Search
CELL BANK Website

Back Back
Cell No. : Cell Name
RCB0218 : MRC-5, known PDL  update : 2018/07/06
CommentNormal embryonic lung fibroblast with accurate PDL
Comment from the depositor
Terms and conditionsBasically, there is no restriction regarding academic use.
Remarks
Order Form Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, Category I and II, depending on the sort of user institutions and the purposes of use.Please use an appropriate MTA(to see).In case of Restriction "a" or "f", please contact RIKEN BRC(cellbank.brc@riken.jp) regarding any kind of for-profit use.
Basic information Depositor Ohno, Tadao
Originator Jacobs, J. P.
Year of deposit 1987
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 14 weeks embryo
Tissue embryo/fetus, lung
Classification normal
History Given directly from Dr. Jacobs
Lifespan finite
Crisis PDL 48< PDL
Morphology fibroblast-like
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HF-RITC80-7 (or MEM ALPHA) + 10% FBS
Antibiotics Free
Passage method 0.25% trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
STR(human) OK
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 42-47(50) : 42(3),43(3),44(6),45(12),46(24),47(2)
Images
deposit info
lot info
Reference information Reference 1
User's Publication 34


To topTop
Reference
1028  Jacobs, J P, Jones, C M, Baille, J P  Characteristics of a human diploid cell designated MRC-5.  Nature  1970  227:168-70  PubMed ID: 4316953

To topTop
User's Publication
9839  Tomohito Yoshihara, Yasuhiro Nanri, Satoshi Nunomura, Yukie Yamaguchi, Carol Feghali-Bostwick, Keiichi Ajito, Shoichi Murakami 4, Masaaki Mawatari, Kenji Izuhara  Periostin Plays a Critical Role in the Cell Cycle in Lung Fibroblasts  Respir Res  2020  21(1):38  PubMed ID: 32000779
12045  Yap JMG, Ueda T, Takeda N, Fukumitsu K, Fukuda S, Uemura T, Tajiri T, Ohkubo H, Maeno K, Ito Y, Kanemitsu Y, Niimi A.  An inflammatory stimulus sensitizes TRPA1 channel to increase cytokine release in human lung fibroblasts.  Cytokine  2020    PubMed ID: 32050145
11423  Fukuda K, Takeuchi S, Arai S, Katayama R, Nanjo S, Tanimoto A, Nishiyama A, Nakagawa T, Taniguchi H, Suzuki T, Yamada T, Nishihara H, Ninomiya H, Ishikawa Y, Baba S, Takeuchi K, Horiike A, Yanagitani N, Nishio M, Yano S.  Epithelial-to-Mesenchymal Transition Is a Mechanism of ALK Inhibitor Resistance in Lung Cancer Independent of ALK Mutation Status.  Cancer Res.  2019    PubMed ID: 30737231
11587  Shiraishi K, Nakajima T, Shichino S, Deshimaru S, Matsushima K, Ueha S.  In vitro expansion of endogenous human alveolar epithelial type II cells in fibroblast-free spheroid culture.  Biochem. Biophys. Res. Commun.  2019    PubMed ID: 31178143
6901  Terasaki M, Ikuta M, Kojima H, Tanaka T, Maeda H, Miyashita K, Mutoh M.  Dietary Fucoxanthin Induces Anoikis in Colorectal Adenocarcinoma by Suppressing Integrin Signaling in a Murine Colorectal Cancer Model.  J Clin Med  2019  9(1):90  PubMed ID: 31905803
10822  Takashina T, Koyama T, Nohara S, Hasegawa M, Ishiguro A, Iijima K, Lu J, Shimura M, Okamura T, Sakuma T, Yamamoto T, Ishizaka Y.  Identification of a cell-penetrating peptide applicable to a protein-based transcription activator-like effector expression system for cell engineering.  Biomaterials  2018  173:11-21  PubMed ID: 29734017
11194  Park AM, Tsunoda I, Yoshie O.  Heat shock protein 27 promotes cell cycle progression by down-regulating E2F transcription factor 4 and retinoblastoma family protein p130.  J. Biol. Chem.  2018  293(41):15815-15826  PubMed ID: 30166342
4305  Nakano T, Yamamura ET, Fujita H, Sone T, Asano K.  Novel methods for nucleotide length control in double-stranded polyinosinic-polycytidylic acid production using uneven length components.  Biosci. Biotechnol. Biochem.  2018    PubMed ID: 30079840
