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Cell No. : Cell Name
RCB0209 : SP2/0-Ag14  update : 2021/09/17
CommentMouse myeloma cell line. Utilized for generation of hybridoma. This is a non-Ig-secreting or synthesising line. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to the ECA 85072401 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85072401 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor ECACC
Originator Shulman, M. et al
Year of deposit 1987
Animal _mouse < Mammals
Tissue myeloma
Disease name myeloma
Classification cancer
History overseas, ECACC
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_2199
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Medium and additives RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 60-74(50) : /67(4),68(8),69(8),70(5),71(8),72(3)/
deposit info
lot info
Reference information Reference 2
User's Publication 25

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4662  Ozato K, Sachs DH.  Monoclonal antibodies to mouse MHC antigens. III. Hybridoma antibodies reacting to antigens of the H-2b haplotype reveal genetic control of isotype expression.  J Immunol  1981  126(1):317-21  PubMed ID: 6935293
335  Shulman M, Wilde, C D, Kohler G  A better cell line for making hybridomas secreting specific antibodies.  Nature  1978  276:269-70  PubMed ID: 714156

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User's Publication
13274  Juengsanguanpornsuk W, Yusakul G, Kitisripanya T, Krittanai S, Juengwatanatrakul T, Sakamoto S, Putalun W.  Quantification of methylisomiroestrol, a phytoestrogen of Pueraria candollei, by enzyme-linked immunosorbent assay in comparison with high-performance liquid chromatography.  J Pharm Biomed Anal  2021    PubMed ID: 33120305
13466  Guo Y, Kawaguchi A, Takeshita M, Sekiya T, Hirohama M, Yamashita A, Siomi H, Murano K.  Potent mouse monoclonal antibodies that block SARS-CoV-2 infection  J Biol Chem  2021  Jan 29  PubMed ID: 33524396
20210  Okuda T, Kitamara M, Kasahara S, Kato K.  Identification of genes for variable regions of immunoglobulins that recognize sialylated glycans.  Biochem Biophys Res Commun  2021  567:201-207  PubMed ID: 34166919
12046  Endou M, Yoshida K, Hirota M, Nakajima C, Sakaguchi A, Komatsubara N, Kurihara Y.  Coxfa4l3, a novel mitochondrial electron transport chain Complex 4 subunit protein, switches from Coxfa4 during spermatogenesis.  Mitochondrion  2020    PubMed ID: 32045714
12275  Youjia Guo, Atsushi Kawaguchi, Masaru Takeshita, Takeshi Sekiya, Mikako Hirohama, Akio Yamashita, the Keio Donner Project, Haruhiko Siomi1, Kensaku Murano1  Potent mouse monoclonal antibodies that block SARS-CoV-2 infection  bioRxiv  2020  October 2 
13497  Okuda T.  Isolation and Characterization of Antibodies Induced by Immunization with TNF-α Inducible Globotetraosylceramide  Int J Mol Sci  2020  21(10):3632  PubMed ID: 32455599
11833  Hiyoshi Y, Sato Y, Ichinoe M, Nagashio R, Hagiuda D, Kobayashi M, Kusuhara S, Igawa S, Shiomi K, Goshima N, Murakumo Y, Saegusa M, Satoh Y, Masuda N, Naoki K.  Prognostic significance of IMMT expression in surgically-resected lung adenocarcinoma.  Thorac Cancer  2019    PubMed ID: 31583841
10621  Akagi S, Nakajima C, Tanaka Y, Kurihara Y.  Flow cytometry-based method for rapid and high-throughput screening of hybridoma cells secreting monoclonal antibody.  J. Biosci. Bioeng.  2018  125:464-469  PubMed ID: 29174537
10753  Okuda T, Fukui A.  Generation of anti-oligosaccharide antibodies that recognize mammalian glycoproteins by immunization with a novel artificial glycosphingolipid.  Biochem. Biophys. Res. Commun.  2018  497:983-989  PubMed ID: 29458022
11070  Okuda T.  Data on immunoglobulin G antibodies induced by immunization of mice with globoside carrying very long-chain fatty acids  Data Brief  2018  19:256-260  PubMed ID: 29892643
4454  Hayashi Y, Okutani M, Ogawa S, Tsukahara T, Inoue R.  Generation of anti-porcine CD69 monoclonal antibodies and their usefulness to evaluate early activation of cellular immunity by flow cytometric analysis.  Anim. Sci. J.  2018  89:825-832  PubMed ID: 29460471
