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細胞番号 : 細胞名
RCB0207 : MRC-5 SV1 TG1  update : 2023/05/25
細胞特性(Comment:英)SV40-transformed, 6TG-resistant and HAT-sensitive. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 85042501 prior to order.
細胞特性(日)MRC-5由来SV40 transformantの一つで、6-thioguanine耐性、HAT感受性株である。ECACC由来株。使用条件等がECACCによって随時変更となる可能性があります。ご購入前に、ECACC 85042501 を必ずご参照ください。
使用条件(英)1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85042501 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)1) 基礎的研究につきましては、使用制限はありません。しかし、使用条件等がECACCによって随時変更となる可能性があるので、購入前に、ECA85042501 を必ず参照すること。2) 商業利用や特許取得を目的とする場合には、事前に必ず理研細胞バンクに連絡をすること。
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず cellbank.brc@riken.jp までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 ECACC
樹立者 Jacobs, P.
寄託日 1987
元の細胞 MRC-5
動物種 _human < Mammals
属名 Homo
種名 sapiens
性別 Male
採取組織 lung
細胞分類 transformed
入手歴 overseas, ECACC
細胞寿命 infinite
細胞形態 fibroblast-like
Cellosaurus(Expasy) CVCL_2625
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 HF-RITC80-7 (or MEM ALPHA) + 10% FBS
抗生物質 Free
継代方法 0.25% Trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
ウィルス(HIV) (-)
アイソザイム検査 LD, NP
染色体検査 71-145(50) : /74(3),75(3),76(8),77(12),78(10),79(4)/
個体識別検査 OK
文献情報 Reference(英) 2件
Reference(日) 0件
利用者成果(英) 18件
利用者成果(日) 0件

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