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Cell No. : Cell Name
RCB0202 : A431  update : 2022/11/14
CommentHuman cell line derived from epidermoid carcinoma. Expressing EGF receptor.
Comment from the depositor
Terms and conditionsThere is no restriction regarding use for basic researches. In relation to commercial use and use for patent filing, first of all please contact the RIKEN BRC.
Remarks
Order Form Order Form(C-0005.pdf)   MTA(C-0007.pdf)   MTA(C-0007p.pdf)  
Regarding MTA between user institutions and RIKEN BRC, there are two kinds of MTA, not-for-profit academic purpose (C-XXXX) and for-profit research purpose (C-XXXXp) , depending on the sort of user institutions and the purposes of use. Please use an appropriate MTA(to see). In relation to commercial use and use for patent filing, first of all Please contact RIKEN BRC (cellbank.brc@riken.jp).
Basic information Depositor Abe, Shin-ichiro
Year of deposit 1987
Animal human < Mammals
Genus Homo
Species sapiens
Gender Female
Age at sampling 85 years
Tissue epidermoid carcinoma
Classification cancer
Lifespan infinite
Morphology epithelial-like
Cellosaurus(Expasy) CVCL_0037
deposit info
lot info
Medium Medium List
Culture type Adherent cells
Medium and additives DMEM (low glucose) + 10% FBS
Antibiotics Free
Passage method 0.25% Trypsin
Culture information Passage ratio 1 : 8 split
SC frequency Subculture : once/week, Medium Renewal : 2 times/week
Temperature 37 ℃
CO2 concentration 5 %
Freeze medium Medium + 10% DMSO
Freezing method Slow freezing
Mycoplasma (-)
Virus (HIV) (-)
Isozyme LD, NP, G6PD
Chromosome mode 72-82(50) : /74(5),75(7),76(8),77(10),78(7),79(3)/
STR(human) OK
Images
deposit info
lot info
Reference information Reference 1
User's Publication 49


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Reference
1026  Giard DJ, Aaronson SA, Todaro GJ, Arnstein P, Kersey JH, Dosik H, Parks WP.  In vitro cultivation of human tumors: establishment of cell lines derived from a series of solid tumors.  J Natl Cancer Inst  1973  51(5):1417-23  PubMed ID: 4357758   DOI: 10.1093/jnci/51.5.1417

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User's Publication
16738  Soshi Seike, Masaya Takehara, Keiko Kobayashi, Masahiro Nagahama  Critical Residues of Clostridium perfringens Delta-Toxin for Oligomerization and Receptor Binding  BPB Reports  2022  5:66-69    DOI: 10.1248/bpbreports.5.3_66
20774  Kubota Y, Fujioka Y, Patil A, Takagi Y, Matsubara D, Iijima M, Momose I, Naka R, Nakai K, Noda NN, Takekawa M.  Qualitative differences in disease-associated MEK mutants reveal molecular signatures and aberrant signaling-crosstalk in cancer.  Nat Commun  2022  13(1):4063  PubMed ID: 35831322   DOI: 10.1038/s41467-022-31690-w
20881  Kiso-Farnè K, Tsuruyama T.  Epidermal growth factor receptor cascade prioritizes the maximization of signal transduction.  Sci Rep  2022  12(1):16950  PubMed ID: 36216834   DOI: 10.1038/s41598-022-20663-0
13912  Yamamoto S, Nakanishi J.  Epidermal growth factor-gold nanoparticle conjugates-induced cellular responses: Effect of interfacial parameters between cell and nanoparticle  Anal Sci  2021  37(5):741-745  PubMed ID: 33390415   DOI: 10.2116/analsci.20SCP16
20118  Abiko Y, Kurosawa K, Yamakawa H, Kumagai Y.  Activation of PTP1B/EGFR signaling and cytotoxicity during combined exposure to ambient electrophiles in A431 cells.  J Toxicol Sci  2021  46(4),177-185  PubMed ID: 33814511   DOI: 10.2131/jts.46.177
14839  Pascarelli S, Merzhakupova D, Uechi GI, Laurino P.  Binding of single-mutant epidermal growth factor (EGF) ligands alters the stability of the EGF receptor dimer and promotes growth signaling  J Biol Chem  2021  297(1):100872  PubMed ID: 34126069   DOI: 10.1016/j.jbc.2021.100872
