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Cell No. : Cell Name
RCB0210 : HF19  update : 2022/03/08
CommentNormal human lung fibroblast. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA 85011418 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85011418 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ECACC
Year of deposit 1987
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling fetus
Tissue lung
Classification normal
History overseas, ECACC
Lifespan finite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_2487
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HF-RITC80-7 (or MEM ALPHA) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 4 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 45-92(50) : 45(4),46(44),89(1),92(1)
STR(human) OK
Images
deposit info
lot info
Reference information Reference 2
User's Publication 9


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Reference
4663  Cox R, Thacker J, Goodhead DT.  Inactivation and mutation of cultured mammalian cells by aluminium characteristic ultrasoft X-rays. II. Dose-responses of Chinese hamster and human diploid cells to aluminium X-rays and radiations of different LET.  Int J Radiat Biol Relat Stud Phys Chem Med  1977  31(6):561-76  PubMed ID: 301865   DOI: 10.1080/09553007714550661
4664  Goodhead DT.  Inactivation and mutation of cultured mammalian cells by aluminium characteristic ultrasoft X-rays. III. Implication for theory of dual radiation action.  Int J Radiat Biol Relat Stud Phys Chem Med  1977  32(1):43-70  PubMed ID: 301870   DOI: 10.1080/09553007714550751

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User's Publication
20050  Yap JMG, Ueda T, Kanemitsu Y, Takeda N, Fukumitsu K, Fukuda S, Uemura T, Tajiri T, Ohkubo H, Maeno K, Ito Y, Oguri T, Ugawa S, Niimi A.  AITC inhibits fibroblast-myofibroblast transition via TRPA1-independent MAPK and NRF2/HO-1 pathways and reverses corticosteroids insensitivity in human lung fibroblasts.  Respir Res  2021  22(1):51  PubMed ID: 33579280   DOI: 10.1186/s12931-021-01636-9
12045  Yap JMG, Ueda T, Takeda N, Fukumitsu K, Fukuda S, Uemura T, Tajiri T, Ohkubo H, Maeno K, Ito Y, Kanemitsu Y, Niimi A.  An inflammatory stimulus sensitizes TRPA1 channel to increase cytokine release in human lung fibroblasts.  Cytokine  2020    PubMed ID: 32050145   DOI: 10.1016/j.cyto.2020.155027
12150  Amira Mira, Mohamed A. Sabry, Kuniyoshi Shimizu & Fatma M. Abdel Bar  A new pimarane-type diterpene obtained by biotransformation inhibits human HCT-116 colorectal carcinoma through inhibition of LTA4H activity  Original Research  2020      DOI: 10.1007/s00044-020-02520-9
5680  Ginting TE, Suryatenggara J, Christian S, Mathew G.  Proinflammatory response induced by Newcastle disease virus in tumor and normal cells.  Oncolytic Virother  2017  6:21-30  PubMed ID: 28293547   DOI: 10.2147/OV.S123292
6176  Mizugishi K, Yamashita K.  Neutrophil extracellular traps are critical for pregnancy loss in sphingosine kinase-deficient mice on 129Sv/C57BL/6 background.  FASEB J  2017  31(12):5577-5591  PubMed ID: 28842426   DOI: 10.1096/fj.201700399RR
14267  El-Naggar MH, Mira A, Abdel Bar FM, Shimizu K, Amer MM, Badria FA.  Synthesis, docking, cytotoxicity, and LTA 4 H inhibitory activity of new gingerol derivatives as potential colorectal cancer therapy  Bioorg Med Chem  2017  25(3):1277-128  PubMed ID: 28065501   DOI: 10.1016/j.bmc.2016.12.048
5669  Kwofie KD et al  Antitrypanosomal Activities and Mechanisms of Action of Novel Tetracyclic Iridoids from Morinda lucida Benth.  Antimicrob Agents Chemother  2016  60(6):3283-90  PubMed ID: 26953191   DOI: 10.1128/AAC.01916-15
3579  Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.  Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.  Stem Cells  2007  25(7):1610-7  PubMed ID: 17395773   DOI: 10.1634/stemcells.2006-0504
2824  Kakeda M, Hiratsuka M, Nagata K, Kuroiwa Y, Kakitani M, Katoh M, Oshimura M, Tomizuka K  Human artificial chromosome (HAC) vector provides long-term therapeutic transgene expression in normal human primary fibroblasts.  Gene Ther  2005  12:852-6  PubMed ID: 15750614   DOI: 10.1038/sj.gt.3302483



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