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Cell No. : Cell Name
RCB0209 : SP2/0-Ag14  update : 2024/01/17
CommentMouse myeloma cell line. Utilized for generation of hybridoma. This is a non-Ig-secreting or synthesising line. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to the ECACC 85072401 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85072401 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor ECACC
Originator Shulman, M. et al
Year of deposit 1987
Animal _mouse < Mammals
Tissue myeloma
Disease name myeloma
Classification cancer
History overseas, ECACC
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2199
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture medium RPMI1640 + 10% FBS
Culture method
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 60-74(50) : /67(4),68(8),69(8),70(5),71(8),72(3)/
deposit info
lot info
Reference information Reference 3
User's Publication 33

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4662  Ozato K, Sachs DH.  Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression.  J Immunol  1981  126(1):317-21  PubMed ID: 6935293  
170  Köhler G.  Immunoglobulin chain loss in hybridoma lines  Proc Natl Acad Sci U S A  1980  77(4):2197-9  PubMed ID: 6769123   DOI: 10.1073/pnas.77.4.2197
335  Shulman M, Wilde, C D, Kohler G  A better cell line for making hybridomas secreting specific antibodies.  Nature  1978  276:269-70  PubMed ID: 714156   DOI: 10.1038/276269a0

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User's Publication
21431  Suzuki Y, Takai S, Morizane Y, Yasuda K, Takahashi K, Ishitsuka T, Sasaki Y, Otsuka M, Kato S, Madarame H, Sugiyama M, Kawaguchi H, Kakuda T.  Development of monoclonal antibodies against Rhodococcus equi virulence-associated protein N and their application to pathological diagnosis.  Microbiol Spectr  2023  e0072923  PubMed ID: 37800907   DOI: 10.1128/spectrum.00729-23
21437  Ochi A, Fujii S, Ohta T, Uto T, Nuntawong P, Tanaka H, Morimoto S, Sakamoto S.  Highly sensitive indirect competitive enzyme-linked immunosorbent assay based on a monoclonal antibody against saikosaponin b2 for quality control of Kampo medicines containing Bupleuri radix.  J Nat Med  2023    PubMed ID: 37804411   DOI: 10.1007/s11418-023-01753-3
21478  Sae-Foo W, Singkham S, Srisongkhram P, Yusakul G, Masugarut P, Putalun W.  Development and characterisation of highly specific monoclonal antibody-based immunoassays for the detection and quantification of genistein-7-O-[α-rhamnopyranosyl-(1→6)]-β-glucopyranoside in Derris scandens (Roxb.) Benth.  Phytochem Anal  2023    PubMed ID: 37965872   DOI: 10.1002/pca.3305
13274  Juengsanguanpornsuk W, Yusakul G, Kitisripanya T, Krittanai S, Juengwatanatrakul T, Sakamoto S, Putalun W.  Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography.  J Pharm Biomed Anal  2021    PubMed ID: 33120305   DOI: 10.1016/j.jpba.2020.113674
13466  Guo Y, Kawaguchi A, Takeshita M, Sekiya T, Hirohama M, Yamashita A, Siomi H, Murano K.  Potent mouse monoclonal antibodies that block SARS-CoV-2 infection  J Biol Chem  2021  Jan 29  PubMed ID: 33524396   DOI: 10.1016/j.jbc.2021.100346
20210  Okuda T, Kitamara M, Kasahara S, Kato K.  Identification of genes for variable regions of immunoglobulins that recognize sialylated glycans.  Biochem Biophys Res Commun  2021  567:201-207  PubMed ID: 34166919   DOI: 10.1016/j.bbrc.2021.06.039
16696  Lin Y, Sidthipong K, Ma J, Koide N, Umezawa K, Kubota T.  The designed NF-κB inhibitor, DHMEQ, inhibits KISS1R-mediated invasion and increases drug-sensitivity in mouse plasmacytoma SP2/0 cells  Exp Ther Med  2021  22(4):1092  PubMed ID: 34504546   DOI: 10.3892/etm.2021.10526