20427  Tsumagari K, Chang CH, Ishihama Y.  A protocol for analyzing the protein terminome of human cancer cell line culture supernatants.  STAR Protoc  2021  2(3):100682  PubMed ID: 34377995   DOI: 10.1016/j.xpro.2021.100682
20442  Zhang A, Nakanishi J.  Improved anti-cancer effect of epidermal growth factor-gold nanoparticle conjugates by protein orientation through site-specific mutagenesis.  Sci Technol Adv Mater  2021  22(1):616-626  PubMed ID: 34512175   DOI: 10.1080/14686996.2021.1944783
10406  D. Merzhakupova, S. Pascarelli, P. Laurino  Epidermal Growth Factor single mutants highlighted by homologs cross-conservation approach differentially affect the EGF Receptor downstream pathway  bioRxiv  2020  2020-7-17    DOI: 10.1101/677393
13433  Setsuko Tsuboi, Takashi Jin   Shortwave-infrared (SWIR) fluorescence molecular imaging using indocyanine green–antibody conjugates for the optical diagnostics of cancerous tumours  RSC Adv  2020      DOI: 10.1039/D0RA04710D
13442  Masahiro Nagahama, Keiko Kobayashi, Masaya Takehara  The Inhibitory Effect of Cholera Toxin B Subunit on Clostridium Perfringens Iota-Toxin-Induced Cytotoxicity  BPB Reports  2020      DOI: 10.1248/bpbreports.3.4_146
13557  Luong NC, Abiko Y, Shibata T, Uchida K, Warabi E, Suzuki M, Noguchi T, Matsuzawa A, Kumagai Y.  Redox cycling of 9,10-phenanthrenequinone activates epidermal growth factor receptor signaling through S-oxidation of protein tyrosine phosphatase 1B  J Toxicol Sci  2020  45(6):349-36  PubMed ID: 32493877   DOI: 10.2131/jts.45.349
11730  Kobayashi Y, Iwakura Y, Sotoyama H, Kitayama E, Takei N, Someya T, Nawa H.  Clozapine-dependent inhibition of EGF/neuregulin receptor (ErbB) kinases.  Transl Psychiatry  2019    PubMed ID: 31371697   DOI: 10.1038/s41398-019-0519-1
9926  Setsuko Tsuboia and Takashi Jin  BRET based dual-colour (visible/near-infrared) molecular imaging using a quantum dot/EGFP–luciferase conjugate  RSC Adv  2019  9:34964-34971    DOI: 10.1039/C9RA07011G
12371  Abiko Y, Nakai Y, Luong NC, Bianco CL, Fukuto JM, Kumagai Y.  Interaction of Quinone-Related Electron Acceptors with Hydropersulfide Na 2 S 2: Evidence for One-Electron Reduction Reaction  Chem Res Toxicol  2019  32(4):551-556  PubMed ID: 30719914   DOI: 10.1021/acs.chemrestox.8b00158
11076  Toyama K, Nomura W, Kobayakawa T, Tamamura H.  Delivery of a Proapoptotic Peptide to EGFR-Positive Cancer Cells by a Cyclic Peptide Mimicking the Dimerization Arm Structure of EGFR.  Bioconjug. Chem.  2018  29(6):2050-2057  PubMed ID: 29763296   DOI: 10.1021/acs.bioconjchem.8b00250
4126  Osaki T, Nakanishi T, Aoki M, Omizu T, Nishiura D, Kitamura M.  Soluble Expression in Escherichia coli of a Single-Domain Antibody-Tumor Necrosis Factor α Fusion Protein Specific for Epidermal Growth Factor Receptor.  Monoclon Antib Immunodiagn Immunother  2018    PubMed ID: 29474158   DOI: 10.1089/mab.2017.0051
4467  Toyama K, Kobayakawa T, Nomura W, Tamamura H.  Inhibition of EGFR Activation by Bivalent Ligands Based on a Cyclic Peptide Mimicking the Dimerization Arm Structure of EGFR.  Chem. Pharm. Bull.  2018  66(11):1083-1089  PubMed ID: 30381661   DOI: 10.1248/cpb.c18-00539
6021  Hasegawa G, Akatsuka K, Nakashima Y, Yokoe Y, Higo N, Shimonaka M.  Tamoxifen inhibits the proliferation of non‑melanoma skin cancer cells by increasing intracellular calcium concentration.  Int J Oncol  2018  53(5):2157-2166  PubMed ID: 30226592   DOI: 10.3892/ijo.2018.4548
10236  Sudo K, Niikura K, Iwaki K, Kohyama S, Fujiwara K, Doi N.  Human-derived fusogenic peptides for the intracellular delivery of proteins.  J Control Release  2017  255:1-11  PubMed ID: 28385674   DOI: 10.1016/j.jconrel.2017.03.398