7019  Yamamoto Y, Gotoh S, Korogi Y, Seki M, Konishi S, Ikeo S, Sone N, Nagasaki T, Matsumoto H, Muro S, Ito I, Hirai T, Kohno T, Suzuki Y, Mishima M.  Long-term expansion of alveolar stem cells derived from human iPS cells in organoids.  Nat. Methods  2017  14:1097-1106  PubMed ID: 28967890
10135  Komiya K, Ohta S, Arima K, Ogawa M, Suzuki S, Mitamura Y, Nunomura S, Nanri Y, Yoshihara T, Kawaguchi A, Kadota JI, Rubin BK, Izuhara K.  Clarithromycin attenuates IL-13-induced periostin production in human lung fibroblasts.  Respir. Res.  2017  18:37  PubMed ID: 28219384
10487  Adachi Y, Watanabe K, Kita K, Kitai H, Kotani H, Sato Y, Inase N, Yano S, Ebi H.  Resistance mediated by alternative receptor tyrosine kinases in FGFR1-amplified lung cancer.  Carcinogenesis  2017  38:1063-1072  PubMed ID: 28968756
5446  Endo S1, Nakata K2, Ohuchida K3, Takesue S1, Nakayama H1, Abe T1, Koikawa K, Okumura T, Sada M, Horioka K, Zheng B, Mizuuchi Y, Iwamoto C5, Murata M, Moriyama T, Miyasaka Y, Ohtsuka T, Mizumoto K, Oda Y, Hashizume M, Nakamura M  Autophagy Is Required for Activation of Pancreatic Stellate Cells, Associated With Pancreatic Cancer Progression and Promotes Growth of Pancreatic Tumors in Mice.  Gastroenterology  2017  152(6):1492-1506  PubMed ID: 28126348
10168  Taku Chibazakura, Yui Toriyabe, Hiroshi Fujii, Kiwamu Takahashi, Mariko Kawakami, Haruna Kuwamura, Hazuki Haga, Shun-ichiro Ogura, Fuminori Abe, Motowo Nakajima, Hirofumi Yoshikawa, Tohru Tanaka  5-Aminolevulinic Acid Enhances Cell Death Under Thermal Stress in Certain Cancer Cell Lines  Biosci Biotechnol Biochem   2015  79(3):422-31  PubMed ID: 25346276
4396  Yamada T, Takeuchi S, Kita K, Bando H, Nakamura T, Matsumoto K, Yano S.  Hepatocyte growth factor induces resistance to anti-epidermal growth factor receptor antibody in lung cancer.  J Thorac Oncol  2012  7(2):272-80  PubMed ID: 22089117
7848  Yasui T, Ohuchida K, Zhao M, Cui L, Onimaru M, Egami T, Fujita H, Ohtsuka T, Mizumoto K, Matsumoto K, Tanaka M.  Adenoviral therapy is more effective in gemcitabine-resistant pancreatic cancer than in gemcitabine-sensitive cells.  Anticancer Res.  2011  31:1279-87  PubMed ID: 21508376
10407  Hayakawa S, Shiratori S, Yamato H, Kameyama T, Kitatsuji C, Kashigi F, Goto S, Kameoka S, Fujikura D, Yamada T, Mizutani T, Kazumata M, Sato M, Tanaka J, Asaka M, Ohba Y, Miyazaki T, Imamura M, Takaoka A.  ZAPS is a potent stimulator of signaling mediated by the RNA helicase RIG-I during antiviral responses.  Nat. Immunol.  2011  12:37-44  PubMed ID: 21102435
8639  Hirata N, Takahashi M, Yazawa M.  Diphosphorylation of regulatory light chain of myosin IIA is responsible for proper cell spreading.  Biochem. Biophys. Res. Commun.  2009  381:682-7  PubMed ID: 19254691
8835  Yoshida K, Munakata H.  Connective tissue growth factor binds to fibronectin through the type I repeat modules and enhances the affinity of fibronectin to fibrin.  Biochim. Biophys. Acta  2007  1770:672-80  PubMed ID: 17239539
3413  Yamashita, Satoko, Katayama, Hideki, Saitoh, Hiroyuki, Akao, Tetsuyuki, Park, Yu Shin, Mizuki, Eiichi, Ohba, Michio, Ito, Akio  Typical Three-Domain Cry Proteins of Bacillus thuringiensis Strain A1462 Exhibit Cytocidal Activity on Limited Human Cancer Cells.  J Biochem  2005  138:663-72  PubMed ID: 16428294
2892  Katayama H, Yokota H, Akao T, Nakamura O, Ohba M, Mekada E, Mizuki E  Parasporin-1, a novel cytotoxic protein to human cells from non-insecticidal parasporal inclusions of Bacillus thuringiensis  J Biochem (Tokyo)  2005  137:17-25  PubMed ID: 15713879