16787  Yamasaki F, Umezawa F, Sensui T, Anzo M, Abo H, Kuo CW, Khoo KH, Kato K, Yagi H, Kawashima H.  Establishment of a novel monoclonal antibody against truncated glycoforms of α-dystroglycan lacking matriglycans  Biochem Biophys Res Commun  2021  579:8-14  PubMed ID: 34583196   DOI: 10.1016/j.bbrc.2021.09.043
12046  Endou M, Yoshida K, Hirota M, Nakajima C, Sakaguchi A, Komatsubara N, Kurihara Y.  Coxfa4l3, a novel mitochondrial electron transport chain Complex 4 subunit protein, switches from Coxfa4 during spermatogenesis.  Mitochondrion  2020    PubMed ID: 32045714   DOI: 10.1016/j.mito.2020.02.003
13497  Okuda T.  Isolation and Characterization of Antibodies Induced by Immunization with TNF-α Inducible Globotetraosylceramide  Int J Mol Sci  2020  21(10):3632  PubMed ID: 32455599   DOI: 10.3390/ijms21103632
11833  Hiyoshi Y, Sato Y, Ichinoe M, Nagashio R, Hagiuda D, Kobayashi M, Kusuhara S, Igawa S, Shiomi K, Goshima N, Murakumo Y, Saegusa M, Satoh Y, Masuda N, Naoki K.  Prognostic significance of IMMT expression in surgically-resected lung adenocarcinoma.  Thorac Cancer  2019    PubMed ID: 31583841   DOI: 10.1111/1759-7714.13200
10621  Akagi S, Nakajima C, Tanaka Y, Kurihara Y.  Flow cytometry-based method for rapid and high-throughput screening of hybridoma cells secreting monoclonal antibody.  J. Biosci. Bioeng.  2018  125:464-469  PubMed ID: 29174537   DOI: 10.1016/j.jbiosc.2017.10.012
10753  Okuda T, Fukui A.  Generation of anti-oligosaccharide antibodies that recognize mammalian glycoproteins by immunization with a novel artificial glycosphingolipid.  Biochem. Biophys. Res. Commun.  2018  497:983-989  PubMed ID: 29458022   DOI: 10.1016/j.bbrc.2018.02.113
11070  Okuda T.  Data on immunoglobulin G antibodies induced by immunization of mice with globoside carrying very long-chain fatty acids  Data Brief  2018  19:256-260  PubMed ID: 29892643   DOI: 10.1016/j.dib.2018.05.014
4454  Hayashi Y, Okutani M, Ogawa S, Tsukahara T, Inoue R.  Generation of anti-porcine CD69 monoclonal antibodies and their usefulness to evaluate early activation of cellular immunity by flow cytometric analysis.  Anim. Sci. J.  2018  89:825-832  PubMed ID: 29460471   DOI: 10.1111/asj.12989
10249  Okuda T.  PUGNAc treatment provokes globotetraosylceramide accumulation in human umbilical vein endothelial cells.  Biochem. Biophys. Res. Commun.  2017  487:76-82  PubMed ID: 28392398   DOI: 10.1016/j.bbrc.2017.04.019
14302  Kitisripanya T, Inyai C, Krittanai S, Likhitwitayawuid K, Sritularak B, Ploypradith P, Tanaka H, Morimoto S, Putalun W.  A monoclonal antibody-based immunoassay for the determination of oxyresveratrol from Artocarpus lacucha Buch.-Ham  J Nat Med  2017  71(3):523-530  PubMed ID: 28364228   DOI: 10.1007/s11418-017-1083-8
14303  Ofuji K, Saito K, Suzuki S, Shimomura M, Shirakawa H, Nobuoka D, Sawada Y, Yoshimura M, Tsuchiya N, Takahashi M, Yoshikawa T, Tada Y, Konishi M, Takahashi S, Gotohda N, Nakamoto Y, Nakatsura T.  Perioperative plasma glypican-3 level may enable prediction of the risk of recurrence after surgery in patients with stage I hepatocellular carcinoma  Oncotarget  2017  8(23):37835-37844  PubMed ID: 28035063   DOI: 10.18632/oncotarget.14271
7490  Ohsawa H, Baba T, Enami J, Hiramatsu K.  Successful selection of an infection-protective anti-Staphylococcus aureus monoclonal antibody and its protective activity in murine infection models.  Microbiol. Immunol.  2015  59:183-92  PubMed ID: 25659598   DOI: 10.1111/1348-0421.12242
14286  Katono K, Sato Y, Jiang SX, Kobayashi M, Nagashio R, Ryuge S, Fukuda E, Goshima N, Satoh Y, Saegusa M, Masuda N.  Prognostic significance of MYH9 expression in resected non-small cell lung cancer  PLoS One  2015  10(3):e0121460  PubMed ID: 25826333   DOI: 10.1371/journal.pone.0121460
