Cell No. : Cell Name
RCB0210 : HF19
update : 2023/05/25
|
| Comment | Normal human lung fibroblast. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 85011418 prior to order. |
| Comment from the depositor | |
| Terms and conditions | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85011418 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. |
| Remarks | |
| Order Form |
Order Form(C-0005.pdf)
MTA(C-0007.pdf)
MTA(C-0007p.pdf)
|
|
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
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Basic information
|
Depositor |
ECACC
|
| Year of deposit |
1987
|
| Animal |
_human
< Mammals
|
| Genus |
Homo
|
| Species |
sapiens
|
| Gender |
Female
|
| Age at sampling |
fetus
|
| Tissue |
lung
|
| Classification |
normal
|
| History |
overseas, ECACC
|
|
Lifespan |
finite
|
| Morphology |
fibroblast-like
|
| Cellosaurus(Expasy) |
CVCL_2487
|
|
| |
deposit info |
lot info |
|
Medium |
Medium List |
|
Culture type |
|
Adherent cells
|
| Culture medium |
|
HF-RITC80-7 (or MEM ALPHA) + 10% FBS
|
| Antibiotics |
|
Free
|
|
Passage method |
|
0.25% Trypsin
|
Culture information
|
Passage ratio |
|
1 : 4 split
|
| SC frequency |
|
Subculture : 2 times/week
|
| Temperature |
|
37
℃
|
| CO2 concentration |
|
5
%
|
| Freeze medium |
|
Medium + 10% DMSO
|
| Freezing method |
|
Slow freezing
|
| Mycoplasma/Acholeplasma |
|
(-)
|
| Virus (HIV) |
|
(-)
|
| Isozyme |
|
LD, NP
|
| Chromosome mode |
|
45-92(50) : 45(4),46(44),89(1),92(1)
|
| STR(human) |
|
OK
|
| Images |
deposit info | lot info |
|
|
|
| Reference information |
Reference |
2
|
| User's Publication |
9
|
| User's Publication |
|
20050
Yap JMG, Ueda T, Kanemitsu Y, Takeda N, Fukumitsu K, Fukuda S, Uemura T, Tajiri T, Ohkubo H, Maeno K, Ito Y, Oguri T, Ugawa S, Niimi A.
AITC inhibits fibroblast-myofibroblast transition via TRPA1-independent MAPK and NRF2/HO-1 pathways and reverses corticosteroids insensitivity in human lung fibroblasts.
Respir Res
2021
22(1):51
PubMed ID: 33579280
DOI: 10.1186/s12931-021-01636-9
|
|
12045
Yap JMG, Ueda T, Takeda N, Fukumitsu K, Fukuda S, Uemura T, Tajiri T, Ohkubo H, Maeno K, Ito Y, Kanemitsu Y, Niimi A.
An inflammatory stimulus sensitizes TRPA1 channel to increase cytokine release in human lung fibroblasts.
Cytokine
2020
PubMed ID: 32050145
DOI: 10.1016/j.cyto.2020.155027
|
|
12150
Amira Mira, Mohamed A. Sabry, Kuniyoshi Shimizu & Fatma M. Abdel Bar
A new pimarane-type diterpene obtained by biotransformation inhibits human HCT-116 colorectal carcinoma through inhibition of LTA4H activity
Original Research
2020
DOI: 10.1007/s00044-020-02520-9
|
|
5680
Ginting TE, Suryatenggara J, Christian S, Mathew G.
Proinflammatory response induced by Newcastle disease virus in tumor and normal cells.
Oncolytic Virother
2017
6:21-30
PubMed ID: 28293547
DOI: 10.2147/OV.S123292
|
|
6176
Mizugishi K, Yamashita K.
Neutrophil extracellular traps are critical for pregnancy loss in sphingosine kinase-deficient mice on 129Sv/C57BL/6 background.
FASEB J
2017
31(12):5577-5591
PubMed ID: 28842426
DOI: 10.1096/fj.201700399RR
|
|
14267
El-Naggar MH, Mira A, Abdel Bar FM, Shimizu K, Amer MM, Badria FA.
Synthesis, docking, cytotoxicity, and LTA 4 H inhibitory activity of new gingerol derivatives as potential colorectal cancer therapy
Bioorg Med Chem
2017
25(3):1277-128
PubMed ID: 28065501
DOI: 10.1016/j.bmc.2016.12.048
|
|
5669
Kwofie KD et al
Antitrypanosomal Activities and Mechanisms of Action of Novel Tetracyclic Iridoids from Morinda lucida Benth.
Antimicrob Agents Chemother
2016
60(6):3283-90
PubMed ID: 26953191
DOI: 10.1128/AAC.01916-15
|
|
3579
Sudo K, Kanno M, Miharada K, Ogawa S, Hiroyama T, Saijo K, Nakamura Y.
Mesenchymal progenitors able to differentiate into osteogenic, chondrogenic, and/or adipogenic cells in vitro are present in most primary fibroblast-like cell populations.
Stem Cells
2007
25(7):1610-7
PubMed ID: 17395773
DOI: 10.1634/stemcells.2006-0504
|
|
2824
Kakeda M, Hiratsuka M, Nagata K, Kuroiwa Y, Kakitani M, Katoh M, Oshimura M, Tomizuka K
Human artificial chromosome (HAC) vector provides long-term therapeutic transgene expression in normal human primary fibroblasts.
Gene Ther
2005
12:852-6
PubMed ID: 15750614
DOI: 10.1038/sj.gt.3302483
|
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