14029  Shibuya Y, Haga N, Asano R, Nakazawa H, Hattori T, Takeda D, Sugiyama A, Kurotani R, Kumagai I, Umetsu M, Makabe K.  Generation of camelid VHH bispecific constructs via in-cell intein-mediated protein trans-splicing  Protein Eng Des Sel  2017  30(1):15-21  PubMed ID: 27881685   DOI: 10.1093/protein/gzw057
9117  Soshi Seike, Kazuaki Miyamoto, Keiko Kobayashi, Masaya Takehara, Masahiro Nagahama  Clostridium Perfringens Delta-Toxin Induces Rapid Cell Necrosis  PLoS One  2016  11 (1), e0147957  PubMed ID: 26807591   DOI: 10.1371/journal.pone.0147957
12681  Toyama K, Mizuguchi T, Nomura W, Tamamura H.  Functional evaluation of fluorescein-labeled derivatives of a peptide inhibitor of the EGF receptor dimerization  Bioorg Med Chem  2016  24(16):3406-12  PubMed ID: 27283787   DOI: 10.1016/j.bmc.2016.05.026
12872  Niikura K, Horisawa K, Doi N.  Endosomal escape efficiency of fusogenic B18 and B55 peptides fused with anti-EGFR single chain Fv as estimated by nuclear translocation  J Biochem   2016  159(1):123-32.  PubMed ID: 26338729   DOI: 10.1093/jb/mvv083
12936  Hayashida Y, Ikeda Y, Sawada K, Kawai K, Kato T, Kakehi Y, Araki N.  Invention of a novel photodynamic therapy for tumors using a photosensitizing PI3K inhibitor  Int J Cancer  2016  139(3):700-11  PubMed ID: 26989815   DOI: 10.1002/ijc.30097
12954  Hara-Chikuma M, Watanabe S, Satooka H.  Involvement of aquaporin-3 in epidermal growth factor receptor signaling via hydrogen peroxide transport in cancer cells  Biochem Biophys Res Commun  2016  471(4):603-9  PubMed ID: 26896765   DOI: 10.1016/j.bbrc.2016.02.010
14093  Batzaya Byambaa, Tomohiro Konno, Kazuhiko Ishihara  Photoinduced detachment of cells adhered on 2-methacryloyloxyethyl phosphorylcholine polymer with cell binding molecule through photocleavable linkage  Reactive and Functional Polymers  2016  104:30-37    DOI: 10.1016/j.reactfunctpolym.2016.04.018
14188  Daisuke Takahashi, Takashi Nagao, Shota Sotokawa, Kazunobu Toshima  Target-selective photo-degradation of a sialyl Lewis a (sLea) conjugate and photo-cytotoxicity against sLea positive cancer cells using an anthraquinone-antibody hybrid  MedChemComm  2016  7:1224-1228    DOI: 10.1039/c6md00167j
14308  Osaki T, Wang CX, Tachibana T, Azuma M, Kitamura M, Nakanishi T.  Generation and Characterization of Rat Monoclonal Antibodies Against Epidermal Growth Factor Receptor  Monoclon Antib Immunodiagn Immunother  2015  34(6):418-22  PubMed ID: 26683181   DOI: 10.1089/mab.2015.0034
14980  Abiko Y, Luong NC, Kumagai Y.  A Biotin-PEAC5-maleimide labeling assay to detect electrophiles  J Toxicol Sci  2015  40(3):405-11  PubMed ID: 25972200   DOI: 10.2131/jts.40.405
15531  Osaki T, Fujisawa S, Kitaguchi M, Kitamura M, Nakanishi T.  Development of a bispecific antibody tetramerized through hetero-associating peptides  FEBS J  2015  282(22):4389-401  PubMed ID: 26337767   DOI: 10.1111/febs.13505
15865  Yutaka Hitomi, Toshiyuki Takeyasu, Masahito Kodera  Development of Green-Emitting Iron Complex-Based Fluorescent Probes for Intracellular Hydrogen Peroxide Imaging  Bulletin of the Chemical Society of Japan  2014  87:819-824    DOI: 10.1246/bcsj.20140055
7928  Shoji Y, Miyamoto M, Ishikawa K, Yoshioka T, Mishra R, Ichinokawa K, Matsumura Y, Itoh T, Shinohara T, Hirano S, Kondo S.  The CD40-CD154 interaction would correlate with proliferation and immune escape in pancreatic ductal adenocarcinoma.  J Surg Oncol  2011  103:230-8  PubMed ID: 21337550   DOI: 10.1002/jso.21812