10249  Okuda T.  PUGNAc treatment provokes globotetraosylceramide accumulation in human umbilical vein endothelial cells.  Biochem. Biophys. Res. Commun.  2017  487:76-82  PubMed ID: 28392398
14302  Kitisripanya T, Inyai C, Krittanai S, Likhitwitayawuid K, Sritularak B, Ploypradith P, Tanaka H, Morimoto S, Putalun W.  A monoclonal antibody-based immunoassay for the determination of oxyresveratrol from Artocarpus lacucha Buch.-Ham  J Nat Med  2017  71(3):523-530  PubMed ID: 28364228
14303  Ofuji K, Saito K, Suzuki S, Shimomura M, Shirakawa H, Nobuoka D, Sawada Y, Yoshimura M, Tsuchiya N, Takahashi M, Yoshikawa T, Tada Y, Konishi M, Takahashi S, Gotohda N, Nakamoto Y, Nakatsura T.  Perioperative plasma glypican-3 level may enable prediction of the risk of recurrence after surgery in patients with stage I hepatocellular carcinoma  Oncotarget  2017  8(23):37835-37844  PubMed ID: 28035063
7490  Ohsawa H, Baba T, Enami J, Hiramatsu K.  Successful selection of an infection-protective anti-Staphylococcus aureus monoclonal antibody and its protective activity in murine infection models.  Microbiol. Immunol.  2015  59:183-92  PubMed ID: 25659598
14286  Katono K, Sato Y, Jiang SX, Kobayashi M, Nagashio R, Ryuge S, Fukuda E, Goshima N, Satoh Y, Saegusa M, Masuda N.  Prognostic significance of MYH9 expression in resected non-small cell lung cancer  PLoS One  2015  10(3):e0121460  PubMed ID: 25826333
15443  Tanizaki Y, Ishida-Iwata T, Obuchi-Shimoji M, Kato T.  Cellular characterization of thrombocytes in Xenopus laevis with specific monoclonal antibodies  Exp Hematol  2015  43(2):125-36  PubMed ID: 25448492
9108  Magot Diata Omokoko, Sabar Pambudi, Supranee Phanthanawiboon, Promsin Masrinoul, Chayanee Setthapramote, Tadahiro Sasaki, Motoki Kuhara, Pongrama Ramasoota, Akifumi Yamashita, Itaru Hirai, Kazuyoshi Ikuta, Takeshi Kurosu  A Highly Conserved Region Between Amino Acids 221 and 266 of Dengue Virus Non-Structural Protein 1 Is a Major Epitope Region in Infected Patients  Am J Trop Med Hyg  2014  91 (1), 146-55  PubMed ID: 24778195
14287  Kobayashi M, Matsumoto T, Ryuge S, Yanagita K, Nagashio R, Kawakami Y, Goshima N, Jiang SX, Saegusa M, Iyoda A, Satoh Y, Masuda N, Sato Y.  CAXII Is a sero-diagnostic marker for lung cancer  PLoS One  2012  7(3):e33952  PubMed ID: 22439015
8541  Kubota-Koketsu R, Mizuta H, Oshita M, Ideno S, Yunoki M, Kuhara M, Yamamoto N, Okuno Y, Ikuta K.  Broad neutralizing human monoclonal antibodies against influenza virus from vaccinated healthy donors.  Biochem. Biophys. Res. Commun.  2009  387:180-5  PubMed ID: 19580789
8543  Kiyohara T, Totsuka A, Yoneyama T, Ishii K, Ito T, Wakita T.  Characterization of anti-idiotypic antibodies mimicking antibody- and receptor-binding sites on hepatitis A virus.  Arch. Virol.  2009  154:1263-9  PubMed ID: 19578927
14290  Iwabata H, Yoshida M, Komatsu Y.  Proteomic analysis of organ-specific post-translational lysine-acetylation and -methylation in mice by use of anti-acetyllysine and -methyllysine mouse monoclonal antibodies  Proteomics  2005  5(18):4653-64  PubMed ID: 16247734
5959  Komatsu Y, Tomizaki KY, Tsukamoto M, Kato T, Nishino N, Sato S, Yamori T, Tsuruo T, Furumai R, Yoshida M, Horinouchi S, Hayashi H.  Cyclic hydroxamic-acid-containing peptide 31, a potent synthetic histone deacetylase inhibitor with antitumor activity.  Cancer Res  2001  61(11):4459-66  PubMed ID: 11389076
14288  Morikawa A, Hamase K, Ohgusu T, Etoh S, Tanaka H, Koshiishi I, Shoyama Y, Zaitsu K.  Immunohistochemical localization of D-alanine to beta-cells in rat pancreas  Biochem Biophys Res Commun  2007  355(4):872-6  PubMed ID: 17336268
15569  Maki Murata-Hori, Takashi Uchimura, Natsumi Hosoya, Takahiro Iwasaki, Saburo Omata, Tsuneyoshi Horigome, Hiroshi Hosoya  A Monoclonal Antibody Raised Against an 86-kD Subunit of Human DNA Helicase II  Zoological Science    17:1255-1259 

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