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Cell No. : Cell Name
RCB0207 : MRC-5 SV1 TG1  update : 2022/03/08
CommentSV40-transformed, 6TG-resistant and HAT-sensitive. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85042501 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85042501 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ECACC
Originator Jacobs, P.
Year of deposit 1987
Original cell MRC-5
Animal human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling fetus
Tissue lung
Classification transformed
History overseas, ECACC
Lifespan infinite
Morphology fibroblast-like
Cellosaurus(Expasy) CVCL_2625
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives HF-RITC80-7 (or MEM ALPHA) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP
Chromosome mode 71-145(50) : /74(3),75(3),76(8),77(12),78(10),79(4)/
STR(human) OK
Reference information Reference 2
User's Publication 16


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Reference
1033  T Matsumura 1, F Malik, R Holliday  Levels of DNA methylation in diploid and SV40 transformed human fibroblasts  Exp Gerontol  1989  24(5-6):477-81  PubMed ID: 2561103
1028  Jacobs, J P, Jones, C M, Baille, J P  Characteristics of a human diploid cell designated MRC-5.  Nature  1970  227:168-70  PubMed ID: 4316953

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User's Publication
16512  Li S, Kawashima D, Sugawara M, Obara H, Okeyo KO, Takei M.  Study of transmembrane ion transport under tonicity imbalance using a combination of low frequency-electrical impedance spectroscopy (LF-EIS) and improved ion transport model  Biomed Phys Eng Express  2022  8(3)  PubMed ID: 35316798
13256  Sakamoto Y, Kokuta T, Teshigahara A, Iijima K, Kitao H, Takata M, Tauchi H.  Mitotic cells can repair DNA double-strand breaks via a homology-directed pathway.  J Radiat Res  2021    PubMed ID: 33009557
14540  Sato N, Yao J, Sugawara M, Takei M.  Numerical Study of Particle-Fluid Flow Under AC Electrokinetics in Electrode-Multilayered Microfluidic Device  IEEE Trans Biomed Eng  2019  66(2):453-463  PubMed ID: 29993454
10767  Kuragano M, Uyeda TQP, Kamijo K, Murakami Y, Takahashi M.  Different contributions of nonmuscle myosin IIA and IIB to the organization of stress fiber subtypes in fibroblasts.  Mol. Biol. Cell  2018  29:911-922  PubMed ID: 29467250
10310  Sato Y, Murakami Y, Takahashi M.  Semi-retentive cytoskeletal fractionation (SERCYF): A novel method for the biochemical analysis of the organization of microtubule and actin cytoskeleton networks.  Biochem. Biophys. Res. Commun.  2017  488:614-620  PubMed ID: 28526408
14443  Yao J, Sugawara M, Obara H, Mizutani T, Takei M.  Distinct Motion of GFP-Tagged Histone Expressing Cells Under AC Electrokinetics in Electrode-Multilayered Microfluidic Device  IEEE Trans Biomed Circuits Syst  2017  11(6):1450-1458  PubMed ID: 28809711
9543  Mizutani T, Haga H, Kawabata K.  Data set for comparison of cellular dynamics between human AAVS1 locus-modified and wild-type cells.  Data Brief  2016  6:793-8  PubMed ID: 26937449
12924  Mizutani T, Furusawa K, Haga H, Kawabata K.  Heterogeneous filament network formation by myosin light chain isoforms effects on contractile energy output of single cardiomyocytes derived from human induced pluripotent stem cells  Regen Ther  2016  3:90-96  PubMed ID: 31245478
7253  Mizutani T, Li R, Haga H, Kawabata K.  Transgene integration into the human AAVS1 locus enhances myosin II-dependent contractile force by reducing expression of myosin binding subunit 85.  Biochem. Biophys. Res. Commun.  2015  465:270-4  PubMed ID: 26260320
14958  Ryosuke Yuchi, Jiafeng Yao, Takeomi Mizutani, Hiromichi Obara, Michiko Sugawara, Masahiro Takei  Analysis of dynamic cell movement under AC electric field in 3D multi-layered microchannel  2015 International Symposium on Micro-NanoMechatronics and Human Science (MHS)  2015  2015-11 
15954  Kiboku T, Katoh T, Nakamura A, Kitamura A, Kinjo M, Murakami Y, Takahashi M.  Nonmuscle myosin II folds into a 10S form via two portions of tail for dynamic subcellular localization  Genes Cells  2013  18(2):90-109  PubMed ID: 23237600
10360  Masaaki K Sato, Masayuki Takahashi, Michio Yazawa  Two Regions of the Tail Are Necessary for the Isoform-Specific Functions of Nonmuscle Myosin IIB  Mol Biol Cell  2007  18(3):1009-17  PubMed ID: 17202408
3378  Kimura, Makoto T, Mori, Takahiro, Conroy, Jeffrey, Nowak, Norma J, Satomi, Susumu, Tamai, Katsuyuki, Nagase, Hiroki  Two functional coding single nucleotide polymorphisms in STK15 (Aurora-A) coordinately increase esophageal cancer risk.  Cancer Res  2005  65:3548-54  PubMed ID: 15867347
2531  Masters JR, Thomson JA, Daly-Burns B, Reid YA, Dirks WG, Packer P, Toji LH, Ohno T, Tanabe H, Arlett CF, Kelland LR, Harrison M, Virmani A, Ward TH, Ayres KL, Debenham PG.  Short tandem repeat profiling provides an international reference standard for human cell lines.  Proc Natl Acad Sci U S A.  2001  98(14):8012-7  PubMed ID: 11416159
9009  H Imamura, S Takao, T Aikou  A Modified invasion-3-(4,5-dimethylthiazole-2-yl)-2,5-diphenyltetrazolium Bromide Assay for Quantitating Tumor Cell Invasion  Cancer Res  1994  54 (13), 3620-4  PubMed ID: 8012990
6243  Kodama S, Komatsu K, Okumura Y, Oshimura M.  Suppression of X-ray-induced chromosome aberrations in ataxia telangiectasia cells by introduction of a normal human chromosome 11.  Mutat Res  1992  293(1):31-7  PubMed ID: 1383808



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