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細胞番号 : 細胞名
RCB0202 : A431  update : 2021/01/08
細胞特性(Comment:英)Human cell line derived from epidermoid carcinoma. Expressing EGF receptor.
細胞特性(日)ヒト上皮様細胞癌由来細胞株。EGF受容体を発現している。
細胞特性(寄託者記述:英)
細胞特性(寄託者記述:日)
使用条件(英)There is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
使用条件(日)基礎的研究につきましては、使用制限はありません。商業利用や特許取得を目的とする場合は、事前に必ず理研細胞バンクに連絡をすること。
備考(英)
備考(日)
提供申込書類(英) Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
提供申込書類(日) 依頼書C-0001.pdf   同意書(非営利学術目的)C-0003.pdf   同意書(営利目的)C-0003p.pdf  
提供同意書は、使用機関の種類や目的に応じて、非営利学術目的 (C-XXXX) と営利目的 (C-XXXXp) の2種類があります。該当する提供同意書をご使用ください(詳細)。特許等の取得及び商業利用等は事前に必ず(cellbank.brc@riken.jp)までご連絡ください。
提供手数料 手数料とお支払いについてはこちらをご覧ください。
細胞基本情報 寄託者 Abe, Shin-ichiro
寄託日 1987
動物種 _human < Mammals
属名 Homo
種名 sapiens
性別 Female
85 years
採取組織 epidermoid carcinoma
細胞分類 cancer
細胞寿命 infinite
細胞形態 epithelial-like
細胞培養・検査情報
寄託時情報
ロット情報
培地・試薬情報 培地・試薬一覧はこちらをご覧ください。
培養形態 Adherent cells
培地 DMEM (low glucose) + 10% FBS
抗生物質 Free
継代方法 0.25% trypsin
継代密度 1 : 8 split
継代・培地交換頻度 Subculture : once/week, Medium Renewal : 2 times/week
培養最適温度 37 ℃
二酸化炭素濃度 5 %
凍結培地 Medium + 10% DMSO
凍結方法 Slow freezing
マイコプラズマ (-)
個体識別検査 OK
ウィルス(HIV) (-)
アイソザイム検査 LD, NP, G6PD
染色体検査 72-82(50) : /74(5),75(7),76(8),77(10),78(7),79(3)/
画像情報
寄託時情報
ロット情報
文献情報 Reference(英) 1件
Reference(日) 1件
利用者成果(英) 32件
利用者成果(日) 0件

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Reference(英)
1026  Giard DJ, Aaronson SA, Todaro GJ, Arnstein P, Kersey JH, Dosik H, Parks WP.  In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.  J Natl Cancer Inst  1973  51(5):1417-23  PubMed ID: 4357758

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Reference(日)
1239  平井雅道,清水信義  バイオテクノロジ-素材としての培養細胞 増殖因子  蛋白質 核酸 酵素  1988  33:1232-1233 

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利用者成果(英)
20118  Abiko Y, Kurosawa K, Yamakawa H, Kumagai Y.  Activation of PTP1B/EGFR signaling and cytotoxicity during combined exposure to ambient electrophiles in A431 cells.  J Toxicol Sci  2021  46(4),177-185  PubMed ID: 33814511
13433  Setsuko Tsuboi, Takashi Jin   Shortwave-infrared (SWIR) fluorescence molecular imaging using indocyanine green–antibody conjugates for the optical diagnostics of cancerous tumours  RSC Adv  2020/1/1   
13442  Masahiro Nagahama, Keiko Kobayashi, Masaya Takehara  The Inhibitory Effect of Cholera Toxin B Subunit on Clostridium Perfringens Iota-Toxin-Induced Cytotoxicity  BPB Reports  2020/1/1   
10406  D. Merzhakupova, S. Pascarelli, P. Laurino  Epidermal Growth Factor single mutants highlighted by homologs cross-conservation approach differentially affect the EGF Receptor downstream pathway  bioRxiv  2020  2020-7-17 
13557  Luong NC, Abiko Y, Shibata T, Uchida K, Warabi E, Suzuki M, Noguchi T, Matsuzawa A, Kumagai Y.  Redox cycling of 9,10-phenanthrenequinone activates epidermal growth factor receptor signaling through S-oxidation of protein tyrosine phosphatase 1B  J Toxicol Sci  2020  45(6):349-36  PubMed ID: 32493877
13912  Yamamoto S, Nakanishi J.  Epidermal growth factor-gold nanoparticle conjugates-induced cellular responses: Effect of interfacial parameters between cell and nanoparticle  Anal Sci  2020  Dec 25  PubMed ID: 33390415
11376  Ren Q, Mohri K, Warashina S, Wada Y, Watanabe Y, Mukai H.  Improved Immuno-PET Imaging of HER2-Positive Tumors in Mice: Urokinase Injection-Triggered Clearance Enhancement of 64Cu-Trastuzumab.  Mol. Pharm.  2019    PubMed ID: 30633529
