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Cell No. : Cell Name
RCB0206 : MOLT-4  update : 2024/06/19
CommentT cell leukemia, the same patient as RCB1164 MOLT-3. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 85011413 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85011413 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (
Basic information Depositor ECACC
Originator Minowada, J.
Year of deposit 1987
Animal _human < Mammals
Genus Homo
Species sapiens
Gender Male
Age at sampling 19 years
Tissue blood
Disease name T cell leukemia
Classification cancer
History overseas, ECACC
Memo_1 Mycoplasma was eliminated with MC-210.
Lifespan infinite
Morphology lymphocyte-like
Cellosaurus(Expasy) CVCL_0013
deposit info
lot info
Medium Medium List
Culture type Suspension cells
Culture method 浮遊細胞の培養に関する一般的な注意(Japanese)
Culture medium RPMI1640 + 10% FBS
Antibiotics Free
Passage method dilution
Culture information Passage ratio 1 : 10 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Virus (HTLV-1) (-)
Isozyme LD, NP
Chromosome mode 83-182(50) : /94(6),95(14),96(10),97(2),98(7),99(2)/
STR(human) OK
deposit info
lot info
Reference information Reference 18
User's Publication 36

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5071  Drexler HG, Gaedicke G, Minowada J.  T-leukemia cell lines CCRF-CEM, HPB-ALL, JM and MOLT-4: changes in isoenzyme profiles during induction of differentiation.  Blut  1987  54(2):79-87  PubMed ID: 3493047   DOI: 10.1007/bf00321034
3544  Tighe L, Forster A, Clark DM, Boylston AW, Lavenir I, Rabbitts TH.  Unusual forms of T cell gamma mRNA in a human T cell leukemia cell line: implications for gamma gene expression.  Eur J Immunol  1987  17(12):1729-36  PubMed ID: 2961573   DOI: 10.1002/eji.1830171208
5042  Haruta Y, Seon BK.  Distinct human leukemia-associated cell surface glycoprotein GP160 defined by monoclonal antibody SN6.  Proc Natl Acad Sci U S A  1986  83(20):7898-902  PubMed ID: 3464004   DOI: 10.1073/pnas.83.20.7898
4748  Nakazawa S, Minowada J, Tsubota T, Sinks LF.  Profound radiosensitivity in "leukemic" T-cell lines and T-cell-type acute lymphoblastic leukemia demonstrated by sodium [51Cr]chromate labeling.  Cancer Res  1978  38(6):1661-6  PubMed ID: 306283  
5044  Han T, Dadey B, Minowada J.  Unique leukemic non-T/non-B lymphoid cell lines (REH and KM-3): absence of MLR-S and presence of suppressor cell activity for normal T-cell response.  J Clin Lab Immunol  1978  1(3):237-43  PubMed ID: 159361  
4747  Han T, Dadey B, Minowada J.  Stimulating capacity of fresh and cultured human leukaemic lymphoid and myeloid cells in 'one-way' mixed lymphocyte reaction.  Immunology  1977  33(4):543-51  PubMed ID: 144702  
164  Tsubota T, Minowada J, Nakazawa S, Sinks LF, Han T, Higby RJ, Pressman D.  Correlation of surface markers of cells of human lymphatic leukemias with disease type  J Natl Cancer Inst  1977  59(3):845-50  PubMed ID: 302342   DOI: 10.1093/jnci/59.3.845
4745  Pauly JL, Minowada J, Han T, Moore GE.  Disparity of mixed lymphocyte reactivity to cultured cells of human T and B lymphoid lines.  J Natl Cancer Inst  1975  54(3):557-62.  PubMed ID: 123598  
160  Hayata I, Oshimura M, Minowada J, Sandberg AA.  Chromosomal banding of cultured T and B lymphocytes  In Vitro  1975  11(6):361-8.  PubMed ID: 1081484   DOI: 10.1007/BF02616372
