細胞番号 : 細胞名 RCB0209 : SP2/0-Ag14  update : 2023/05/25
細胞特性(Comment:英)			Mouse myeloma cell line. Utilized for generation of hybridoma. This is a non-Ig-secreting or synthesising line. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to the ECACC 85072401 prior to order.
細胞特性(日)			マウス由来ミエローマ細胞株。ハイブリドーマ作製用に使用される。免疫グロブリンを産生しない。ECACC由来株。使用条件等がECACCによって随時変更となる可能性があります。ご購入前に、ECACC 85072401 を必ずご参照ください。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)			1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85072401 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)			1) 基礎的研究につきましては、使用制限はありません。しかし、使用条件等がECACCによって随時変更となる可能性があるので、購入前に、ECA85072401 を必ず参照すること。2) 商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英)			Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)
			Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日)			依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf
			提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください（詳細）。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料			手数料とお支払いについてはこちらをご覧ください。
細胞基本情報		寄託者	ECACC
		樹立者	Shulman, M. et al
		寄託日	1987
		動物種	mouse < Mammals
		採取組織	myeloma
		病名	myeloma
		細胞分類	cancer
		入手歴	overseas, ECACC
		細胞寿命	infinite
		細胞形態	lymphocyte-like
		Cellosaurus(Expasy)	CVCL_2199
細胞培養・検査情報			寄託時情報	ロット情報
	培地・試薬情報		培地・試薬一覧はこちらをご覧ください。
		培養形態		Suspension cells
		培地		RPMI1640 + 10% FBS
		抗生物質		Free
		継代方法		dilution
		継代密度		1 : 10 split
		継代・培地交換頻度		Subculture : 2 times/week
		培養最適温度		37 ℃
		二酸化炭素濃度		5 %
		凍結培地		Medium + 10% DMSO
		凍結方法		Slow freezing
		マイコプラズマ		(-)
		マウス系統検査		OK
		アイソザイム検査		LD, NP
		染色体検査		60-74(50) : /67(4),68(8),69(8),70(5),71(8),72(3)/
画像情報			寄託時情報	ロット情報
文献情報		Reference(英)	3件
		Reference(日)	0件
		利用者成果(英)	27件
		利用者成果(日)	0件

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Reference(英)

4662  Ozato K, Sachs DH.  Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression.  J Immunol  1981  126(1):317-21  PubMed ID: 6935293

170  Köhler G.  Immunoglobulin chain loss in hybridoma lines  Proc Natl Acad Sci U S A  1980  77(4):2197-9  PubMed ID: 6769123   DOI: 10.1073/pnas.77.4.2197

335  Shulman M, Wilde, C D, Kohler G  A better cell line for making hybridomas secreting specific antibodies.  Nature  1978  276:269-70  PubMed ID: 714156   DOI: 10.1038/276269a0

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Reference(日)

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利用者成果(英)

13274  Juengsanguanpornsuk W, Yusakul G, Kitisripanya T, Krittanai S, Juengwatanatrakul T, Sakamoto S, Putalun W.  Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography.  J Pharm Biomed Anal  2021    PubMed ID: 33120305   DOI: 10.1016/j.jpba.2020.113674

13466  Guo Y, Kawaguchi A, Takeshita M, Sekiya T, Hirohama M, Yamashita A, Siomi H, Murano K.  Potent mouse monoclonal antibodies that block SARS-CoV-2 infection  J Biol Chem  2021  Jan 29  PubMed ID: 33524396   DOI: 10.1016/j.jbc.2021.100346

20210  Okuda T, Kitamara M, Kasahara S, Kato K.  Identification of genes for variable regions of immunoglobulins that recognize sialylated glycans.  Biochem Biophys Res Commun  2021  567:201-207  PubMed ID: 34166919   DOI: 10.1016/j.bbrc.2021.06.039

16696  Lin Y, Sidthipong K, Ma J, Koide N, Umezawa K, Kubota T.  The designed NF-κB inhibitor, DHMEQ, inhibits KISS1R-mediated invasion and increases drug-sensitivity in mouse plasmacytoma SP2/0 cells  Exp Ther Med  2021  22(4):1092  PubMed ID: 34504546   DOI: 10.3892/etm.2021.10526