7956  Uchino T, Ikarashi Y, Nishimura T.  Effects of coating materials and size of titanium dioxide particles on their cytotoxicity and penetration into the cellular membrane.  J Toxicol Sci  2011  36:95-100  PubMed ID: 21297346   DOI: 10.2131/jts.36.95
5355  Kishor Mazumder et al  Ursolic acid derivatives from Bangladeshi medicinal plant, Saurauja roxburghii: Isolation and cytotoxic activity against A431 and C6 glioma cell lines  Phytochemistry Letters  2011  4:287-291    DOI: 10.1016/j.phytol.2011.04.019
9083  Masahiro Nagahama, Mariko Umezaki, Masataka Oda, Keiko Kobayashi, Shigenobu Tone, Taiji Suda, Kazumi Ishidoh, Jun Sakurai  Clostridium Perfringens Iota-Toxin B Induces Rapid Cell Necrosis  Infect Immun  2011  79 (11), 4353-60  PubMed ID: 21911469   DOI: 10.1128/IAI.05677-11
13818  Kikuji Yamashita, Shine-Od Dalkhsuren, Tatsuo Ishikawa, Kaori Sumida, Jun Ishibashi, Hiroyoshi Hosokawa, Akemichi Ueno, Fumio Nasu, Seiichiro Kitamura  Far Infrared Ray Radiation Inhibits the Proliferation of A549, HSC3 and Sa3 Cancer Cells through Enhancing the Expression of ATF3 Gene  Journal of Electromagnetic Analysis and Applications  2010  2:382-394    DOI: 10.4236/jemaa.2010.26050
8749  Matsumoto M, Yamaguchi Y, Seino Y, Hatakeyama A, Takei H, Niikura H, Ito K, Suzuki T, Sasano H, Yaegashi N, Hayashi S.  Estrogen signaling ability in human endometrial cancer through the cancer-stromal interaction.  Endocr. Relat. Cancer  2008  15:451-63  PubMed ID: 18508998   DOI: 10.1677/ERC-07-0227
6452  Tsuji T, Kawada Y, Kai-Murozono M, Komatsu S, Han SA, Takeuchi K, Mizushima H, Miyazaki K, Irimura T.  Regulation of melanoma cell migration and invasion by laminin-5 and alpha3beta1 integrin (VLA-3).  Clin Exp Metastasis  2002  19(2):127-34  PubMed ID: 11964076   DOI: 10.1023/a:1014573204062
2371  Sato T, Iwai M, Sakai T, Sato H, Seiki M, Mori Y, Ito A.  Enhancement of membrane-type 1-matrix metalloproteinase (MT1-MMP) production and sequential activation of progelatinase A on human squamous carcinoma cells co-cultured with human dermal fibroblasts.  Br J Cancer  1999  80(8):1137-43  PubMed ID: 10376963   DOI: 10.1038/sj.bjc.6690477
17256  Takenouchi T, Kadosaka M, Shin SY, Munekata E.  Biological actions of the epidermal growth factors-like domain peptides of mouse schwannoma-derived growth factor and human amphiregulin  J Pept Res  1999  53(2):120-5  PubMed ID: 10195449   DOI: 10.1034/j.1399-3011.1999.00004.x
17247  Yasuyuki Suzuki, Yasuhiro Ozawa, Kazuo Murakami, Hitoshi Miyazaki  Inhibition of epidermal growth factor-induced stat1 activation by lysophosphatidic acid is mediated by pertussis toxin-sensitive g proteins and tyrosine phosphatase in human epidermoid carcinoma A431 cells  IUBMB Life  1998  44:979-987    DOI: 10.1080/15216549800202042
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2069  Okada N, Fushimi M, Nagata Y, Fukunaga T, Tsutsumi Y, Nakagawa S, Mayumi T.  Evaluation of angiogenic inhibitors with an in vivo quantitative angiogenesis method using agarose microencapsulation and mouse hemoglobin enzyme-linked immunosorbent assay.  Jpn J Cancer Res  1996  87(9):952-7  PubMed ID: 8878458   DOI: 10.1111/j.1349-7006.1996.tb02125.x
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1504  Hirano Y, Okuno M, Hayashi T, Goto K, Nakajima A.  Cell-attachment activities of surface immobilized oligopeptides RGD, RGDS, RGDV, RGDT, and YIGSR toward five cell lines  J Biomater Sci Polym Ed  1993  4(3):235-43  PubMed ID: 8476793   DOI: 10.1163/156856293x00546
15582  Yoshiaki Hirano, Motoya Okuno, Toshio Hayashi, Akio Nakajima  Liquid-Phase Synthesis and Cell Attachment Activity of Bioactive Pentapeptide YIGSR.  Polymer Journal  1992  24:465-472    DOI: 10.1295/polymj.24.465



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