163  Kataoka K, Minowada J, Pressman D.  Electron microscope study on human lymphocyte-sheep erythrocyte rosettes  J Natl Cancer Inst  1975  55(6):1323-5  PubMed ID: 1239515   DOI: 10.1093/jnci/55.6.1323
4743  Itoh U, Minowada J, Moore GE, Pressman D.  Rosette-forming human lymphoid cell line (T-cell line molt). II. Ability for clonal growth.  J Natl Cancer Inst  1974  52(5):1403-7  PubMed ID: 4545482   DOI: 10.1093/jnci/52.5.1403
4744  Takada A, Takada Y, Minowada J.  Immunological functions of human T-lymphoid cell line (MOLT). I. Release of immunosuppressive factors from the mixture of MOLT-4 cells and sheep red blood cells.  J Exp Med  1974  140(2):538-48  PubMed ID: 4603015   DOI: 10.1084/jem.140.2.538
159  Huang CC, Hou Y, Woods LK, Moore GE, Minowada J.  Cytogenetic study of human lymphoid T-cell lines derived from lymphocytic leukemia  J Natl Cancer Inst  1974  53(3):655-60  PubMed ID: 4547172   DOI: 10.1093/jnci/53.3.655
4740  Sahai Srivastava BI, Minowada J.  Terminal deoxynucleotidyl transferase activity in a cell line (molt-4) derived from the peripheral blood of a patient with acute lymphoblastic leukemia.  Biochem Biophys Res Commun  1973  51(3):529-35  PubMed ID: 4512981   DOI: 10.1016/0006-291x(73)91346-6
4741  Gailani S, Minowada J, Silvernail P, Nussbaum A, Kaiser N, Rosen F, Shimaoka K.  Specific glucocorticoid binding in human hemopoietic cell lines and neoplastic tissue.  Cancer Res  1973  33(11):2653-7  PubMed ID: 4355977  
4742  Han T, Minowada J.  A unique 'leukaemic' T lymphoid cell line: absence of stimulating effect in mixed lymphocyte reaction. Lack of MLR-S in leukaemic T lymphoid cells.  Clin Exp Immunol  1973  15(4):535-41  PubMed ID: 4273215  
494  Minowada J, Onuma T, Moore, G E  Rosette-forming human lymphoid cell lines. I. Establishment and evidence for origin of thymus-derived lymphocytes.  J Natl Cancer Inst  1972  49:891-5  PubMed ID: 4567231  

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User's Publication
19998  Yoshida K, Fujita A, Narazaki H, Asano T, Itoh Y.  Drug resistance to nelarabine in leukemia cell lines might be caused by reduced expression of deoxycytidine kinase through epigenetic mechanisms  Cancer Chemother Pharmacol  2022  89(1):83-91  PubMed ID: 34825941   DOI: 10.1007/s00280-021-04373-4
12010  Teratake Y, Takashina T, Iijima K, Sakuma T, Yamamoto T, Ishizaka Y.  Development of a protein-based system for transient epigenetic repression of immune checkpoint molecule and enhancement of antitumour activity of natural killer cells.  Br. J. Cancer  2020    PubMed ID: 31959920   DOI: 10.1038/s41416-019-0708-y
13842  Hsieh HY, Jia W, Jin ZC, Kidoya H, Takakura N.  High expression of PSF1 promotes drug resistance and cell cycle transit in leukemia cells  Cancer Sci  2020  111(7):2400-2412  PubMed ID: 32391593   DOI: 10.1111/cas.14452
11964  Naito T, Ushirogawa H, Fukushima T, Tanaka Y, Saito M.  EOS, an Ikaros family zinc finger transcription factor, interacts with the HTLV-1 oncoprotein Tax and is downregulated in peripheral blood mononuclear cells of HTLV-1-infected individuals, irrespective of clinical statuses.  Virol. J.  2019    PubMed ID: 31856855   DOI: 10.1186/s12985-019-1270-1