15443  Tanizaki Y, Ishida-Iwata T, Obuchi-Shimoji M, Kato T.  Cellular characterization of thrombocytes in Xenopus laevis with specific monoclonal antibodies  Exp Hematol  2015  43(2):125-36  PubMed ID: 25448492   DOI: 10.1016/j.exphem.2014.10.005
9108  Magot Diata Omokoko, Sabar Pambudi, Supranee Phanthanawiboon, Promsin Masrinoul, Chayanee Setthapramote, Tadahiro Sasaki, Motoki Kuhara, Pongrama Ramasoota, Akifumi Yamashita, Itaru Hirai, Kazuyoshi Ikuta, Takeshi Kurosu  A Highly Conserved Region Between Amino Acids 221 and 266 of Dengue Virus Non-Structural Protein 1 Is a Major Epitope Region in Infected Patients  Am J Trop Med Hyg  2014  91 (1), 146-55  PubMed ID: 24778195   DOI: 10.4269/ajtmh.13-0624
14287  Kobayashi M, Matsumoto T, Ryuge S, Yanagita K, Nagashio R, Kawakami Y, Goshima N, Jiang SX, Saegusa M, Iyoda A, Satoh Y, Masuda N, Sato Y.  CAXII Is a sero-diagnostic marker for lung cancer  PLoS One  2012  7(3):e33952  PubMed ID: 22439015   DOI: 10.1371/journal.pone.0033952
8541  Kubota-Koketsu R, Mizuta H, Oshita M, Ideno S, Yunoki M, Kuhara M, Yamamoto N, Okuno Y, Ikuta K.  Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors.  Biochem. Biophys. Res. Commun.  2009  387:180-5  PubMed ID: 19580789   DOI: 10.1016/j.bbrc.2009.06.151
8543  Kiyohara T, Totsuka A, Yoneyama T, Ishii K, Ito T, Wakita T.  Characterization of anti-idiotypic antibodies mimicking antibody- and receptor-binding sites on hepatitis A virus.  Arch. Virol.  2009  154:1263-9  PubMed ID: 19578927   DOI: 10.1007/s00705-009-0433-6
18336  Kido K, Morinaga O, Shoyama Y, Tanaka H.  Quick analysis of baicalin in Scutellariae Radix by enzyme-linked immunosorbent assay using a monoclonal antibody  Talanta  2008  77(1):346-50  PubMed ID: 18804644   DOI: 10.1016/j.talanta.2008.06.034
14288  Morikawa A, Hamase K, Ohgusu T, Etoh S, Tanaka H, Koshiishi I, Shoyama Y, Zaitsu K.  Immunohistochemical localization of D-alanine to beta-cells in rat pancreas  Biochem Biophys Res Commun  2007  355(4):872-6  PubMed ID: 17336268   DOI: 10.1016/j.bbrc.2007.02.056
18626  Ohgusu T, Hamase K, Tanaka H, Shoyama Y, Zaitsu K.  High-throughput determination of free D-aspartic acid in mammals by enzyme immunoassay using specific monoclonal antibody  Anal Biochem  2006  357(1):15-20  PubMed ID: 16920063   DOI: 10.1016/j.ab.2006.06.039
14290  Iwabata H, Yoshida M, Komatsu Y.  Proteomic analysis of organ-specific post-translational lysine-acetylation and -methylation in mice by use of anti-acetyllysine and -methyllysine mouse monoclonal antibodies  Proteomics  2005  5(18):4653-64  PubMed ID: 16247734   DOI: 10.1002/pmic.200500042
18642  Komatsu Y, Yukutake Y, Yoshida M.  Four different clones of mouse anti-acetyllysine monoclonal antibodies having different recognition properties share a common immunoglobulin framework structure  J Immunol Methods  2003  272(1-2):161-75  PubMed ID: 12505721   DOI: 10.1016/s0022-1759(02)00500-8
5959  Komatsu Y, Tomizaki KY, Tsukamoto M, Kato T, Nishino N, Sato S, Yamori T, Tsuruo T, Furumai R, Yoshida M, Horinouchi S, Hayashi H.  Cyclic hydroxamic-acid-containing peptide 31, a potent synthetic histone deacetylase inhibitor with antitumor activity.  Cancer Res  2001  61(11):4459-66  PubMed ID: 11389076  
15569  Maki Murata-Hori, Takashi Uchimura, Natsumi Hosoya, Takahiro Iwasaki, Saburo Omata, Tsuneyoshi Horigome, Hiroshi Hosoya  A Monoclonal Antibody Raised Against an 86-kD Subunit of Human DNA Helicase II  Zoological Science  2000  17:1255-1259    DOI: 10.2108/zsj.17.1255
17912  Kozaki K, Miyaishi O, Asai N, Iida K, Sakata K, Hayashi M, Nishida T, Matsuyama M, Shimizu S, Kaneda T.  Tissue distribution of ERp61 and association of its increased expression with IgG production in hybridoma cells  Exp Cell Res  1994  213(2):348-58  PubMed ID: 8050492   DOI: 10.1006/excr.1994.1209

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