Cell No. : Cell Name
RCB0211 : MRC-5
update : 2023/04/12
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| Comment | Distribution terminated. Normal embryonic lung fibroblast. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to the website of ECACC prior to order. |
| Comment from the depositor | |
| Terms and conditions | There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
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Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
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Depositor |
ECACC
|
| Originator |
Jacobs, J. P.
|
| Year of deposit |
1987
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
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| Species |
sapiens
|
| Gender |
Male
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| Age at sampling |
fetus
|
| Tissue |
lung
|
| Classification |
normal
|
| History |
overseas, ECACC
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|
Lifespan |
finite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_0440
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deposit info |
lot info |
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Medium |
Medium List |
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Culture type |
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Adherent cells
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| Culture medium |
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MEM ALPHA (or HF-RITC80-7) + 10% FBS
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| Antibiotics |
|
Free
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|
Passage method |
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0.25% Trypsin
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Culture information
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Passage ratio |
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1 : 4 split
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| SC frequency |
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Subculture : 2 times/week
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| Temperature |
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37
℃
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| CO2 concentration |
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5
%
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| Freeze medium |
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Medium + 10% DMSO
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| Freezing method |
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Slow freezing
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| Mycoplasma/Acholeplasma |
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(-)
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| Virus (HIV) |
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(-)
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| Isozyme |
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LD, NP
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| Chromosome mode |
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44-47(50) : 44(3),45(7),46(39),47(1)
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| STR(human) |
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OK
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| Images |
deposit info | lot info |
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| Reference information |
Reference |
2
|
| User's Publication |
11
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| User's Publication |
|
19992
Nishi M, Tateishi K, Sundararaj JS, Ino Y, Nakai Y, Hatayama Y, Yamaoka Y, Mihana Y, Miyakawa K, Kimura H, Kimura Y, Yamamoto T, Ryo A.
Development of a contacting transwell co-culture system for the in vitro propagation of primary central nervous system lymphoma
Front Cell Dev Biol
2023
Nov 27:11:1275519
PubMed ID: 38089883
DOI: 10.3389/fcell.2023.1275519
|
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14787
Yamaoka Y, Miyakawa K, Jeremiah SS, Funabashi R, Okudela K, Kikuchi S, Katada J, Wada A, Takei T, Nishi M, Shimizu K, Ozawa H, Usuku S, Kawakami C, Tanaka N, Morita T, Hayashi H, Mitsui H, Suzuki K, Aizawa D, Yoshimura Y, Miyazaki T, Yamazaki E, Suzuki T, Kimura H, Shimizu H, Okabe N, Hasegawa H, Ryo A.
Highly specific monoclonal antibodies and epitope identification against SARS-CoV-2 nucleocapsid protein for antigen detection tests
Cell Rep Med
2021
2(6):100311
PubMed ID: 34027498
DOI: 10.1016/j.xcrm.2021.100311
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4075
Kawasaki Y, Tsuboi C, Yagi K, Morizane M, Masaoka Y, Esumi S, Kitamura Y, Sendo T.
Photoinitiators enhanced 1,2-dichloropropane-induced cytotoxicity in human normal embryonic lung fibroblasts cells in vitro
Environ Sci Pollut Res Int
2015
22(6):4763-70
PubMed ID: 25501643
DOI: 10.1007/s11356-014-3939-8
|
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5830
Hirai S, Endo S, Saito R, Hirose M, Ueno T, Suzuki H, Yamato K, Abei M, Hyodo I.
Antitumor effects of a sirtuin inhibitor, tenovin-6, against gastric cancer cells via death receptor 5 up-regulation.
PLoS One
2014
9(7):e102831
PubMed ID: 25033286
DOI: 10.1371/journal.pone.0102831
|
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10293
Takunori Ueno, Shinji Endo, Rie Saito, Mitsuaki Hirose, Sachiko Hirai, Hideo Suzuki, Kenji Yamato, Ichinosuke Hyodo
The Sirtuin Inhibitor Tenovin-6 Upregulates Death Receptor 5 and Enhances Cytotoxic Effects of 5-Fluorouracil and Oxaliplatin in Colon Cancer Cells
Oncol Res
2013
21(3):155-64
PubMed ID: 24512730
DOI: 10.3727/096504013X13854886566598
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15922
Fujitani N, Furukawa J, Araki K, Fujioka T, Takegawa Y, Piao J, Nishioka T, Tamura T, Nikaido T, Ito M, Nakamura Y, Shinohara Y.
Total cellular glycomics allows characterizing cells and streamlining the discovery process for cellular biomarkers
Proc Natl Acad Sci U S A
2013
110(6):2105-10
PubMed ID: 23345451
DOI: 10.1073/pnas.1214233110
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18936
Wu D, Matsushita K, Matsubara H, Nomura F, Tomonaga T.
An alternative splicing isoform of eukaryotic initiation factor 4H promotes tumorigenesis in vivo and is a potential therapeutic target for human cancer
Int J Cancer
2011
128(5):1018-30
PubMed ID: 20473909
DOI: 10.1002/ijc.25419
|
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19223
Andersen JB, Spee B, Blechacz BR, Avital I, Komuta M, Barbour A, Conner EA, Gillen MC, Roskams T, Roberts LR, Factor VM, Thorgeirsson SS.
alpha-Smooth Muscle Actin Expressing Stroma Promotes an Aggressive Tumor Biology in Pancreatic Ductal Adenocarcinoma
Pancreas
2010
39(8):1254-1262
PubMed ID: 20467342
DOI: 10.1097/MPA.0b013e3181dbf647
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19309
Nakatani T, Honda E, Hayakawa S, Sato M, Satoh K, Kudo M, Munakata H.
Effects of decorin on the expression of alpha-smooth muscle actin in a human myofibroblast cell line
Mol Cell Biochem
2008
308(1-2):201-7
PubMed ID: 17952560
DOI: 10.1007/s11010-007-9629-9
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10360
Masaaki K Sato, Masayuki Takahashi, Michio Yazawa
Two Regions of the Tail Are Necessary for the Isoform-Specific Functions of Nonmuscle Myosin IIB
Mol Biol Cell
2007
18(3):1009-17
PubMed ID: 17202408
DOI: 10.1091/mbc.e06-08-0706
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2304
Ohuchida K, Mizumoto K, Murakami M, Qian LW, Sato N, Nagai E, Matsumoto K, Nakamura T, Tanaka M.
Radiation to stromal fibroblasts increases invasiveness of pancreatic cancer cells through tumor-stromal interactions
Cancer Res
2004
64(9):3215-22.
PubMed ID: 15126362
DOI: 10.1158/0008-5472.can-03-2464
|
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