11334  Komohara Y, Noyori O, Saito Y, Takeya H, Baghdadi M, Kitagawa F, Hama N, Ishikawa K, Okuno Y, Nosaka K, Seino KI, Matsuoka M, Suzu S.  Potential anti-lymphoma effect of M-CSFR inhibitor in adult T-cell leukemia/lymphoma.  J Clin Exp Hematop  2018    PubMed ID: 30541986   DOI: 10.3960/jslrt.18034
9736  Horlad H, Ohnishi K, Ma C, Fujiwara Y, Niino D, Ohshima K, Jinushi M, Matsuoka M, Takeya M, Komohara Y.  TIM-3 expression in lymphoma cells predicts chemoresistance in patients with adult T-cell leukemia/lymphoma.  Oncol Lett  2016  12:1519-1524  PubMed ID: 27446463   DOI: 10.3892/ol.2016.4774
12516  Ono M, Takimoto R, Osuga T, Okagawa Y, Hirakawa M, Yoshida M, Arihara Y, Uemura N, Hayasaka N, Miura S, Matsuno T, Tamura F, Sato Y, Sato T, Iyama S, Miyanishi K, Takada K, Kobune M, Kato J.  Targeting Notch-1 positive acute leukemia cells by novel fucose-bound liposomes carrying daunorubicin  Oncotarget  2016  7(25):38586-38597  PubMed ID: 27233074   DOI: 10.18632/oncotarget.9558
13994  Horlad H, Ma C, Yano H, Pan C, Ohnishi K, Fujiwara Y, Endo S, Kikukawa Y, Okuno Y, Matsuoka M, Takeya M, Komohara Y.  An IL-27/Stat3 axis induces expression of programmed cell death 1 ligands (PD-L1/2) on infiltrating macrophages in lymphoma  Cancer Sci  2016  107(11):1696-1704  PubMed ID: 27564404   DOI: 10.1111/cas.13065
9097  Masahiro Nagahama, Masahiro Shibutani, Soshi Seike, Mami Yonezaki, Teruhisa Takagishi, Masataka Oda, Keiko Kobayashi, Jun Sakurai  The p38 MAPK and JNK Pathways Protect Host Cells Against Clostridium Perfringens Beta-Toxin  Infect Immun  2013  81 (10), 3703-8  PubMed ID: 23876806   DOI: 10.1128/IAI.00579-13
8079  Gerelchuluun A, Hong Z, Sun L, Suzuki K, Terunuma T, Yasuoka K, Sakae T, Moritake T, Tsuboi K.  Induction of in situ DNA double-strand breaks and apoptosis by 200 MeV protons and 10 MV X-rays in human tumour cell lines.  Int. J. Radiat. Biol.  2011  87:57-70  PubMed ID: 20954835   DOI: 10.3109/09553002.2010.518201
12744  Okumura S, Saitoh H, Ishikawa T, Inouye K, Mizuki E.  Mode of action of parasporin-4, a cytocidal protein from Bacillus thuringiensis  Biochim Biophys Acta  2011  1808(6):1476-82  PubMed ID: 21073856   DOI: 10.1016/j.bbamem.2010.11.003
18753  Kimura S, Naganuma S, Susuki D, Hirono Y, Yamaguchi A, Fujieda S, Sano K, Itoh H.  Expression of microRNAs in squamous cell carcinoma of human head and neck and the esophagus: miR-205 and miR-21 are specific markers for HNSCC and ESCC  Oncol Rep  2010  23(6):1625-33  PubMed ID: 20428818   DOI: 10.3892/or_00000804
18826  Ohta T, Ikemoto Y, Saeki K, Koide T, Wakabayashi S.  Histidine-rich glycoprotein and concanavalin A synergistically stimulate the phosphatidylinositol 3-kinase-independent signaling pathway in leukocytes leading to increased cell adhesion and changes in cell morphology  Cell Immunol  2009  259(1):5-12  PubMed ID: 19535045   DOI: 10.1016/j.cellimm.2009.05.001
6132  Naito H, Kidoya H, Sato Y, Takakura N.  Induction and expression of anti-angiogenic vasohibins in the hematopoietic stem/progenitor cell population.  J Biochem  2009  145(5):653-9  PubMed ID: 19179360   DOI: 10.1093/jb/mvp021
