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Cell No. : Cell Name
RCB0205 : BU25 TK- update : 2023/05/25 |
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| Comment | Thymidine kinase defective HeLa. Since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECACC 85022104 prior to order. | |||
| Comment from the depositor | ||||
| Terms and conditions | 1) There is no restriction regarding use for basic researches. However, since “Terms and conditions” etc. for this cell line can be changed, the users of this cell line must refer to ECA85022104 prior to order. 2) In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC. | |||
| Remarks | ||||
| Order Form | Order Form(C-0005.pdf) MTA(C-0007.pdf) MTA(C-0007p.pdf) | |||
| Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp). | ||||
| Basic information | Depositor | ECACC | ||
| Year of deposit | 1987 | |||
| Original cell | HeLa | |||
| Animal | _human < Mammals | |||
| Genus | Homo | |||
| Species | sapiens | |||
| Gender | Female | |||
| Tissue | cervix | |||
| Disease name | cervical carcinoma | |||
| Classification | cancer | |||
| History | overseas | Lifespan | infinite | |
| Morphology | epithelial-like | |||
| Cellosaurus(Expasy) | CVCL_2320 | |||
| Medium | Medium List | Culture type | Adherent cells | |
| Culture medium | MEM + 10% CS | |||
| Antibiotics | Free | Passage method | 0.25% Trypsin | Culture information | Passage ratio | 1 : 8 split |
| SC frequency | Subculture : 2 times/week | |||
| Temperature | 37 ℃ | |||
| CO2 concentration | 5 % | |||
| Freeze medium | Medium + 10% DMSO | |||
| Freezing method | Slow freezing | |||
| Mycoplasma | (-) | |||
| Isozyme | LD, NP | |||
| Chromosome mode | 57-62(50) : 57(5),58(8),59(17),60(17),61(1),62(2) | |||
| STR(human) | OK | |||
| Reference information | Reference | 5 | ||
| User's Publication | 1 | |||
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