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Cell No. : Cell Name
RCB0205 : BU25 TK-  update : 2022/09/30
CommentThymidine kinase defective HeLa. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85022104 prior to order.
Comment from the depositor
Terms and conditions1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85022104 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor ECACC
Year of deposit 1987
Original cell HeLa
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Tissue cervix
Disease name cervical carcinoma
Classification cancer
History overseas
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_2320
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives MEM + 10% CS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Isozyme LD, NP
Chromosome mode 57-62(50) : 57(5),58(8),59(17),60(17),61(1),62(2)
STR(human) OK
Reference information Reference 4
User's Publication 1


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Reference
4656  Leung WC, Dubbs DR, Trkula D, Kit S.  Mitochondrial and herpesvirus-specific deoxypyrimidine kinases.  J Virol  1975  16(3):486-97  PubMed ID: 169387  
954  Kit S, Nakajima K, Dubbs, D R  Origin of thymidine kinase in adenovirus-infected human cell lines.  J Virol  1970  5:446-50  PubMed ID: 5452502  
953  Kit S, Dubbs, D R, Frearson, P M  HeLa cells resistant to bromodeoxyuridine and deficient in thymidine kinase activity.  Int J Cancer  1966  1:19-30  PubMed ID: 5902315   DOI: 10.1002/ijc.2910010105
150  Gey,G.O., Coffman,W.D. and Kubicek,M.T.  Tissue culture studies of the proliferative capacity of cervical carcinoma and normal epithelium.  Cancer Res  1952  12:264-265   

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User's Publication
6410  Danjoh I, Sone H, Noda N, Iimura E, Nagayoshi M, Saijo K, Hiroyama T, Nakamura Y.  Is parainfluenza virus a threatening virus for human cancer cell lines?  Hum Cell  2009  22(3):81-4  PubMed ID: 19624309   DOI: 10.1111/j.1749-0774.2009.00071.x



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