3202  Ohuchida, Kenoki, Mizumoto, Kazuhiro, Ogura, Yasuhiro, Ishikawa, Nami, Nagai, Eishi, Yamaguchi, Koji, Tanaka, Masao  Quantitative assessment of telomerase activity and human telomerase reverse transcriptase messenger RNA levels in pancreatic juice samples for the diagnosis of pancreatic cancer.  Clin Cancer Res  2005  11:2285-92  PubMed ID: 15788678
3434  Nikawa H, Egusa H, Makihira S, Okamoto T, Kurihara H, Shiba H, Amano H, Murayama T, Yatani H, Hamada T.  An in vitro evaluation of the adhesion of Candida species to oral and lung tissue cells.  Mycoses  2005  49:14-7  PubMed ID: 16367812
2894  Ito, Akio, Sasaguri, Yasuyuki, Kitada, Sakae, Kusaka, Yoshitomo, Kuwano, Kyoko, Masutomi, Kenjiro, Mizuki, Eiichi, Akao, Tetsuyuki, Ohba, Michio  A Bacillus thuringiensis crystal protein with selective cytocidal action to human cells.  J Biol Chem  2004  279:21282-6  PubMed ID: 15026424
2672  Manabe, Tatsuya, Mizumoto, Kazuhiro, Nagai, Eishi, Matsumoto, Kunio, Nakamura, Toshikazu, Nukiwa, Toshihiro, Tanaka, Masao, Matsuda, Takehisa  Cell-based protein delivery system for the inhibition of the growth of pancreatic cancer: NK4 gene-transduced oral mucosal epithelial cell sheet.  Clin Cancer Res  2003  9:3158-66  PubMed ID: 12912968
2709  Yagi, Yasuyuki, Sotani, Tomohiro, Nagao, Toshinori, Horio, Tomoyo, Yamamoto, Itaru, Gohda, Eiichi  Induction by staurosporine of hepatocyte growth factor production in human skin fibroblasts independent of protein kinase inhibition.  Biochem Pharmacol  2003  66:1797-808  PubMed ID: 14563490
10894  Hiroyuki Oshiumi, Miwa Sasai, Kyoko Shida, Takashi Fujita, Misako Matsumoto, Tsukasa Seya  TIR-containing Adapter Molecule (TICAM)-2, a Bridging Adapter Recruiting to Toll-Like Receptor 4 TICAM-1 That Induces Interferon-Beta  J Biol Chem  2003  278(50):49751-62  PubMed ID: 14519765
9427  Yoshida K, Suzuki Y, Honda E, Amemiya K, Nakatani T, Ebina M, Narumi K, Satoh K, Munakata H.  Leucine-rich repeat region of decorin binds to filamin-A.  Biochimie  2002  84:303-8  PubMed ID: 12106908
3786  Ogawa-Goto K, Irie S, Omori A, Miura Y, Katano H, Hasegawa H, Kurata T, Sata T, Arao Y.  An endoplasmic reticulum protein, p180, is highly expressed in human cytomegalovirus-permissive cells and interacts with the tegument protein encoded by UL48.  J Virol  2002  76(5):2350-62  PubMed ID: 11836413
2631  Saitoh T, Takemura S, Ueda K, Hosoya H, Nagayama M, Haga H, Kawabata K, Yamagishi A, Takahashi M  Differential localization of non-muscle myosin II isoforms and phosphorylated regulatory light chains in human MRC-5 fibroblasts.  FEBS Lett  2001  509:365-9  PubMed ID: 11749957
2531  Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG.  Short tandem repeat profiling provides an international reference standard for human cell lines.  Proc Natl Acad Sci U S A.  2001  98(14):8012-7  PubMed ID: 11416159
2070  Shindoh M, Higashino F, Kaya M, Yasuda M, Funaoka K, Hanzawa M, Hida K, Kohgo T, Amemiya A, Yoshida K, Fujinaga K  Correlated expression of matrix metalloproteinases and ets family transcription factor E1A-F in invasive oral squamous-cell-carcinoma-derived cell lines.  Am J Pathol  1996  148:693-700  PubMed ID: 8774124
1808  Matsunaga T, Gohda E, Takebe T, Wu, Y L, Iwao M, Kataoka H, Yamamoto I  Expression of hepatocyte growth factor is up-regulated through activation of a cAMP-mediated pathway.  Exp Cell Res  1994  210:326-35  PubMed ID: 7507855
10096  Zhao FQ, Glimm DR, Kennelly JJ.  Distribution of mammalian facilitative glucose transporter messenger RNA in bovine tissues.  Int. J. Biochem.  1993  25:1897-903  PubMed ID: 8138027
1809  Gohda E, Matsunaga T, Kataoka H, Yamamoto I  TGF-beta is a potent inhibitor of hepatocyte growth factor secretion by human fibroblasts.  Cell Biol Int Rep  1992  16:917-26  PubMed ID: 1423659



Back Back Return Top Page