16787  Yamasaki F, Umezawa F, Sensui T, Anzo M, Abo H, Kuo CW, Khoo KH, Kato K, Yagi H, Kawashima H.  Establishment of a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans  Biochem Biophys Res Commun  2021  579:8-14  PubMed ID: 34583196   DOI: 10.1016/j.bbrc.2021.09.043

12046  Endou M, Yoshida K, Hirota M, Nakajima C, Sakaguchi A, Komatsubara N, Kurihara Y.  Coxfa4l3, a novel mitochondrial electron transport chain Complex 4 subunit protein, switches from Coxfa4 during spermatogenesis.  Mitochondrion  2020    PubMed ID: 32045714   DOI: 10.1016/j.mito.2020.02.003

12275  Youjia Guo, Atsushi Kawaguchi, Masaru Takeshita, Takeshi Sekiya, Mikako Hirohama, Akio Yamashita, the Keio Donner Project, Haruhiko Siomi1, Kensaku Murano1  Potent mouse monoclonal antibodies that block SARS-CoV-2 infection  bioRxiv  2020  October 2

13497  Okuda T.  Isolation and Characterization of Antibodies Induced by Immunization with TNF-α Inducible Globotetraosylceramide  Int J Mol Sci  2020  21(10):3632  PubMed ID: 32455599   DOI: 10.3390/ijms21103632

11833  Hiyoshi Y, Sato Y, Ichinoe M, Nagashio R, Hagiuda D, Kobayashi M, Kusuhara S, Igawa S, Shiomi K, Goshima N, Murakumo Y, Saegusa M, Satoh Y, Masuda N, Naoki K.  Prognostic significance of IMMT expression in surgically-resected lung adenocarcinoma.  Thorac Cancer  2019    PubMed ID: 31583841   DOI: 10.1111/1759-7714.13200

10621  Akagi S, Nakajima C, Tanaka Y, Kurihara Y.  Flow cytometry-based method for rapid and high-throughput screening of hybridoma cells secreting monoclonal antibody.  J. Biosci. Bioeng.  2018  125:464-469  PubMed ID: 29174537   DOI: 10.1016/j.jbiosc.2017.10.012

10753  Okuda T, Fukui A.  Generation of anti-oligosaccharide antibodies that recognize mammalian glycoproteins by immunization with a novel artificial glycosphingolipid.  Biochem. Biophys. Res. Commun.  2018  497:983-989  PubMed ID: 29458022   DOI: 10.1016/j.bbrc.2018.02.113

11070  Okuda T.  Data on immunoglobulin G antibodies induced by immunization of mice with globoside carrying very long-chain fatty acids  Data Brief  2018  19:256-260  PubMed ID: 29892643   DOI: 10.1016/j.dib.2018.05.014

4454  Hayashi Y, Okutani M, Ogawa S, Tsukahara T, Inoue R.  Generation of anti-porcine CD69 monoclonal antibodies and their usefulness to evaluate early activation of cellular immunity by flow cytometric analysis.  Anim. Sci. J.  2018  89:825-832  PubMed ID: 29460471   DOI: 10.1111/asj.12989

10249  Okuda T.  PUGNAc treatment provokes globotetraosylceramide accumulation in human umbilical vein endothelial cells.  Biochem. Biophys. Res. Commun.  2017  487:76-82  PubMed ID: 28392398   DOI: 10.1016/j.bbrc.2017.04.019

14302  Kitisripanya T, Inyai C, Krittanai S, Likhitwitayawuid K, Sritularak B, Ploypradith P, Tanaka H, Morimoto S, Putalun W.  A monoclonal antibody-based immunoassay for the determination of oxyresveratrol from Artocarpus lacucha Buch.-Ham  J Nat Med  2017  71(3):523-530  PubMed ID: 28364228   DOI: 10.1007/s11418-017-1083-8

14303  Ofuji K, Saito K, Suzuki S, Shimomura M, Shirakawa H, Nobuoka D, Sawada Y, Yoshimura M, Tsuchiya N, Takahashi M, Yoshikawa T, Tada Y, Konishi M, Takahashi S, Gotohda N, Nakamoto Y, Nakatsura T.  Perioperative plasma glypican-3 level may enable prediction of the risk of recurrence after surgery in patients with stage I hepatocellular carcinoma  Oncotarget  2017  8(23):37835-37844  PubMed ID: 28035063   DOI: 10.18632/oncotarget.14271