6296  Shimadoi S, Takami A, Kondo Y, Okumura H, Nakao S.  Macrophage colony-stimulating factor enhances rituximab-dependent cellular cytotoxicity by monocytes.  Cancer Sci  2007  98(9):1368-72  PubMed ID: 17640305   DOI: 10.1111/j.1349-7006.2007.00544.x
9059  Y-C Jung, E Mizuki, T Akao, J-C Côté  Isolation and Characterization of a Novel Bacillus Thuringiensis Strain Expressing a Novel Crystal Protein With Cytocidal Activity Against Human Cancer Cells  J Appl Microbiol  2007  103 (1), 65-79  PubMed ID: 17584453   DOI: 10.1111/j.1365-2672.2006.03260.x
16902  Fujita K, Tsujimura A, Miyagawa Y, Kiuchi H, Matsuoka Y, Takao T, Takada S, Nonomura N, Okuyama A.  Isolation of germ cells from leukemia and lymphoma cells in a human in vitro model: potential clinical application for restoring human fertility after anticancer therapy  Cancer Res  2006  66(23):11166-71  PubMed ID: 17145860   DOI: 10.1158/0008-5472.CAN-06-2326
3413  Yamashita, Satoko, Katayama, Hideki, Saitoh, Hiroyuki, Akao, Tetsuyuki, Park, Yu Shin, Mizuki, Eiichi, Ohba, Michio, Ito, Akio  Typical Three-Domain Cry Proteins of Bacillus thuringiensis Strain A1462 Exhibit Cytocidal Activity on Limited Human Cancer Cells.  J Biochem  2005  138:663-72  PubMed ID: 16428294   DOI: 10.1093/jb/mvi177
2892  Katayama H, Yokota H, Akao T, Nakamura O, Ohba M, Mekada E, Mizuki E  Parasporin-1, a novel cytotoxic protein to human cells from non-insecticidal parasporal inclusions of Bacillus thuringiensis  J Biochem (Tokyo)  2005  137:17-25  PubMed ID: 15713879   DOI: 10.1093/jb/mvi003
3164  Takamatsu, Shinji, Inoue, Noboru, Katsumata, Toshiyuki, Nakamura, Katsumi, Fujibayashi, Yasuhisa, Takeuchi, Makoto  The relationship between the branch-forming glycosyltransferases and cell surface sugar chain structures.  Biochemistry  2005  44:6343-9  PubMed ID: 15835923   DOI: 10.1021/bi047606a
9048  Shiro Okumura, Hiroyuki Saitoh, Tomoyuki Ishikawa, Naoya Wasano, Satoko Yamashita, Ken-Ichi Kusumoto, Tetsuyuki Akao, Eiichi Mizuki, Michio Ohba, Kuniyo Inouye  Identification of a Novel Cytotoxic Protein, Cry45Aa, From Bacillus Thuringiensis A1470 and Its Selective Cytotoxic Activity Against Various Mammalian Cell Lines  J Agric Food Chem  2005  53 (16), 6313-8  PubMed ID: 16076112   DOI: 10.1021/jf0506129
4437  Hamasu T, Inanami O, Tsujitani M, Yokoyama K, Takahashi E, Kashiwakura I, Kuwabara M.  Post-irradiation hypoxic incubation of X-irradiated MOLT-4 cells reduces apoptotic cell death by changing the intracellular redox state and modulating SAPK/JNK pathways  Apoptosis  2005  10(3):557-67  PubMed ID: 15909118   DOI: 10.1007/s10495-005-1888-x
2823  Yamauchi, Ritsuko, Morita, Akimichi, Yasuda, Yoko, Grether-Beck, Susanne, Klotz, Lars-Oliver, Tsuji, Takuo, Krutmann, Jean  Different susceptibility of malignant versus nonmalignant human T cells toward ultraviolet A-1 radiation-induced apoptosis.  J Invest Dermatol  2004  122:477-83  PubMed ID: 15009733   DOI: 10.1046/j.0022-202X.2003.22106.x
2894  Ito, Akio, Sasaguri, Yasuyuki, Kitada, Sakae, Kusaka, Yoshitomo, Kuwano, Kyoko, Masutomi, Kenjiro, Mizuki, Eiichi, Akao, Tetsuyuki, Ohba, Michio  A Bacillus thuringiensis crystal protein with selective cytocidal action to human cells.  J Biol Chem  2004  279:21282-6  PubMed ID: 15026424   DOI: 10.1074/jbc.M401881200