12371  Abiko Y, Nakai Y, Luong NC, Bianco CL, Fukuto JM, Kumagai Y.  Interaction of Quinone-Related Electron Acceptors with Hydropersulfide Na 2 S 2: Evidence for One-Electron Reduction Reaction  Chem Res Toxicol  2019  32(4):551-556  PubMed ID: 30719914
11076  Toyama K, Nomura W, Kobayakawa T, Tamamura H.  Delivery of a Proapoptotic Peptide to EGFR-Positive Cancer Cells by a Cyclic Peptide Mimicking the Dimerization Arm Structure of EGFR.  Bioconjug. Chem.  2018  29(6):2050-2057  PubMed ID: 29763296
4126  Osaki T, Nakanishi T, Aoki M, Omizu T, Nishiura D, Kitamura M.  Soluble Expression in Escherichia coli of a Single-Domain Antibody-Tumor Necrosis Factor α Fusion Protein Specific for Epidermal Growth Factor Receptor.  Monoclon Antib Immunodiagn Immunother  2018    PubMed ID: 29474158
4467  Toyama K, Kobayakawa T, Nomura W, Tamamura H.  Inhibition of EGFR Activation by Bivalent Ligands Based on a Cyclic Peptide Mimicking the Dimerization Arm Structure of EGFR.  Chem. Pharm. Bull.  2018  66(11):1083-1089  PubMed ID: 30381661
6021  Hasegawa G, Akatsuka K, Nakashima Y, Yokoe Y, Higo N, Shimonaka M.  Tamoxifen inhibits the proliferation of non‑melanoma skin cancer cells by increasing intracellular calcium concentration.  Int J Oncol  2018  53(5):2157-2166  PubMed ID: 30226592
10236  Sudo K, Niikura K, Iwaki K, Kohyama S, Fujiwara K, Doi N.  Human-derived fusogenic peptides for the intracellular delivery of proteins.  J Control Release  2017  255:1-11  PubMed ID: 28385674
14029  Shibuya Y, Haga N, Asano R, Nakazawa H, Hattori T, Takeda D, Sugiyama A, Kurotani R, Kumagai I, Umetsu M, Makabe K.  Generation of camelid VHH bispecific constructs via in-cell intein-mediated protein trans-splicing  Protein Eng Des Sel  2017  30(1):15-21  PubMed ID: 27881685
9117  Soshi Seike, Kazuaki Miyamoto, Keiko Kobayashi, Masaya Takehara, Masahiro Nagahama  Clostridium Perfringens Delta-Toxin Induces Rapid Cell Necrosis  PLoS One  2016  11 (1), e0147957  PubMed ID: 26807591
12681  Toyama K, Mizuguchi T, Nomura W, Tamamura H.  Functional evaluation of fluorescein-labeled derivatives of a peptide inhibitor of the EGF receptor dimerization  Bioorg Med Chem  2016  24(16):3406-12  PubMed ID: 27283787
12872  Niikura K, Horisawa K, Doi N.  Endosomal escape efficiency of fusogenic B18 and B55 peptides fused with anti-EGFR single chain Fv as estimated by nuclear translocation  J Biochem   2016  159(1):123-32.  PubMed ID: 26338729
12936  Hayashida Y, Ikeda Y, Sawada K, Kawai K, Kato T, Kakehi Y, Araki N.  Invention of a novel photodynamic therapy for tumors using a photosensitizing PI3K inhibitor  Int J Cancer  2016  139(3):700-11  PubMed ID: 26989815
12954  Hara-Chikuma M, Watanabe S, Satooka H.  Involvement of aquaporin-3 in epidermal growth factor receptor signaling via hydrogen peroxide transport in cancer cells  Biochem Biophys Res Commun  2016  471(4):603-9  PubMed ID: 26896765
14093  Batzaya Byambaa, Tomohiro Konno, Kazuhiko Ishihara  Photoinduced detachment of cells adhered on 2-methacryloyloxyethyl phosphorylcholine polymer with cell binding molecule through photocleavable linkage  Reactive and Functional Polymers  2016  104:30-37 
