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Cell No. : Cell Name
RCB0208 : LTK-  update : 2022/03/08
CommentThymidine kinase defective L cell. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85011432 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85011432 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ECACC
Year of deposit 1987
Animal mouse < Mammals
Tissue connective tissue
Classification mutant
History overseas
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_3392
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 1-2 times/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
SSLP(mouse) OK
Isozyme LD, NP
Chromosome mode 47-62(50) : 47(2),48(10),49(31),50(5),51(1),62(1)
Reference information Reference 1
User's Publication 6


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Reference
955  Wigler M, Silverstein S, Lee, L S, Pellicer A, Cheng, Y c, Axel R  Transfer of purified herpes virus thymidine kinase gene to cultured mouse cells.  Cell  1977  11:223-32  PubMed ID: 194704

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User's Publication
7682  Bidet M, Joubert O, Lacombe B, Ciantar M, Nehme R, Mollat P, Bretillon L, Faure H, Bittman R, Ruat M, Mus-Veteau I.  The hedgehog receptor patched is involved in cholesterol transport.  PLoS ONE  2011  6:e23834  PubMed ID: 21931618
2375  Shimoyama Y, Takeda H, Yoshihara S, Kitajima M, Hirohashi S  Biochemical characterization and functional analysis of two type II classic cadherins, cadherin-6 and -14, and comparison with E-cadherin.  J Biol Chem  1999  274:11987-94  PubMed ID: 10207020
3970  Kashima T, Kawaguchi J, Takeshita S, Kuroda M, Takanashi M, Horiuchi H, Imamura T, Ishikawa Y, Ishida T, Mori S, Machinami R, Kudo A.  Anomalous cadherin expression in osteosarcoma. Possible relationships to metastasis and morphogenesis.  Am J Pathol  1999  155(5):1549-55  PubMed ID: 10550312
5970  Yoshida K, Watanabe M, Kato H, Dutta A, Sugano S.  BH-protocadherin-c, a member of the cadherin superfamily, interacts with protein phosphatase 1 alpha through its intracellular domain.  FEBS Lett  1999  460(1):93-8  PubMed ID: 10571067
2374  Shimoyama Y, Shibata T, Kitajima M, Hirohashi S  Molecular cloning and characterization of a novel human classic cadherin homologous with mouse muscle cadherin.  J Biol Chem  1998  273:10011-8  PubMed ID: 9545347
17225  Fukushige S, Ikeda JE.  Trapping of mammalian promoters by Cre-lox site-specific recombination  DNA Res  1996  3(2):73-80  PubMed ID: 8804858



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