2848  Feng, Xingmin, Chuhjo, Tatsuya, Sugimori, Chiharu, Kotani, Takeharu, Lu, Xuzhang, Takami, Akiyoshi, Takamatsu, Hiroyuki, Yamazaki, Hirohito, Nakao, Shinji  Diazepam-binding inhibitor-related protein 1: a candidate autoantigen in acquired aplastic anemia patients harboring a minor population of paroxysmal nocturnal hemoglobinuria-type cells.  Blood  2004  104:2425-31  PubMed ID: 15217832   DOI: 10.1182/blood-2004-05-1839
15089  Kuraya M, Matsushita M, Endo Y, Thiel S, Fujita T.  Expression of H-ficolin/Hakata antigen, mannose-binding lectin-associated serine protease (MASP)-1 and MASP-3 by human glioma cell line T98G  Int Immunol  2003  15(1):109-17  PubMed ID: 12502731   DOI: 10.1093/intimm/dxg008
2549  Nagai, Fusako, Hiyoshi, Yasuko, Sugimachi, Kumiko, Tamura, Hiro-Omi  Cytochrome P450 (CYP) expression in human myeloblastic and lymphoid cell lines.  Biol Pharm Bull  2002  25:383-5  PubMed ID: 11913539   DOI: 10.1248/bpb.25.383
6400  Hatano H, Kawashima H, Ogose A, Hotta T, Endo N.  A PCR-ELISA assay for the detection of disseminated osteosarcoma cells in a mouse metastatic model.  J Orthop Sci  2001  6(3):269-75  PubMed ID: 11484122   DOI: 10.1007/s007760100046
13875  Satoh JI, Kuroda Y.  Alpha-synuclein expression is up-regulated in NTera2 cells during neuronal differentiation but unaffected by exposure to cytokines and neurotrophic factors  Parkinsonism Relat Disord  2001  8(1):7-17  PubMed ID: 11472875   DOI: 10.1016/s1353-8020(00)00075-4
5968  Satoh J, Kuroda Y.  Beta-catenin expression in human neural cell lines following exposure to cytokines and growth factors.  Neuropathology  2000  20(2):113-23  PubMed ID: 10935448   DOI: 10.1046/j.1440-1789.2000.00293.x
1897  Lee DW, Akao T, Yamashita S, Katayama H, Maeda M, Saitoh H, Mizuki E, Ohba M.  Noninsecticidal parasporal proteins of a Bacillus thuringiensis serovar shandongiensis isolate exhibit a preferential cytotoxicity against human leukemic T cells  Biochem Biophys Res Commun  2000  272(1):218-23  PubMed ID: 10872830   DOI: 10.1006/bbrc.2000.2765
16572  Nomura Y, Inanami O, Takahashi K, Matsuda A, Kuwabara M.  2-Chloro-2'-deoxyadenosine induces apoptosis through the Fas/Fas ligand pathway in human leukemia cell line MOLT-4  Leukemia  2000  14(2):299-306  PubMed ID: 10673748   DOI: 10.1038/sj.leu.2401649
2440  Oshimi Y, Miyazaki S, Oda S.  ATP-induced Ca2+ response mediated by P2U and P2Y purinoceptors in human macrophages: signalling from dying cells to macrophages  Immunology  1999  98(2):220-7  PubMed ID: 10540220   DOI: 10.1046/j.1365-2567.1999.00858.x
2119  Yoshinobu Matsuo, Ayumi Okochi, Toshio Ariyasu, Emi Iimura and Tadao Ohno  Identification of Cell Lines with Variabel Numbers of Tandem Repeat(VNTR) Amplified by Polymerase Chain Reaction  Tiss Cult Res Commun  1996  15:211-219    DOI: 10.11418/jtca1981.15.4_21
1932  Tsurushima H, Ohno T, Tsuboi K, Yoshii Y, Meguro K, Nose T.  Low incidence of point mutation of N-ras oncogene in human gliomas  No Shinkei Geka  1995  23(7):581-6  PubMed ID: 7637839  
1500  Honma M, Kataoka E, Ohnishi K, Ohno T, Takeuchi M, Nomura N, Mizusawa H  A new DNA profiling system for cell line identification for use in cell banks in Japan.  In Vitro Cell Dev Biol  1992  28A:24-8  PubMed ID: 1730567   DOI: 10.1007/BF02631076

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