7928  Shoji Y, Miyamoto M, Ishikawa K, Yoshioka T, Mishra R, Ichinokawa K, Matsumura Y, Itoh T, Shinohara T, Hirano S, Kondo S.  The CD40-CD154 interaction would correlate with proliferation and immune escape in pancreatic ductal adenocarcinoma.  J Surg Oncol  2011  103:230-8  PubMed ID: 21337550
7956  Uchino T, Ikarashi Y, Nishimura T.  Effects of coating materials and size of titanium dioxide particles on their cytotoxicity and penetration into the cellular membrane.  J Toxicol Sci  2011  36:95-100  PubMed ID: 21297346
5355  Kishor Mazumder et al  Ursolic acid derivatives from Bangladeshi medicinal plant, Saurauja roxburghii: Isolation and cytotoxic activity against A431 and C6 glioma cell lines  Phytochemistry Letters  2011  4:287-291 
9083  Masahiro Nagahama, Mariko Umezaki, Masataka Oda, Keiko Kobayashi, Shigenobu Tone, Taiji Suda, Kazumi Ishidoh, Jun Sakurai  Clostridium Perfringens Iota-Toxin B Induces Rapid Cell Necrosis  Infect Immun  2011  79 (11), 4353-60  PubMed ID: 21911469
13818  Kikuji Yamashita, Shine-Od Dalkhsuren, Tatsuo Ishikawa, Kaori Sumida, Jun Ishibashi, Hiroyoshi Hosokawa, Akemichi Ueno, Fumio Nasu, Seiichiro Kitamura  Far Infrared Ray Radiation Inhibits the Proliferation of A549, HSC3 and Sa3 Cancer Cells through Enhancing the Expression of ATF3 Gene  Journal of Electromagnetic Analysis and Applications  2010  2:382-394 
8749  Matsumoto M, Yamaguchi Y, Seino Y, Hatakeyama A, Takei H, Niikura H, Ito K, Suzuki T, Sasano H, Yaegashi N, Hayashi S.  Estrogen signaling ability in human endometrial cancer through the cancer-stromal interaction.  Endocr. Relat. Cancer  2008  15:451-63  PubMed ID: 18508998
6452  Tsuji T, Kawada Y, Kai-Murozono M, Komatsu S, Han SA, Takeuchi K, Mizushima H, Miyazaki K, Irimura T.  Regulation of melanoma cell migration and invasion by laminin-5 and alpha3beta1 integrin (VLA-3).  Clin Exp Metastasis  2002  19(2):127-34  PubMed ID: 11964076
2371  Sato T, Iwai M, Sakai T, Sato H, Seiki M, Mori Y, Ito A.  Enhancement of membrane-type 1-matrix metalloproteinase (MT1-MMP) production and sequential activation of progelatinase A on human squamous carcinoma cells co-cultured with human dermal fibroblasts.  Br J Cancer  1999  80(8):1137-43  PubMed ID: 10376963
2196  Sato T, Nakajima H, Fujio K, Mori Y.  Enhancement of prostaglandin E2 production by epidermal growth factor requires the coordinate activation of cytosolic phospholipase A2 and cyclooxygenase 2 in human squamous carcinoma A431 cells.  Prostaglandins  1997  53(5):355-69  PubMed ID: 9247974
2069  Okada N, Fushimi M, Nagata Y, Fukunaga T, Tsutsumi Y, Nakagawa S, Mayumi T.  Evaluation of angiogenic inhibitors with an in vivo quantitative angiogenesis method using agarose microencapsulation and mouse hemoglobin enzyme-linked immunosorbent assay.  Jpn J Cancer Res  1996  87(9):952-7  PubMed ID: 8878458
2068  Okada N, Fushimi M, Nagata Y, Fukunaga T, Tsutsumi Y, Nakagawa S, Mayumi T.  A quantative in vivo method of analyzing human tumor-induced angiogenesis in mice using agarose microencapsulation and hemoglobin enzyme-linked immunosorbent assay.  Jpn J Cancer Res  1995  86(12):1182-8  PubMed ID: 8636008
1856  Shinohara Y, Yamamoto K, Kogure K, Ichihara J, Terada H.  Steady state transcript levels of the type II hexokinase and type 1 glucose transporter in human tumor cell lines.  Cancer Lett  1994  82(1):27-32  PubMed ID: 7518342

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