7490  Ohsawa H, Baba T, Enami J, Hiramatsu K.  Successful selection of an infection-protective anti-Staphylococcus aureus monoclonal antibody and its protective activity in murine infection models.  Microbiol. Immunol.  2015  59:183-92  PubMed ID: 25659598   DOI: 10.1111/1348-0421.12242

14286  Katono K, Sato Y, Jiang SX, Kobayashi M, Nagashio R, Ryuge S, Fukuda E, Goshima N, Satoh Y, Saegusa M, Masuda N.  Prognostic significance of MYH9 expression in resected non-small cell lung cancer  PLoS One  2015  10(3):e0121460  PubMed ID: 25826333   DOI: 10.1371/journal.pone.0121460

15443  Tanizaki Y, Ishida-Iwata T, Obuchi-Shimoji M, Kato T.  Cellular characterization of thrombocytes in Xenopus laevis with specific monoclonal antibodies  Exp Hematol  2015  43(2):125-36  PubMed ID: 25448492   DOI: 10.1016/j.exphem.2014.10.005

9108  Magot Diata Omokoko, Sabar Pambudi, Supranee Phanthanawiboon, Promsin Masrinoul, Chayanee Setthapramote, Tadahiro Sasaki, Motoki Kuhara, Pongrama Ramasoota, Akifumi Yamashita, Itaru Hirai, Kazuyoshi Ikuta, Takeshi Kurosu  A Highly Conserved Region Between Amino Acids 221 and 266 of Dengue Virus Non-Structural Protein 1 Is a Major Epitope Region in Infected Patients  Am J Trop Med Hyg  2014  91 (1), 146-55  PubMed ID: 24778195   DOI: 10.4269/ajtmh.13-0624

14287  Kobayashi M, Matsumoto T, Ryuge S, Yanagita K, Nagashio R, Kawakami Y, Goshima N, Jiang SX, Saegusa M, Iyoda A, Satoh Y, Masuda N, Sato Y.  CAXII Is a sero-diagnostic marker for lung cancer  PLoS One  2012  7(3):e33952  PubMed ID: 22439015   DOI: 10.1371/journal.pone.0033952

8541  Kubota-Koketsu R, Mizuta H, Oshita M, Ideno S, Yunoki M, Kuhara M, Yamamoto N, Okuno Y, Ikuta K.  Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors.  Biochem. Biophys. Res. Commun.  2009  387:180-5  PubMed ID: 19580789   DOI: 10.1016/j.bbrc.2009.06.151

8543  Kiyohara T, Totsuka A, Yoneyama T, Ishii K, Ito T, Wakita T.  Characterization of anti-idiotypic antibodies mimicking antibody- and receptor-binding sites on hepatitis A virus.  Arch. Virol.  2009  154:1263-9  PubMed ID: 19578927   DOI: 10.1007/s00705-009-0433-6

14288  Morikawa A, Hamase K, Ohgusu T, Etoh S, Tanaka H, Koshiishi I, Shoyama Y, Zaitsu K.  Immunohistochemical localization of D-alanine to beta-cells in rat pancreas  Biochem Biophys Res Commun  2007  355(4):872-6  PubMed ID: 17336268   DOI: 10.1016/j.bbrc.2007.02.056

14290  Iwabata H, Yoshida M, Komatsu Y.  Proteomic analysis of organ-specific post-translational lysine-acetylation and -methylation in mice by use of anti-acetyllysine and -methyllysine mouse monoclonal antibodies  Proteomics  2005  5(18):4653-64  PubMed ID: 16247734   DOI: 10.1002/pmic.200500042

5959  Komatsu Y, Tomizaki KY, Tsukamoto M, Kato T, Nishino N, Sato S, Yamori T, Tsuruo T, Furumai R, Yoshida M, Horinouchi S, Hayashi H.  Cyclic hydroxamic-acid-containing peptide 31, a potent synthetic histone deacetylase inhibitor with antitumor activity.  Cancer Res  2001  61(11):4459-66  PubMed ID: 11389076

15569  Maki Murata-Hori, Takashi Uchimura, Natsumi Hosoya, Takahiro Iwasaki, Saburo Omata, Tsuneyoshi Horigome, Hiroshi Hosoya  A Monoclonal Antibody Raised Against an 86-kD Subunit of Human DNA Helicase II  Zoological Science  2000  17:1255-1259    DOI: 10.2108/zsj.17.1255

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