FQ, Glimm DR, Kennelly JJ.  Distribution of mammalian facilitative glucose transporter messenger RNA in bovine tissues.  Int. J. Biochem.  1993  25:1897-903  PubMed ID: 8138027   DOI: 10.1016/0020-711x(88)90322-9

1809  Gohda E, Matsunaga T, Kataoka H, Yamamoto I  TGF-beta is a potent inhibitor of hepatocyte growth factor secretion by human fibroblasts.  Cell Biol Int Rep  1992  16:917-26  PubMed ID: 1423659   DOI: 10.1016/s0309-1651(06)80171-2